{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T21:40:56Z","timestamp":1767908456178,"version":"3.49.0"},"reference-count":62,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2023,1,7]],"date-time":"2023-01-07T00:00:00Z","timestamp":1673049600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2023,1,7]],"date-time":"2023-01-07T00:00:00Z","timestamp":1673049600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["SFRH\/BD\/147215\/2019"],"award-info":[{"award-number":["SFRH\/BD\/147215\/2019"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["UIDB\/04326\/2020"],"award-info":[{"award-number":["UIDB\/04326\/2020"]}]},{"DOI":"10.13039\/100014510","name":"European Maritime and Fisheries Fund","doi-asserted-by":"publisher","award":["AIM - MAR-02.01.01-FEAMP-0060"],"award-info":[{"award-number":["AIM - MAR-02.01.01-FEAMP-0060"]}],"id":[{"id":"10.13039\/100014510","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014510","name":"European Maritime and Fisheries Fund","doi-asserted-by":"publisher","award":["AIM - MAR-02.01.01-FEAMP-0060"],"award-info":[{"award-number":["AIM - MAR-02.01.01-FEAMP-0060"]}],"id":[{"id":"10.13039\/100014510","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014510","name":"European Maritime and Fisheries Fund","doi-asserted-by":"publisher","award":["AIM - MAR-02.01.01-FEAMP-0060"],"award-info":[{"award-number":["AIM - MAR-02.01.01-FEAMP-0060"]}],"id":[{"id":"10.13039\/100014510","id-type":"DOI","asserted-by":"publisher"}]},{"name":"IPMA - Instituto Portugu\u00eas do Mar e da Atmosfera"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Comp Physiol A"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The sensing of chemical cues is essential for several aspects of bivalve biology, such as the detection of food and pheromones. However, little is known about chemical communication systems in bivalves or the possible role of the osphradium as a chemosensory organ. To address this, we adapted an electrophysiological technique extensively used in vertebrates\u2014the electro-olfactogram\u2014to record from the osphradium in the Pacific oyster, <jats:italic>Magallana gigas<\/jats:italic>. This technique was validated using amino acids as stimulants. The osphradium proved to be sensitive to most proteinogenic <jats:sc>l<\/jats:sc>-amino acids tested, evoking tonic, negative, concentration-dependent \u2018electro-osphradiogram\u2019 (EOsG) voltage responses, with thresholds of detection in the range of 10<jats:sup>\u2212\u20096<\/jats:sup> to 10<jats:sup>\u2212\u20095<\/jats:sup>\u00a0M. Conversely, it was insensitive to <jats:sc>l<\/jats:sc>-arginine and <jats:sc>l<\/jats:sc>-glutamic acid. The current study supports the hypothesis that the osphradium is, indeed, a chemosensory organ. The \u2018electro-osphradiogram\u2019 may prove to be a powerful tool in the isolation and characterization of pheromones\u00a0and other important chemical cues in bivalve biology.<\/jats:p>","DOI":"10.1007\/s00359-022-01608-4","type":"journal-article","created":{"date-parts":[[2023,1,7]],"date-time":"2023-01-07T16:02:36Z","timestamp":1673107356000},"page":"391-401","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["What do oysters smell? Electrophysiological evidence that the bivalve osphradium is a chemosensory organ in the oyster, Magallana gigas"],"prefix":"10.1007","volume":"209","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1150-2757","authenticated-orcid":false,"given":"Ana","family":"Rato","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6324-5908","authenticated-orcid":false,"given":"Sandra","family":"Joaquim","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2191-7497","authenticated-orcid":false,"given":"Domit\u00edlia","family":"Matias","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3007-4647","authenticated-orcid":false,"given":"Peter C.","family":"Hubbard","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2023,1,7]]},"reference":[{"key":"1608_CR1","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1016\/j.neuron.2005.10.022","volume":"48","author":"BW Ache","year":"2005","unstructured":"Ache BW, Young JM (2005) Olfaction: diverse species, conserved principles. Neuron 48:417\u2013430. https:\/\/doi.org\/10.1016\/j.neuron.2005.10.022","journal-title":"Neuron"},{"key":"1608_CR2","doi-asserted-by":"publisher","first-page":"1692","DOI":"10.1126\/science.146.3652.1692","volume":"146","author":"E Aiello","year":"1964","unstructured":"Aiello E, Guideri G (1964) Nervous control of ciliary activity. Science 146:1692\u20131693. https:\/\/doi.org\/10.1126\/science.146.3652.1692","journal-title":"Science"},{"key":"1608_CR3","doi-asserted-by":"publisher","first-page":"350","DOI":"10.1080\/00288330.2014.909505","volume":"48","author":"TT Alipia","year":"2014","unstructured":"Alipia TT, Mae H, Dunphy BJ (2014) A non-invasive anaesthetic method for accessing the brood chamber of the chilean flat oyster (Ostrea chilensis). New Zeal J Mar Freshw Res 48:350\u2013355. https:\/\/doi.org\/10.1080\/00288330.2014.909505","journal-title":"New Zeal J Mar Freshw Res"},{"key":"1608_CR4","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1016\/j.aquaculture.2015.02.014","volume":"444","author":"B Arney","year":"2015","unstructured":"Arney B, Liu W, Forster IP et al (2015) Feasibility of dietary substitution of live microalgae with spray-dried Schizochytrium sp. or Spirulina in the hatchery culture of juveniles of the Pacific geoduck clam (Panopea generosa). Aquaculture 444:117\u2013133. https:\/\/doi.org\/10.1016\/j.aquaculture.2015.02.014","journal-title":"Aquaculture"},{"key":"1608_CR5","doi-asserted-by":"publisher","first-page":"479","DOI":"10.1007\/BF00603794","volume":"158","author":"AH Arzt","year":"1986","unstructured":"Arzt AH, Silver WL, Mason JR, Clark L (1986) Olfactory responses of aquatic and terrestrial tiger salamanders to airborne and waterborne stimuli. J Comp Physiol A 158:479\u2013487. https:\/\/doi.org\/10.1007\/BF00603794","journal-title":"J Comp Physiol A"},{"key":"1608_CR6","first-page":"219","volume-title":"Chemical ecology in aquatic systems","author":"J Atema","year":"2012","unstructured":"Atema J (2012) Aquatic odour dispersal fields: opportunities and limits of detection, communication, and navigation. In: Br\u00f6nmark C, Hansson L-A (eds) Chemical ecology in aquatic systems. Oxford University Press, Oxford, p 219"},{"key":"1608_CR7","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s11306-021-01820-4","volume":"17","author":"A Azizan","year":"2021","unstructured":"Azizan A, Alfaro AC, Young T, Venter L (2021) Beyond relaxed: magnesium chloride anaesthesia alters the circulatory metabolome of a marine mollusc (Perna canaliculus). Metabolomics 17:1\u201311. https:\/\/doi.org\/10.1007\/s11306-021-01820-4","journal-title":"Metabolomics"},{"key":"1608_CR8","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1242\/jeb.44.1.131","volume":"44","author":"DF Bailey","year":"1966","unstructured":"Bailey DF, Laverack MS (1966) Aspects of the neurophysiology of Buccinum undatum L. (Gastropoda). I. Central responses to stimulation of the osphradium. J Exp Biol 44:131\u2013148. https:\/\/doi.org\/10.1242\/jeb.44.1.131","journal-title":"J Exp Biol"},{"key":"1608_CR9","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1007\/BF00350330","volume":"123","author":"PG Beninger","year":"1995","unstructured":"Beninger PG, Donval A, Le Pennec M (1995) The osphradium in Placopecten magellanicus and Pecten maximus (Bivalvia, Pectinidae): histology, ultrastructure, and implications for spawning synchronisation. Mar Biol 123:121\u2013129. https:\/\/doi.org\/10.1007\/BF00350330","journal-title":"Mar Biol"},{"key":"1608_CR10","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1016\/S0022-0981(98)00187-7","volume":"235","author":"JG Boal","year":"1999","unstructured":"Boal JG, Golden DK, Mollusca, Cephalopoda (1999) Distance chemoreception in the common cuttlefish, Sepia officinalis (Mollusca, Cephalopoda). J Exp Mar Bio Ecol 235:307\u2013317. https:\/\/doi.org\/10.1016\/S0022-0981(98)00187-7","journal-title":"J Exp Mar Bio Ecol"},{"key":"1608_CR11","doi-asserted-by":"publisher","first-page":"342","DOI":"10.1016\/j.aquaculture.2007.12.004","volume":"275","author":"D Butt","year":"2008","unstructured":"Butt D, O\u2019Connor SJ, Kuchel R et al (2008) Effects of the muscle relaxant, magnesium chloride, on the Sydney rock oyster (Saccostrea glomerata). Aquaculture 275:342\u2013346. https:\/\/doi.org\/10.1016\/j.aquaculture.2007.12.004","journal-title":"Aquaculture"},{"key":"1608_CR12","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1007\/BF00656970","volume":"123","author":"J Caprio","year":"1978","unstructured":"Caprio J (1978) Olfaction and taste in the channel catfish: an electrophysiological study of the responses to amino acids and derivatives. J Comp Physiol\u00a0 A 123:357\u2013371. https:\/\/doi.org\/10.1007\/BF00656970","journal-title":"J Comp Physiol\u00a0 A"},{"key":"1608_CR13","doi-asserted-by":"publisher","first-page":"695","DOI":"10.1093\/chemse\/25.6.695","volume":"25","author":"M Chaput","year":"2000","unstructured":"Chaput M (2000) EOG responses in anaesthetized freely breathing rats. Chem Senses 25:695\u2013701. https:\/\/doi.org\/10.1093\/chemse\/25.6.695","journal-title":"Chem Senses"},{"key":"1608_CR14","doi-asserted-by":"publisher","first-page":"293","DOI":"10.1111\/j.1469-185x.1983.tb00391.x","volume":"58","author":"RP Croll","year":"1983","unstructured":"Croll RP (1983) Gastropod Chemoreception. Biol Rev 58:293\u2013319. https:\/\/doi.org\/10.1111\/j.1469-185x.1983.tb00391.x","journal-title":"Biol Rev"},{"key":"1608_CR15","doi-asserted-by":"publisher","first-page":"249","DOI":"10.1016\/0044-8486(92)90073-T","volume":"107","author":"SC Culloty","year":"1992","unstructured":"Culloty SC, Mulcahy MF (1992) An evaluation of anaesthetics for Ostrea edulis (L.). Aquaculture 107:249\u2013252. https:\/\/doi.org\/10.1016\/0044-8486(92)90073-T","journal-title":"Aquaculture"},{"key":"1608_CR16","doi-asserted-by":"publisher","first-page":"898","DOI":"10.1007\/s10886-008-9489-0","volume":"34","author":"CD Derby","year":"2008","unstructured":"Derby CD, Sorensen PW (2008) Neural processing, perception, and behavioral responses to natural chemical stimuli by fish and crustaceans. J Chem Ecol 34:898\u2013914. https:\/\/doi.org\/10.1007\/s10886-008-9489-0","journal-title":"J Chem Ecol"},{"key":"1608_CR17","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1002\/jemt.1070220402","volume":"22","author":"DG Emery","year":"1992","unstructured":"Emery DG (1992) Fine structure of olfactory epithelia of gastropod molluscs. Microsc Res Tech 22:307\u2013324. https:\/\/doi.org\/10.1002\/jemt.1070220402","journal-title":"Microsc Res Tech"},{"key":"1608_CR18","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1016\/j.cbpa.2009.05.006","volume":"154","author":"J Eom","year":"2009","unstructured":"Eom J, Jung YR, Park D (2009) F-series prostaglandin function as sex pheromones in the korean salamander, Hynobius leechii. Comp Biochem Physiol A Mol Integr Physiol 154:61\u201369. https:\/\/doi.org\/10.1016\/j.cbpa.2009.05.006","journal-title":"Comp Biochem Physiol A Mol Integr Physiol"},{"key":"1608_CR19","doi-asserted-by":"publisher","first-page":"1239","DOI":"10.1006\/jfbi.2002.2140","volume":"61","author":"P Frade","year":"2002","unstructured":"Frade P, Hubbard P, Barata E, Can\u00e1rio AV (2002) Olfactory sensitivity of the Mozambique tilapia to conspecific odours. J Fish Biol 61:1239\u20131254. https:\/\/doi.org\/10.1006\/jfbi.2002.2140","journal-title":"J Fish Biol"},{"key":"1608_CR20","doi-asserted-by":"publisher","first-page":"522","DOI":"10.2307\/1540384","volume":"149","author":"ZM Fuzessery","year":"1975","unstructured":"Fuzessery ZM, Childress JJ (1975) Comparative chemosensitivity to amino acids and their role in the feeding activity of Bathypelagic and Littoral Crustaceans. Biol Bull 149:522\u2013538. https:\/\/doi.org\/10.2307\/1540384","journal-title":"Biol Bull"},{"key":"1608_CR21","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1016\/j.jembe.2004.10.011","volume":"316","author":"M Garc\u00eda-Lavandeira","year":"2005","unstructured":"Garc\u00eda-Lavandeira M, Silva A, Abad M et al (2005) Effects of GABA and epinephrine on the settlement and metamorphosis of the larvae of four species of bivalve molluscs. J Exp Mar Bio Ecol 316:149\u2013156. https:\/\/doi.org\/10.1016\/j.jembe.2004.10.011","journal-title":"J Exp Mar Bio Ecol"},{"key":"1608_CR22","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1016\/0044-8486(84)90356-9","volume":"40","author":"MC Gibbons","year":"1984","unstructured":"Gibbons MC, Castagna M (1984) Serotonin as an inducer of spawning in six bivalve species. Aquaculture 40:189\u2013191. https:\/\/doi.org\/10.1016\/0044-8486(84)90356-9","journal-title":"Aquaculture"},{"key":"1608_CR23","volume-title":"Bivalve Molluscs: Biology, Ecology and Culture","author":"E Gosling","year":"2004","unstructured":"Gosling E (2004) Bivalve Molluscs: Biology, Ecology and Culture. Blackwell Publishing, Oxford, UK"},{"key":"1608_CR24","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/BF00043259","volume":"4","author":"TJ Hara","year":"1994","unstructured":"Hara TJ (1994) The diversity of chemical stimulation in fish olfaction and gustation. Rev Fish Biol Fish 4:1\u201335. https:\/\/doi.org\/10.1007\/BF00043259","journal-title":"Rev Fish Biol Fish"},{"key":"1608_CR25","doi-asserted-by":"publisher","first-page":"810","DOI":"10.1111\/j.0022-1112.2006.00967.x","volume":"68","author":"TJ Hara","year":"2006","unstructured":"Hara TJ (2006) Feeding behaviour in some teleosts is triggered by single amino acids primarily through olfaction. J Fish Biol 68:810\u2013825. https:\/\/doi.org\/10.1111\/j.0022-1112.2006.00967.x","journal-title":"J Fish Biol"},{"key":"1608_CR26","doi-asserted-by":"publisher","first-page":"457","DOI":"10.1098\/rstb.1985.0003","volume":"307","author":"G Haszprunar","year":"1985","unstructured":"Haszprunar G (1985a) The fine morphology of the osphradial sense organs of the Mollusca. 1. Gastropoda, Prosobranchia. Philos Trans R soc London B. Biol Sci 307:457\u2013496. https:\/\/doi.org\/10.1098\/rstb.1985.0003","journal-title":"Biol Sci"},{"key":"1608_CR27","doi-asserted-by":"publisher","first-page":"497","DOI":"10.1098\/rstb.1985.0004","volume":"307","author":"G Haszprunar","year":"1985","unstructured":"Haszprunar G (1985b) The fine morphology of the osphradial sense organs of the mollusca. II. Allogastropoda (Architectonicidae, Pyramidellidae). Philos Trans R Soc London B Biol Sci 307:497\u2013505. https:\/\/doi.org\/10.1098\/rstb.1985.0004","journal-title":"Philos Trans R Soc London B Biol Sci"},{"key":"1608_CR28","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1098\/rstb.1987.0002","volume":"315","author":"G Haszprunar","year":"1987","unstructured":"Haszprunar G (1987a) The fine morphology of the osphradial sense organs of the mollusca. III. Placophora and bivalvia. Philos Trans R Soc London B Biol Sci 315:37\u201361. https:\/\/doi.org\/10.1098\/rstb.1987.0002","journal-title":"Philos Trans R Soc London B Biol Sci"},{"key":"1608_CR29","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1098\/rstb.1987.0003","volume":"315","author":"G Haszprunar","year":"1987","unstructured":"Haszprunar G (1987b) The fine morphology of the osphradial sense organs of the mollusca. IV. Caudofoveata and Solenogastres. Philos Trans R Soc London B Biol Sci 315:63\u201373. https:\/\/doi.org\/10.1098\/rstb.1987.0003","journal-title":"Philos Trans R Soc London B Biol Sci"},{"key":"1608_CR30","doi-asserted-by":"publisher","first-page":"643","DOI":"10.1016\/j.ecoenv.2017.09.014","volume":"147","author":"JL Heerema","year":"2018","unstructured":"Heerema JL, Helbing CC, Pyle GG (2018) Use of electro-olfactography to measure olfactory acuity in the north american bullfrog (Lithobates (Rana) catesbeiana) tadpole. Ecotoxicol Environ Saf 147:643\u2013647. https:\/\/doi.org\/10.1016\/j.ecoenv.2017.09.014","journal-title":"Ecotoxicol Environ Saf"},{"key":"1608_CR31","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3791\/60962","volume":"2020","author":"P Hubbard","year":"2020","unstructured":"Hubbard P, Velez Z (2020) Extracellular multi-unit recording from the olfactory nerve of teleosts. J Vis Exp 2020:1\u201318. https:\/\/doi.org\/10.3791\/60962","journal-title":"J Vis Exp"},{"key":"1608_CR32","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1016\/S0166-445X(02)00002-4","volume":"60","author":"PC Hubbard","year":"2002","unstructured":"Hubbard PC, Barata EN, Canario AVM (2002) Possible disruption of pheromonal communication by humic acid in the goldfish, Carassius auratus. Aquat Toxicol 60:169\u2013183. https:\/\/doi.org\/10.1016\/S0166-445X(02)00002-4","journal-title":"Aquat Toxicol"},{"key":"1608_CR33","doi-asserted-by":"publisher","first-page":"2481","DOI":"10.1023\/a:1026357917887","volume":"29","author":"PC Hubbard","year":"2003","unstructured":"Hubbard PC, Barata EN, Can\u00e1rio AVM (2003a) Olfactory sensitivity of the gilthead seabream (Sparus auratus L.) to conspecfic body fluids. J ofChemical Ecol 29:2481\u20132498. https:\/\/doi.org\/10.1023\/a:1026357917887","journal-title":"J ofChemical Ecol"},{"key":"1608_CR34","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1093\/chemse\/28.3.207","volume":"28","author":"PC Hubbard","year":"2003","unstructured":"Hubbard PC, Barata EN, Can\u00e1rio AVM (2003b) Olfactory sensitivity to catecholamines and their metabolites in the goldfish. Chem Senses 28:207\u2013218. https:\/\/doi.org\/10.1093\/chemse\/28.3.207","journal-title":"Chem Senses"},{"key":"1608_CR35","doi-asserted-by":"publisher","first-page":"839","DOI":"10.1007\/s00359-011-0646-5","volume":"197","author":"PC Hubbard","year":"2011","unstructured":"Hubbard PC, Barata EN, Oz\u00f3rio ROA et al (2011) Olfactory sensitivity to amino acids in the blackspot sea bream (Pagellus bogaraveo): a comparison between olfactory receptor recording techniques in seawater. J Comp Physiol A Neuroethol Sensory Neural Behav Physiol 197:839\u2013849. https:\/\/doi.org\/10.1007\/s00359-011-0646-5","journal-title":"J Comp Physiol A Neuroethol Sensory Neural Behav Physiol"},{"key":"1608_CR36","doi-asserted-by":"publisher","first-page":"2148","DOI":"10.1111\/jfb.13297","volume":"90","author":"PC Hubbard","year":"2017","unstructured":"Hubbard PC, Baduy F, Saraiva JL et al (2017) High olfactory sensitivity to conspecific intestinal fluid in the chameleon cichlid Australoheros facetus: could faeces signal dominance? J Fish Biol 90:2148\u20132156. https:\/\/doi.org\/10.1111\/jfb.13297","journal-title":"J Fish Biol"},{"key":"1608_CR37","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1134\/S002209301406009X","volume":"50","author":"NN Kamardin","year":"2014","unstructured":"Kamardin NN (2014) Putative structural-functional evolution of osphradial sensory surface in aquatic prosobranchian molluscs. J Evol Biochem Physiol 50:539\u2013548. https:\/\/doi.org\/10.1134\/S002209301406009X","journal-title":"J Evol Biochem Physiol"},{"key":"1608_CR38","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1134\/S002209301501007X","volume":"51","author":"NN Kamardin","year":"2015","unstructured":"Kamardin NN, Lyubimtsev VA, Kornienko EL et al (2015) Osphradial chemosensory organ as a probable trigger of the cardiac system adaptive reaction to the effect of heavy metals in aquatic molluscs. J Evol Biochem Physiol 51:48\u201357. https:\/\/doi.org\/10.1134\/S002209301501007X","journal-title":"J Evol Biochem Physiol"},{"key":"1608_CR39","first-page":"180","volume":"44","author":"AO Kasumyan","year":"2004","unstructured":"Kasumyan AO (2004) The olfactory system in Fish: structure, function, and role in Behavior. J Ichthyol 44:180\u2013223","journal-title":"J Ichthyol"},{"key":"1608_CR40","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1016\/S0044-8486(96)01357-9","volume":"146","author":"J Knauer","year":"1996","unstructured":"Knauer J, Southgate PC (1996) Nutritional value of a spray-dried freshwater alga, Spongiococcum excentricum, for Pacific oyster (Crassostrea gigas) spat. Aquaculture 146:135\u2013146. https:\/\/doi.org\/10.1016\/S0044-8486(96)01357-9","journal-title":"Aquaculture"},{"key":"1608_CR41","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1093\/icb\/1.2.291","volume":"1","author":"AJ Kohn","year":"1961","unstructured":"Kohn AJ (1961) Chemoreception in gastropod molluscs. Integr Comp Biol 1:291\u2013308. https:\/\/doi.org\/10.1093\/icb\/1.2.291","journal-title":"Integr Comp Biol"},{"key":"1608_CR42","first-page":"205","volume":"20","author":"LR Kraemer","year":"1981","unstructured":"Kraemer LR (1981) The osphradial complex of two freshwater bivalves: histological evaluation and functional context. Malacologia 20:205\u2013216","journal-title":"Malacologia"},{"key":"1608_CR43","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1007\/978-3-319-22026-0_14","volume":"13","author":"O Kutsyna","year":"2016","unstructured":"Kutsyna O, Velez Z, Can\u00e1rio AVM et al (2016) Variation in urinary amino acids in the Mozambique Tilapia: a potential Signal of Dominance or Individuality? Chem Signals Vertebr 13:189\u2013203. https:\/\/doi.org\/10.1007\/978-3-319-22026-0_14","journal-title":"Chem Signals Vertebr"},{"key":"1608_CR44","doi-asserted-by":"publisher","first-page":"729","DOI":"10.1093\/chemse\/bjg055","volume":"28","author":"FL Lallou\u00e9","year":"2003","unstructured":"Lallou\u00e9 FL, Ayer-Le Li\u00e8vre CS, Sicard G (2003) Analysis of the functional maturation of olfactory neurons in Chicks before anda after Birth. Chem Senses 28:729\u2013737. https:\/\/doi.org\/10.1093\/chemse\/bjg055","journal-title":"Chem Senses"},{"key":"1608_CR45","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1093\/chemse\/bjs073","volume":"38","author":"H Lapid","year":"2013","unstructured":"Lapid H, Hummel T (2013) Recording odor-evoked response potentials at the human olfactory epithelium. Chem Senses 38:3\u201317. https:\/\/doi.org\/10.1093\/chemse\/bjs073","journal-title":"Chem Senses"},{"key":"1608_CR46","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1016\/j.aaf.2018.06.004","volume":"3","author":"J Li","year":"2018","unstructured":"Li J, Hubbard PC, Can\u00e1rio AVM (2018) Male zebrafish (Danio rerio)odorants attract females and induce spawning. Aquac Fish 3:139\u2013144. https:\/\/doi.org\/10.1016\/j.aaf.2018.06.004","journal-title":"Aquac Fish"},{"key":"1608_CR47","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1016\/j.jcz.2015.04.001","volume":"256","author":"DR Lindberg","year":"2015","unstructured":"Lindberg DR, Sigwart JD (2015) What is the molluscan osphradium? A reconsideration of homology. Zool Anz 256:14\u201321. https:\/\/doi.org\/10.1016\/j.jcz.2015.04.001","journal-title":"Zool Anz"},{"key":"1608_CR48","first-page":"485","volume":"105","author":"C Magel","year":"2007","unstructured":"Magel C, Wakefield K, Targett N, Brill R (2007) Activity in the pallial nerve of knobbed (Busycon carica) and channeled (Busycotypus canaliculatum) whelks recorded during exposure of the osphradium to odorant solutions. Fish Bull 105:485\u2013492","journal-title":"Fish Bull"},{"key":"1608_CR49","doi-asserted-by":"publisher","first-page":"323","DOI":"10.1016\/S0044-8486(99)00018-6","volume":"174","author":"MA McCausland","year":"1999","unstructured":"McCausland MA, Brown MR, Barrett SM et al (1999) Evaluation of live microalgae and microalgal pastes as supplementary food for juvenile pacific oysters (Crassostrea gigas). Aquaculture 174:323\u2013342. https:\/\/doi.org\/10.1016\/S0044-8486(99)00018-6","journal-title":"Aquaculture"},{"key":"1608_CR50","doi-asserted-by":"publisher","first-page":"261","DOI":"10.2983\/0730-8000(2008)27[261:SBOBSL]2.0.CO;2","volume":"27","author":"C Mes\u00edas-Gansbiller","year":"2008","unstructured":"Mes\u00edas-Gansbiller C, Bendimerad MEA, Rom\u00e1n G et al (2008) Settlement behavior of black scallop larvae (Chlamys varia, L.) in response to GABA, epinephrine and IBMX. J Shellfish Res 27:261\u2013264. https:\/\/doi.org\/10.2983\/0730-8000(2008)27[261:SBOBSL]2.0.CO;2","journal-title":"J Shellfish Res"},{"key":"1608_CR51","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1016\/j.aquaculture.2012.11.022","volume":"376\u2013379","author":"C Mes\u00edas-Gansbiller","year":"2013","unstructured":"Mes\u00edas-Gansbiller C, Silva A, Maneiro V et al (2013) Effects of chemical cues on larval settlement of the flat oyster (Ostrea edulis L.): a hatchery approach. Aquaculture 376\u2013379:85\u201389. https:\/\/doi.org\/10.1016\/j.aquaculture.2012.11.022","journal-title":"Aquaculture"},{"key":"1608_CR52","doi-asserted-by":"publisher","first-page":"496","DOI":"10.1039\/c7np00008a","volume":"34","author":"E Mollo","year":"2017","unstructured":"Mollo E, Garson MJ, Polese G et al (2017) Taste and smell in aquatic and terrestrial environments. Nat Prod Rep 34:496\u2013513. https:\/\/doi.org\/10.1039\/c7np00008a","journal-title":"Nat Prod Rep"},{"key":"1608_CR53","doi-asserted-by":"publisher","first-page":"241","DOI":"10.2331\/fishsci.61.241","volume":"61","author":"K Namba","year":"1995","unstructured":"Namba K, Kobayashi M, Aida S et al (1995) Persistent relaxation of the Adductor muscle of Oyster Crassostrea gigas Induced by Magnesium Ion. Fish Sci 61:241\u2013244. https:\/\/doi.org\/10.2331\/fishsci.61.241","journal-title":"Fish Sci"},{"key":"1608_CR54","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1016\/S0006-8993(97)00835-4","volume":"772","author":"L Nezlin","year":"1997","unstructured":"Nezlin L, Voronezhskaya E (1997) GABA-immunoreactive neurones and interactions of GABA with serotonin and FMRFamide in a peripheral sensory ganglion of the pond snail Lymnaea stagnalis. Brain Res 772:217\u2013225. https:\/\/doi.org\/10.1016\/S0006-8993(97)00835-4","journal-title":"Brain Res"},{"key":"1608_CR55","doi-asserted-by":"publisher","first-page":"bio035923","DOI":"10.1242\/bio.035923","volume":"7","author":"A Rato","year":"2018","unstructured":"Rato A, Joaquim S, Tavares TG et al (2018) Viability of dietary substitution of live microalgae with dry Ulva rigida in broodstock conditioning of the Pacific oyster (Crassostrea gigas). Biol Open 7:bio035923. https:\/\/doi.org\/10.1242\/bio.035923","journal-title":"Biol Open"},{"key":"1608_CR56","doi-asserted-by":"publisher","first-page":"1683","DOI":"10.1242\/jeb.00338","volume":"206","author":"SH Rolen","year":"2003","unstructured":"Rolen SH, Sorensen PW, Mattson D, Caprio J (2003) Polyamines as olfactory stimuli in the goldfish Carassius auratus. J Exp Biol 206:1683\u20131696. https:\/\/doi.org\/10.1242\/jeb.00338","journal-title":"J Exp Biol"},{"key":"1608_CR57","doi-asserted-by":"publisher","first-page":"152","DOI":"10.1002\/jemt.10133","volume":"58","author":"JW Scott","year":"2002","unstructured":"Scott JW, Scott-Johnson PE (2002) The electroolfactogram: a review of its history and uses. Microsc Res Tech 58:152\u2013160. https:\/\/doi.org\/10.1002\/jemt.10133","journal-title":"Microsc Res Tech"},{"key":"1608_CR58","doi-asserted-by":"publisher","first-page":"38","DOI":"10.13140\/RG.2.2.26793.11368.Keywords","volume":"4","author":"LRL Simone","year":"2021","unstructured":"Simone LRL (2021) The mollusk osphradium, structure and evolution. Maacopedia 4:38\u201349. https:\/\/doi.org\/10.13140\/RG.2.2.26793.11368.Keywords","journal-title":"Maacopedia"},{"key":"1608_CR59","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1051\/alr\/2009006","volume":"22","author":"M Suquet","year":"2009","unstructured":"Suquet M, Kermoysan G, De, Araya RG et al (2009) Anesthesia in Pacific oyster, Crassostrea gigas. Aquat Living Resour 22:29\u201334. https:\/\/doi.org\/10.1051\/alr\/2009006","journal-title":"Aquat Living Resour"},{"key":"1608_CR60","doi-asserted-by":"publisher","first-page":"756","DOI":"10.1086\/432145","volume":"78","author":"Z Velez","year":"2005","unstructured":"Velez Z, Hubbard PC, Barata EN, Can\u00e1rio AVM (2005) Evidence for functional asymmetry in the olfactory system of the senegalese sole (Solea senegalensis). Physiol Biochem Zool 78:756\u2013765. https:\/\/doi.org\/10.1086\/432145","journal-title":"Physiol Biochem Zool"},{"key":"1608_CR61","doi-asserted-by":"publisher","first-page":"336","DOI":"10.1016\/j.aquaculture.2007.02.029","volume":"265","author":"Z Velez","year":"2007","unstructured":"Velez Z, Hubbard PC, Hardege JD et al (2007) The contribution of amino acids to the odour of a prey species in the senegalese sole (Solea senegalensis). Aquaculture 265:336\u2013342. https:\/\/doi.org\/10.1016\/j.aquaculture.2007.02.029","journal-title":"Aquaculture"},{"key":"1608_CR62","doi-asserted-by":"publisher","first-page":"1743","DOI":"10.1242\/jeb.198.8.1743","volume":"198","author":"H Wedemeyer","year":"1995","unstructured":"Wedemeyer H, Schild D (1995) Chemosensitivity of the osphradium of the pond snail Lymnaea stagnalis. J Exp Biol 198:1743\u20131754. https:\/\/doi.org\/10.1242\/jeb.198.8.1743","journal-title":"J Exp Biol"}],"container-title":["Journal of Comparative Physiology A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00359-022-01608-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00359-022-01608-4\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00359-022-01608-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T04:03:23Z","timestamp":1681358603000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00359-022-01608-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,7]]},"references-count":62,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2023,5]]}},"alternative-id":["1608"],"URL":"https:\/\/doi.org\/10.1007\/s00359-022-01608-4","relation":{},"ISSN":["0340-7594","1432-1351"],"issn-type":[{"value":"0340-7594","type":"print"},{"value":"1432-1351","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1,7]]},"assertion":[{"value":"4 November 2022","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 December 2022","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 December 2022","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 January 2023","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing or financial interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}