{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T23:29:06Z","timestamp":1772753346375,"version":"3.50.1"},"reference-count":47,"publisher":"Springer Science and Business Media LLC","issue":"6","license":[{"start":{"date-parts":[[2005,3,3]],"date-time":"2005-03-03T00:00:00Z","timestamp":1109808000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Planta"],"published-print":{"date-parts":[[2005,8]]},"DOI":"10.1007\/s00425-005-1492-x","type":"journal-article","created":{"date-parts":[[2005,3,2]],"date-time":"2005-03-02T08:51:47Z","timestamp":1109753507000},"page":"815-822","source":"Crossref","is-referenced-by-count":71,"title":["Assessment of ploidy stability of the somatic embryogenesis process in Quercus suber L. using flow cytometry"],"prefix":"10.1007","volume":"221","author":[{"given":"J.","family":"Loureiro","sequence":"first","affiliation":[]},{"given":"G.","family":"Pinto","sequence":"additional","affiliation":[]},{"given":"T.","family":"Lopes","sequence":"additional","affiliation":[]},{"given":"J.","family":"Dole\u017eel","sequence":"additional","affiliation":[]},{"given":"C.","family":"Santos","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2005,3,3]]},"reference":[{"key":"1492_CR1","first-page":"326","volume-title":"A laboratory guide for cellular and molecular plant biology","author":"S Brown","year":"1991","unstructured":"Brown S, Bergounioux C, Tallet S, Marie D (1991) Flow cytometry of nuclei for ploidy and cell cycle analysis. In: Negrutiu I, Gharti-Chherti G (eds) A laboratory guide for cellular and molecular plant biology. Birkh\u00e4user, Basel, pp 326\u2013345"},{"key":"1492_CR2","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1007\/978-94-011-3983-0_4","volume-title":"Somatic cell genetics and molecular genetics of trees","author":"M Bueno","year":"1996","unstructured":"Bueno M, G\u00f3mez A, Vicente O, Manzanera J (1996) Stability in ploidy level during somatic embryogenesis in Quercus canariensis. In: Ajuha M, Bourjan W, Neale D (eds) Somatic cell genetics and molecular genetics of trees. Kluwer, Dordrecht, pp 23\u201328"},{"key":"1492_CR3","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1007\/978-94-017-3030-3_16","volume-title":"Somatic embryogenesis in woody plants","author":"M Bueno","year":"2000","unstructured":"Bueno M, G\u00f3mez A, Manzanera J (2000) Somatic and gametic embryogenesis in Quercus suber L. In: Jain S, Gupta P, Newton R (eds) Somatic embryogenesis in woody plants. Kluwer, Netherlands, pp 479\u2013508"},{"key":"1492_CR4","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1007\/s00299-003-0735-1","volume":"22","author":"Conde","year":"2004","unstructured":"Conde P, Loureiro J, Santos C (2004) Somatic embryogenesis and plant regeneration from leaves of Ulmus minor Mill. Plant Cell Rep 22:632\u2013639. DOI 10.1007\/s00299-003-0735-1","journal-title":"Plant Cell Rep"},{"key":"1492_CR5","unstructured":"Direc\u00e7\u00e3o Geral de Florestas (2001) Invent\u00e1rio Florestal Nacional, 3rd. Lisbon, pp 3\u2013162"},{"key":"1492_CR6","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1002\/pca.2800020402","volume":"2","author":"J Dole\u017eel","year":"1991","unstructured":"Dole\u017eel J (1991) Flow cytometric analysis of nuclear DNA content in higher plants. Phytochem Anal 2:143\u2013154","journal-title":"Phytochem Anal"},{"key":"1492_CR7","first-page":"285","volume":"38","author":"J Dole\u017eel","year":"1997","unstructured":"Dole\u017eel J (1997) Applications of flow cytometry for the study of plant genomes. J Appl Genet 38:285\u2013302","journal-title":"J Appl Genet"},{"key":"1492_CR8","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1002\/cyto.a.10013","volume":"51A","author":"Dole","year":"2003","unstructured":"Dole\u017eel J, Bartos J, Voglmayr H, Greilhuber J (2003) Nuclear DNA content and genome size of trout and human. Cytom Part A 51A:127\u2013128. DOI 10.1002\/cyto.a.10013","journal-title":"Cytom Part A"},{"key":"1492_CR9","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1002\/cyto.990190203","volume":"19","author":"J Dole\u017eel","year":"1995","unstructured":"Dole\u017eel J, G\u00f6hde W (1995) Sex Determination in Dioecious Plants Melandrium album and M. rubrum using high-resolution flow cytometry. Cytometry 19:103\u2013106","journal-title":"Cytometry"},{"key":"1492_CR10","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1007\/s004250050568","volume":"208","author":"El","year":"1999","unstructured":"El Maataoui M, Pichot C (1999) Nuclear and cell fusion cause polyploidy in the megagametophyte of common cypress, Cupressus sempervirens L. Planta 208:345\u2013351. DOI 10.1007\/s004250050568","journal-title":"Planta"},{"key":"1492_CR11","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1093\/aob\/mcf135","volume":"89","author":"E Emshwiller","year":"2002","unstructured":"Emshwiller E (2002) Ploidy levels among species in the \u2018Oxalis tuberosa Alliance\u2019 as inferred by flow cytometry. Ann Bot (Lond) 89:741\u2013753","journal-title":"Ann Bot (Lond)"},{"key":"1492_CR12","doi-asserted-by":"crossref","unstructured":"Endemann M, Hristoforoglu K, Stauber T, Wilhelm E (2001) Assessment of age-related polyploidy in Quercus robur L. somatic embryos and regenerated plants using DNA flow cytometry. Biol Plant (Prague) 44: 339\u2013345. DOI 10.1023\/A:1012426306493","DOI":"10.1023\/A:1012426306493"},{"key":"1492_CR13","unstructured":"FAO (1996) Food and agriculture organisation. Country report for Portugal. In: International conference and programme for plant genetic resources. FAO, p 15"},{"key":"1492_CR14","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1051\/forest:19960409","volume":"53","author":"J Favre","year":"1996","unstructured":"Favre J, Brown S (1996) A flow cytometric evaluation of the nuclear DNA content and GC percent in genomes of European oak species. Ann Sci For 53:915\u2013917","journal-title":"Ann Sci For"},{"key":"1492_CR15","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1007\/s001220050395","volume":"94","author":"Fourr","year":"1997","unstructured":"Fourr\u00e9 JL, Berger P, Niquet L, Andre P (1997) Somatic embryogenesis and somaclonal variation in Norway spruce: morphogenetic, cytogenetic and molecular approaches. Theor Appl Genet 94:159\u2013169. DOI 10.1007\/s001220050395","journal-title":"Theor Appl Genet"},{"key":"1492_CR16","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1126\/science.220.4601.1049","volume":"220","author":"DW Galbraith","year":"1983","unstructured":"Galbraith DW, Harkins KR, Maddox JM, Ayres NM, Sharma DP, Firoozabady E (1983) Rapid flow cytometric analysis of the cell-cycle in intact plant-tissues. Science 220:1049\u20131051","journal-title":"Science"},{"key":"1492_CR17","volume-title":"Current protocols in cytometry","author":"D Galbraith","year":"2002","unstructured":"Galbraith D, Lambert G, Macas J, Dole\u017eel J (2002) Analysis of nuclear DNA content and ploidy in higher plants. In: Robinson J, Darzynkiewicz Z, Dean P, Hibbs A, Orf\u00e3o A, Rabinovitch P, Wheeless L (eds) Current protocols in cytometry. Wiley, New York"},{"key":"1492_CR18","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1086\/297468","volume":"158","author":"FJ Gallego","year":"1997","unstructured":"Gallego FJ, Martinez I, Celestino C, Toribio M (1997) Testing somaclonal variation using RAPDs in Quercus suber L. somatic embryos. Int J Plant Sci 158:563\u2013567","journal-title":"Int J Plant Sci"},{"key":"1492_CR19","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1007\/978-94-011-3296-1_6","volume-title":"Genetics and breeding of ornamental species.","author":"T Geier","year":"1991","unstructured":"Geier T (1991) Chromosome variability in callus produced plants. In: Harding J, Singh F, Mol JNM (eds) Genetics and breeding of ornamental species. Kluwer, Dordrecht, pp 79\u2013106"},{"key":"1492_CR20","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1007\/BF00936335","volume":"158","author":"J Greilhuber","year":"1988","unstructured":"Greilhuber J (1988) Self-tanning\u2014a new and important source of stoichiometric error in cytophotometric determination of nuclear-DNA content in plants. Plant Syst Evol 158:87\u201396","journal-title":"Plant Syst Evol"},{"key":"1492_CR21","unstructured":"Hernandez I, Celestino C, Martinez J, Hornero J, Gallego J, Toribio M (1999) Induction of somatic embryogenesis in leaves from mature Quercus suber trees. Physiology and control of plant propagation in vitro. Report of activities, Eur Cost Action 822, Office for Official Publications of the European Communities, Krakow"},{"key":"1492_CR22","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1086\/320784","volume":"162","author":"J Hornero","year":"2001","unstructured":"Hornero J, Martinez I, Celestino C, Gallego FJ, Torres V, Toribio M (2001) Early checking of genetic stability of cork oak somatic embryos by AFLP analysis. Int J Plant Sci 162:827\u2013833","journal-title":"Int J Plant Sci"},{"key":"1492_CR23","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.1002\/j.1537-2197.1996.tb13892.x","volume":"83","author":"N Isabel","year":"1996","unstructured":"Isabel N, Boivin R, Levasseur C, Charest PM, Bousquet J, Tremblay FM (1996) Occurrence of somaclonal variation among somatic embryo-derived white spruces (Picea glauca, Pinaceae). Am J Bot 83:1121\u20131130","journal-title":"Am J Bot"},{"key":"1492_CR24","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1007\/978-94-017-3032-7_16","volume-title":"Somatic embryogenesis in woody plants","author":"K Ishii","year":"1999","unstructured":"Ishii K, Thakur R, Jain S (1999) Somatic embryogenesis and evaluation of variability in somatic seedlings of Quercus serrata by RAPD markers. In: Jain S, Gupta P, Newton R (eds) Somatic embryogenesis in woody plants. Kluwer, Dordrecht, pp 403\u2013414"},{"key":"1492_CR25","first-page":"671","volume-title":"Somatic embryogenesis in woody plants","author":"Y Kim","year":"2000","unstructured":"Kim Y (2000) Somatic embryogenesis in Quercus acutissima. In: Jain S, Gupta P, Newton R (eds) Somatic embryogenesis in woody plants. Kluwer, Dordrecht, pp 671\u2013686"},{"key":"1492_CR26","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1007\/BF02896685","volume":"38","author":"M Kubal\u00e1kov\u00e1","year":"1996","unstructured":"Kubal\u00e1kov\u00e1 M, Dole\u017eel J, Lebeda A (1996) Ploidy instability of embryogenic cucumber (Cucumis sativus L) callus culture. Biol Plant (Prague) 38:475\u2013480","journal-title":"Biol Plant (Prague)"},{"key":"1492_CR27","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1006\/anbo.2000.1337","volume":"87","author":"N Kudo","year":"2001","unstructured":"Kudo N, Kimura Y (2001) Patterns of endopolyploidy during seedling development in cabbage (Brassica oleracea L). Ann Bot (Lond) 87:275\u2013281","journal-title":"Ann Bot (Lond)"},{"key":"1492_CR28","unstructured":"Lopes T, Pinto G, Loureiro J, Costa A, Santos C (2003) Microsatellites as a tool for the determination of genetic stability in Quercus suber L. somatic embryos. Actas do X Congresso Nacional de Biotecnologia (BIOTEC\u20192003). Lisboa, Portugal, p 161"},{"key":"1492_CR29","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/0248-4900(93)90113-S","volume":"78","author":"D Marie","year":"1993","unstructured":"Marie D, Brown S (1993) A cytometric exercise in plant DNA histograms, with 2C values for 70 species. Biol Cell (Paris) 78:41\u201351","journal-title":"Biol Cell (Paris)"},{"key":"1492_CR30","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1093\/aob\/mcg105","volume":"92","author":"EA Moscone","year":"2003","unstructured":"Moscone EA, Baranyi M, Ebert I, Greilhuber J, Ehrendorfer F, Hunziker AT (2003) Analysis of nuclear DNA content in Capsicum (Solanaceae) by flow cytometry and Feulgen densitometry. Ann Bot (Lond) 92:21\u201329","journal-title":"Ann Bot (Lond)"},{"key":"1492_CR31","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1111\/j.1399-3054.1962.tb08052.x","volume":"15","author":"T Murashige","year":"1962","unstructured":"Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473\u2013497","journal-title":"Physiol Plant"},{"key":"1492_CR32","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1006\/anbo.2000.1187","volume":"86","author":"M Noirot","year":"2000","unstructured":"Noirot M, Barre P, Louarn J, Duperray C, Hamon S (2000) Nucleus-cytosol interactions\u2014a source of stoichiometric error in flow cytometric estimation of nuclear DNA content in plants. Ann Bot (Lond) 86:309\u2013316","journal-title":"Ann Bot (Lond)"},{"key":"1492_CR33","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1093\/aob\/mcf056","volume":"89","author":"M Noirot","year":"2002","unstructured":"Noirot M, Barre P, Louarn J, Duperray C, Hamon S (2002) Consequences of stoichiometric error on nuclear DNA content evaluation in Coffea liberica var. dewevrei using DAPI and propidium iodide. Ann Bot (Lond) 89:385\u2013389","journal-title":"Ann Bot (Lond)"},{"key":"1492_CR34","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1093\/aob\/mcg139","volume":"92","author":"M Noirot","year":"2003","unstructured":"Noirot M, Barre P, Duperray C, Louarn J, Hamon S (2003) Effects of caffeine and chlorogenic acid on propidium iodide accessibility to DNA: consequences on genome size evaluation in coffee tree. Ann Bot (Lond) 92:259\u2013264","journal-title":"Ann Bot (Lond)"},{"key":"1492_CR35","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1002\/cyto.990210412","volume":"21","author":"M Pfosser","year":"1995","unstructured":"Pfosser M, Amon A, Lelley T, Heberle-Bors E (1995) Evaluation of sensitivity of flow cytometry in detecting aneuploidy in wheat using disomic and ditelosomic wheat-rye addition lines. Cytometry 21:387\u2013393","journal-title":"Cytometry"},{"key":"1492_CR36","unstructured":"Pinto G, Amaral R, Santos C, Carnide O (2001) Somatic embryogenesis in calluses of leaves from 3\u00a0year old Quercus suber L. plants. Quality enhancement of plant production through tissue culture. In: Book of Abstracts. Second Meeting of the Cost 843 WG3. Office for Official Publications of the European Communities, Carcavelos"},{"key":"1492_CR37","doi-asserted-by":"crossref","unstructured":"Pinto G, Valentim H, Costa A, Castro S, Santos C (2002) Somatic embryogenesis in leaf callus from a mature Quercus suber L. tree. In Vitro Cell Dev Biol-Plant 38:569\u2013572. DOI 10.1079\/IVP2002352","DOI":"10.1079\/IVP2002352"},{"key":"1492_CR38","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1007\/s00122-004-1655-3","volume":"109","author":"Pinto","year":"2004","unstructured":"Pinto G, Loureiro J, Lopes T, Santos C (2004) Analysis of the genetic stability of Eucalyptus globulus Labill. somatic embryos by flow cytometry. Theor Appl Genet 109:580\u2013587. DOI 10.1007\/s00122-004-1655-3","journal-title":"Theor Appl Genet"},{"key":"1492_CR39","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1006\/anbo.2000.1255","volume":"86","author":"HJ Price","year":"2000","unstructured":"Price HJ, Hodnett G, Johnston JS (2000) Sunflower (Helianthus annuus) leaves contain compounds that reduce nuclear propidium iodide fluorescence. Ann Bot (Lond) 86:929\u2013934","journal-title":"Ann Bot (Lond)"},{"key":"1492_CR40","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1007\/s00299-002-0512-6","volume":"21","author":"Roux","year":"2003","unstructured":"Roux N, Toloza A, Radecki Z, Zapata-Arias F, Dole\u017eel J (2003) Rapid detection of aneuploidy in Musa using flow cytometry. Plant Cell Rep 21:483\u2013490. DOI 10.1007\/s00299-002-0512-6","journal-title":"Plant Cell Rep"},{"key":"1492_CR41","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1007\/BF02762241","volume":"4","author":"R Thakur","year":"1999","unstructured":"Thakur R, Gota S, Ishii K, Jain S (1999) Monitoring genetic stability in Quercus serrata Thunb. somatic embryogenesis using RAPD markers. J For Res 4:157\u2013160","journal-title":"J For Res"},{"key":"1492_CR42","unstructured":"Toribio M, Celestino C, Gallego J, Martinez J (1999) Induction of somatic embryogenesis in tissues from mature oak trees. Development of integrated systems for large scale propagation of elite plants using in vitro techniques. Report of activities, Eur Cost Action 822, Office for Official Publications of the European Communities, Luxemburg"},{"key":"1492_CR43","doi-asserted-by":"crossref","unstructured":"Trehin C, Planchais S, Glab N, Perennes C, Tregear J, Bergounioux C (1998) Cell cycle regulation by plant growth regulators: involvement of auxin and cytokinin in the re-entry of Petunia protoplasts into the cell cycle. Planta 206:215\u2013224. DOI: 10.1007\/s004250050393","DOI":"10.1007\/s004250050393"},{"key":"1492_CR44","doi-asserted-by":"crossref","first-page":"1373","DOI":"10.2307\/2656920","volume":"86","author":"L Tremblay","year":"1999","unstructured":"Tremblay L, Levasseur C, Tremblay FM (1999) Frequency of somaclonal variation in plants of black spruce (Picea mariana, Pinaceae) and white spruce (P. glauca, Pinaceae) derived from somatic embryogenesis and identification of some factors involved in genetic instability. Am J Bot 86:1373\u20131381","journal-title":"Am J Bot"},{"key":"1492_CR45","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1007\/s11627-000-0062-y","volume":"36","author":"E Wilhelm","year":"2000","unstructured":"Wilhelm E (2000) Somatic embryogenesis in oak (Quercus spp). In Vitro Cell Dev Biol-Plant 36:349\u2013357","journal-title":"In Vitro Cell Dev Biol-Plant"},{"key":"1492_CR46","unstructured":"Wilhelm E, Endemann M, Hristoforoglu K, Prewein C, Tutkova M (1999) Somatic embryogenesis in oak (Quercus robur L.) and production of artificial seeds. In: Proceedings of application of biotechnology to forest genetics. Biofor 99, Vitoria-Gasteiz"},{"key":"1492_CR47","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1139\/g98-006","volume":"41","author":"V Zoldos","year":"1998","unstructured":"Zoldos V, Pape\u0161 D, Brown S, Panaud O, Siljak-Yakovlev S (1998) Genome size and base composition of seven Quercus species: inter- and intra-population variation. Genome 41:162\u2013168","journal-title":"Genome"}],"container-title":["Planta"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00425-005-1492-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00425-005-1492-x\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00425-005-1492-x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,28]],"date-time":"2019-05-28T21:12:30Z","timestamp":1559077950000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00425-005-1492-x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,3,3]]},"references-count":47,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2005,8]]}},"alternative-id":["1492"],"URL":"https:\/\/doi.org\/10.1007\/s00425-005-1492-x","relation":{},"ISSN":["0032-0935","1432-2048"],"issn-type":[{"value":"0032-0935","type":"print"},{"value":"1432-2048","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,3,3]]}}}