{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:22:20Z","timestamp":1772119340548,"version":"3.50.1"},"reference-count":16,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T00:00:00Z","timestamp":1768953600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T00:00:00Z","timestamp":1768953600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["2022.04524.PTDC"],"award-info":[{"award-number":["2022.04524.PTDC"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006752","name":"Universidade do Porto","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100006752","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Histochem Cell Biol"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Peroxidasin (PXDN) is a multi-domain heme peroxidase that catalyzes sulfilimine cross-links in type IV collagen, a reaction essential for basement membrane stability. In addition to its structural role, PXDN has been implicated in oxidative stress regulation, fibrosis, and tumorigenesis. Antibody-based detection is critical for defining PXDN expression and localization, yet few commercial reagents have been rigorously validated, limiting reproducibility. Here, we assessed the specificity and performance of a commercially available anti-PXDN antibody (Abbexa, abx101906) in immunohistochemistry and immunocytochemistry applications. Validation was performed in formalin-fixed, paraffin-embedded human kidney tissue and in a primary kidney fibroblast line under\n                    <jats:italic>PXDN<\/jats:italic>\n                    silencing and overexpression conditions. The antibody yielded reproducible labeling patterns and demonstrated specificity across cellular contexts. These findings establish abx101906 as a reliable tool for immunofluorescence-based detection of PXDN. This validation provides a foundation for future studies of PXDN biology in kidney development, disease, and potential therapeutic targeting.\n                  <\/jats:p>","DOI":"10.1007\/s00418-025-02453-7","type":"journal-article","created":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T18:34:14Z","timestamp":1769020454000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Validation and performance assessment of a commercial anti-peroxidasin antibody"],"prefix":"10.1007","volume":"164","author":[{"given":"Isabel","family":"Brand\u00e3o","sequence":"first","affiliation":[]},{"given":"Roberto","family":"Silva","sequence":"additional","affiliation":[]},{"given":"B\u00e1rbara","family":"Gomes","sequence":"additional","affiliation":[]},{"given":"Jorge R.","family":"Almeida","sequence":"additional","affiliation":[]},{"given":"Jo\u00e3o Paulo","family":"Oliveira","sequence":"additional","affiliation":[]},{"given":"In\u00eas S.","family":"Alencastre","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2026,1,21]]},"reference":[{"issue":"9","key":"2453_CR1","doi-asserted-by":"publisher","first-page":"784","DOI":"10.1038\/nchembio.1038","volume":"8","author":"G Bhave","year":"2012","unstructured":"Bhave G, Cummings CF, Vanacore RM, Kumagai-Cresse C, Ero-Tolliver IA, Rafi M, Kang JS, Pedchenko V, Fessler LI, Fessler JH, Hudson BG (2012) Peroxidasin forms sulfilimine chemical bonds using hypohalous acids in tissue genesis. Nat Chem Biol 8(9):784\u2013790. https:\/\/doi.org\/10.1038\/nchembio.1038","journal-title":"Nat Chem Biol"},{"key":"2453_CR2","doi-asserted-by":"publisher","first-page":"100","DOI":"10.1016\/j.freeradbiomed.2022.02.026","volume":"182","author":"G Cheng","year":"2022","unstructured":"Cheng G, Shi R (2022) Mammalian peroxidasin (PXDN): from physiology to pathology. Free Radic Biol Med 182:100\u2013107. https:\/\/doi.org\/10.1016\/j.freeradbiomed.2022.02.026","journal-title":"Free Radic Biol Med"},{"issue":"12","key":"2453_CR3","doi-asserted-by":"publisher","first-page":"1682","DOI":"10.1016\/j.freeradbiomed.2008.09.009","volume":"45","author":"G Cheng","year":"2008","unstructured":"Cheng G, Salerno JC, Cao Z, Pagano PJ, Lambeth JD (2008) Identification and characterization of VPO1, a new animal heme-containing peroxidase. Free Radic Biol Med 45(12):1682\u20131694. https:\/\/doi.org\/10.1016\/j.freeradbiomed.2008.09.009","journal-title":"Free Radic Biol Med"},{"issue":"46","key":"2453_CR4","doi-asserted-by":"publisher","first-page":"24009","DOI":"10.1074\/jbc.M116.745935","volume":"291","author":"S Colon","year":"2016","unstructured":"Colon S, Bhave G (2016) Proprotein convertase processing enhances peroxidasin activity to reinforce collagen IV. J Biol Chem 291(46):24009\u201324016. https:\/\/doi.org\/10.1074\/jbc.M116.745935","journal-title":"J Biol Chem"},{"issue":"35","key":"2453_CR5","doi-asserted-by":"publisher","first-page":"21741","DOI":"10.1074\/jbc.M115.673996","volume":"290","author":"IA Ero-Tolliver","year":"2015","unstructured":"Ero-Tolliver IA, Hudson BG, Bhave G (2015) The ancient immunoglobulin domains of peroxidasin are required to form sulfilimine cross-links in collagen IV. J Biol Chem 290(35):21741\u201321748. https:\/\/doi.org\/10.1074\/jbc.M115.673996","journal-title":"J Biol Chem"},{"issue":"1","key":"2453_CR6","doi-asserted-by":"publisher","first-page":"331","DOI":"10.1073\/pnas.1318499111","volume":"111","author":"AL Fidler","year":"2014","unstructured":"Fidler AL, Vanacore RM, Chetyrkin SV, Pedchenko VK, Bhave G, Yin VP, Stothers CL, Rose KL, McDonald WH, Clark TA, Borza DB, Steele RE, Ivy MT, Aspirnauts, Hudson JK, Hudson BG (2014) A unique covalent bond in basement membrane is a primordial innovation for tissue evolution. Proc Natl Acad Sci U S A 111(1):331\u2013336. https:\/\/doi.org\/10.1073\/pnas.1318499111","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"10","key":"2453_CR7","doi-asserted-by":"publisher","first-page":"693","DOI":"10.1369\/0022155414545224","volume":"62","author":"SM Hewitt","year":"2014","unstructured":"Hewitt SM, Baskin DG, Frevert CW, Stahl WL, Rosa-Molinar E (2014) Controls for immunohistochemistry: the Histochemical Society\u2019s standards of practice for validation of immunohistochemical assays. J Histochem Cytochem 62(10):693\u2013697. https:\/\/doi.org\/10.1369\/0022155414545224","journal-title":"J Histochem Cytochem"},{"key":"2453_CR8","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10101565","author":"HA Kovacs","year":"2021","unstructured":"Kovacs HA, Lazar E, Varady G, Sirokmany G, Geiszt M (2021) Characterization of the proprotein convertase-mediated processing of peroxidasin and peroxidasin-like protein. Antioxidants (Basel). https:\/\/doi.org\/10.3390\/antiox10101565","journal-title":"Antioxidants (Basel)"},{"key":"2453_CR9","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1016\/j.freeradbiomed.2015.02.015","volume":"83","author":"E Lazar","year":"2015","unstructured":"Lazar E, Peterfi Z, Sirokmany G, Kovacs HA, Klement E, Medzihradszky KF, Geiszt M (2015) Structure-function analysis of peroxidasin provides insight into the mechanism of collagen IV crosslinking. Free Radic Biol Med 83:273\u2013282. https:\/\/doi.org\/10.1016\/j.freeradbiomed.2015.02.015","journal-title":"Free Radic Biol Med"},{"issue":"15","key":"2453_CR10","doi-asserted-by":"publisher","first-page":"3438","DOI":"10.1002\/j.1460-2075.1994.tb06649.x","volume":"13","author":"RE Nelson","year":"1994","unstructured":"Nelson RE, Fessler LI, Takagi Y, Blumberg B, Keene DR, Olson PF, Parker CG, Fessler JH (1994) Peroxidasin: a novel enzyme-matrix protein of Drosophila development. 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Am J Clin Pathol 121(2):190\u2013199. https:\/\/doi.org\/10.1309\/BRN7-CTX1-E84N-WWPL","journal-title":"Am J Clin Pathol"},{"issue":"17","key":"2453_CR15","doi-asserted-by":"publisher","first-page":"10876","DOI":"10.1074\/jbc.M114.632273","volume":"290","author":"M Soudi","year":"2015","unstructured":"Soudi M, Paumann-Page M, Delporte C, Pirker KF, Bellei M, Edenhofer E, Stadlmayr G, Battistuzzi G, Boudjeltia KZ, Furtmuller PG, Van Antwerpen P, Obinger C (2015) Multidomain human peroxidasin 1 is a highly glycosylated and stable homotrimeric high spin ferric peroxidase. 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Nat Methods 13(10):823\u2013827. https:\/\/doi.org\/10.1038\/nmeth.3995","journal-title":"Nat Methods"}],"container-title":["Histochemistry and Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00418-025-02453-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00418-025-02453-7","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00418-025-02453-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T18:34:15Z","timestamp":1769020455000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00418-025-02453-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,21]]},"references-count":16,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,12]]}},"alternative-id":["2453"],"URL":"https:\/\/doi.org\/10.1007\/s00418-025-02453-7","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-7575209\/v1","asserted-by":"object"}]},"ISSN":["1432-119X"],"issn-type":[{"value":"1432-119X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,21]]},"assertion":[{"value":"9 September 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 December 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 January 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"This study was conducted in accordance with the Declaration of Helsinki. The research protocol was approved by the institutional Health Ethics Commission (reference number CE-04-2023) and Data Protection Officer of S\u00e3o Jo\u00e3o Local Health Unit and University Hospital Centre (CHUSJ), Porto, Portugal.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}}],"article-number":"5"}}