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Psa biovar 3 is the most prevalent and virulent, causing frequent and severe outbreaks worldwide. While current treatments have low efficacy, bacteriophages emerge as possible environmentally safe alternative biocontrol agents. In this study, bacteriophage Brt_Psa3 was isolated from the soil of a kiwifruit orchard in Portugal. Morphologically, Brt_Psa3 forms clear plaques and has a Podoviral morphotype. The bacteriophage exhibited broad lytic activity against several plant-pathogenic\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    strains, including Psa isolates. The isolated bacteriophage has a latent period of 100\u00a0min, a burst size of 143 particles\/cell, and demonstrates stability at different temperatures and pH values found in kiwifruit orchards. In addition, Brt_Psa3 exhibited tolerance to UVA irradiation during 120\u00a0min of incubation. Brt_Psa3 belongs to the\n                    <jats:italic>Autographiviridae<\/jats:italic>\n                    family and\n                    <jats:italic>Ghunavirus<\/jats:italic>\n                    genus, based on full-genome nucleotide alignment and supported by phylogenetic analysis of structural proteins. The phage contains 51 open reading frames with no antibiotic resistance genes identified, within a genome of 40.509 base pairs. In vitro experiments with kiwifruit leaves demonstrated significant reduction of Psa levels (40%) on leaf surfaces, highlighting the bacteriophage\u2019s therapeutic potential in managing bacterial canker in kiwifruits.\n                  <\/jats:p>","DOI":"10.1007\/s00253-025-13613-z","type":"journal-article","created":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:43:23Z","timestamp":1760168603000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Lytic properties and genomic analysis of bacteriophage Brt_Psa3, targeting Pseudomonas syringae pv. actinidiae"],"prefix":"10.1007","volume":"109","author":[{"given":"Emil","family":"Gimranov","sequence":"first","affiliation":[]},{"given":"Hugo","family":"Oliveira","sequence":"additional","affiliation":[]},{"given":"Concei\u00e7\u00e3o","family":"Santos","sequence":"additional","affiliation":[]},{"given":"Lu\u00edsa","family":"Moura","sequence":"additional","affiliation":[]},{"given":"Joana","family":"Azeredo","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2025,10,11]]},"reference":[{"key":"13613_CR1","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1094\/PBIOMES-09-20-0070-R","volume":"5","author":"A Ares","year":"2021","unstructured":"Ares A, Pereira J, Garcia E, Costa J, Tiago I (2021) The leaf bacterial microbiota of female and male kiwifruit plants in distinct seasons: assessing the impact of Pseudomonas syringae pv. actinidiae. 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