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Phytohormones are major modulators of defensive responses in plants but the analysis of the hormonome associated with grapevine tolerance and susceptibility against this pathogen has not been elucidated. In this study, changes in hormonal profiling were compared between a tolerant (<jats:italic>Vitis rupestris<\/jats:italic>\u2009\u00d7\u2009<jats:italic>riparia<\/jats:italic> cv. 101-14 Millardet et de Grasset) and a susceptible (<jats:italic>Vitis vinifera<\/jats:italic> cv. Aragon\u00eaz) species upon <jats:italic>E. necator<\/jats:italic> infection. Control and PM-infected leaves were collected at 0, 6, 24, 96\u00a0h post-infection (hpi), and analysed through LC-MS\/MS. The results showed a distinct constitutive hormonome between tolerant and susceptible species. Constitutive high levels of salicylic acid (SA) and indole-3- acetic acid together with additional fast induction of SA within the first 6\u00a0hpi as well as constitutive low levels of jasmonates and abscisic acid may enable a faster and more efficient response towards the PM. The balance among the different phytohormones seems to be species-specific and fundamental in providing tolerance or susceptibility. These insights may be used to develop strategies for conventional breeding and\/or editing of genes involved in hormonal metabolism aiming at providing a durable resistance in grapevine against <jats:italic>E. necator<\/jats:italic>.\n<\/jats:p>","DOI":"10.1007\/s00344-022-10823-x","type":"journal-article","created":{"date-parts":[[2022,10,12]],"date-time":"2022-10-12T17:03:08Z","timestamp":1665594188000},"page":"3606-3614","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Hormone Changes in Tolerant and Susceptible Grapevine Leaves Under Powdery Mildew Infection"],"prefix":"10.1007","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5286-4148","authenticated-orcid":false,"given":"Rute","family":"Amaro","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0193-4385","authenticated-orcid":false,"given":"In\u00eas","family":"Diniz","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2450-1008","authenticated-orcid":false,"given":"Helena","family":"Santos","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8191-0916","authenticated-orcid":false,"given":"Diana","family":"Pimentel","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8952-537X","authenticated-orcid":false,"given":"Cec\u00edlia","family":"Rego","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5229-6913","authenticated-orcid":false,"given":"Axel","family":"Mith\u00f6fer","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7552-0164","authenticated-orcid":false,"given":"Ana Margarida","family":"Fortes","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2022,10,12]]},"reference":[{"issue":"6","key":"10823_CR1","doi-asserted-by":"publisher","first-page":"709","DOI":"10.1094\/MPMI-21-6-0709","volume":"21","author":"B Asselbergh","year":"2008","unstructured":"Asselbergh B, De Vleesschauwer D, H\u00f6fte M (2008) Global switches and fine-tuning-ABA modulates plant pathogen defense. 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