{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T11:47:38Z","timestamp":1765885658183},"reference-count":48,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2009,6,2]],"date-time":"2009-06-02T00:00:00Z","timestamp":1243900800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Planta"],"published-print":{"date-parts":[[2009,7]]},"DOI":"10.1007\/s00425-009-0948-9","type":"journal-article","created":{"date-parts":[[2009,6,1]],"date-time":"2009-06-01T06:01:04Z","timestamp":1243836064000},"page":"429-439","source":"Crossref","is-referenced-by-count":55,"title":["Multiplicity of aspartic proteinases from Cynara cardunculus L."],"prefix":"10.1007","volume":"230","author":[{"given":"Ana Cristina","family":"Sarmento","sequence":"first","affiliation":[]},{"given":"Henrique","family":"Lopes","sequence":"additional","affiliation":[]},{"given":"Cl\u00e1udia S.","family":"Oliveira","sequence":"additional","affiliation":[]},{"given":"Rui","family":"Vitorino","sequence":"additional","affiliation":[]},{"given":"Bart","family":"Samyn","sequence":"additional","affiliation":[]},{"given":"Kjell","family":"Sergeant","sequence":"additional","affiliation":[]},{"given":"Griet","family":"Debyser","sequence":"additional","affiliation":[]},{"given":"Jozef","family":"Van Beeumen","sequence":"additional","affiliation":[]},{"given":"Pedro","family":"Domingues","sequence":"additional","affiliation":[]},{"given":"Francisco","family":"Amado","sequence":"additional","affiliation":[]},{"given":"Euclides","family":"Pires","sequence":"additional","affiliation":[]},{"given":"M. Ros\u00e1rio M.","family":"Domingues","sequence":"additional","affiliation":[]},{"given":"Marlene T.","family":"Barros","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2009,6,2]]},"reference":[{"key":"948_CR1","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1002\/pro.5560070109","volume":"7","author":"BM Beyer","year":"1998","unstructured":"Beyer BM, Dunn BM (1998) Prime region subsite specificity characterization of human cathepsin D: the dominant role of position 128. Protein Sci 7:88\u201395","journal-title":"Protein Sci"},{"key":"948_CR2","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1007\/978-1-4615-1871-6_29","volume":"362","author":"PE Brodelius","year":"1995","unstructured":"Brodelius PE, Cordeiro MC, Pais MS (1995) Aspartic proteinases (cyprosins) from Cynara cardunculus spp flavescens cv cardoon: purification, characterisation, and tissue-specific expression. Aspartic Proteinases 362:255\u2013266","journal-title":"Aspartic Proteinases"},{"key":"948_CR3","first-page":"405","volume":"125","author":"M Brodelius","year":"2005","unstructured":"Brodelius M, Hiraiwa M, Marttila S, Al Karadaghi S, Picaud S, Brodelius PE (2005) Immunolocalization of the saposin-like insert of plant aspartic proteinases exhibiting saposin C activity. Expression in young flower tissues and in barley seeds. Physiol Plant 125:405\u2013418","journal-title":"Expression in young flower tissues and in barley seeds. Physiol Plant"},{"key":"948_CR4","doi-asserted-by":"crossref","first-page":"13047","DOI":"10.1074\/jbc.M412076200","volume":"280","author":"P Castanheira","year":"2005","unstructured":"Castanheira P, Samyn B, Sergeant K, Clemente JC, Dunn BM, Pires E, Van Beeumen J, Faro C (2005) Activation, proteolytic processing, and peptide specificity of recombinant cardosin A. J Biol Chem 280:13047\u201313054","journal-title":"J Biol Chem"},{"key":"948_CR5","first-page":"1718","volume":"243","author":"RB Chow","year":"1968","unstructured":"Chow RB, Kassell B (1968) Bovine pepsinogen and pepsin I. Isolationm purification and some properties of pepsinogen. J Biol Chem 243:1718\u20131724","journal-title":"J Biol Chem"},{"key":"948_CR6","first-page":"683","volume":"47","author":"M Cordeiro","year":"1992","unstructured":"Cordeiro M, Jakob E, Puhan Z, Pais MS, Brodelius PE (1992) Milk clotting and proteolytic activities of purified cynarases from Cynara cardunculus\u2014a comparison to chymosin. Milchwiss Milk Sci Int 47:683\u2013687","journal-title":"Milchwiss Milk Sci Int"},{"key":"948_CR7","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1007\/BF00029855","volume":"24","author":"MC Cordeiro","year":"1994","unstructured":"Cordeiro MC, Xue ZT, Pietrzak M, Pais MS, Brodelius PE (1994) Isolation and characterisation of a cDNA from flowers of Cynara cardunculus encoding cyprosin (an aspartic proteinase) and its use to study the organ-specific expression of cyprosin. Plant Mol Biol 24:733\u2013741","journal-title":"Plant Mol Biol"},{"key":"948_CR8","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1111\/j.1432-1033.1997.t01-1-00695.x","volume":"243","author":"J Costa","year":"1997","unstructured":"Costa J, Ashford DA, Nimtz M, Bento I, Frazao C, Esteves CL, Faro CJ, Kervinen J, Pires E, Verissimo P, Wlodawer A, Carrondo MA (1997) The glycosylation of the aspartic proteinases from barley (Hordeum vulgare L) and cardoon (Cynara cardunculus L). Eur J Biochem 243:695\u2013700","journal-title":"Eur J Biochem"},{"key":"948_CR9","unstructured":"DeLano WL (2002) The PyMOL molecular graphics system. DeLano Scientific, San Carlos. Available at: http:\/\/www.pymol.org"},{"key":"948_CR10","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1098\/rstb.1975.0055","volume":"271","author":"JT Dingle","year":"1975","unstructured":"Dingle JT, Leaback DH (1975) Secretion of enzymes into pericellular environment. Philos Trans R Soc Lond B Biol Sci 271:315\u2013324","journal-title":"Philos Trans R Soc Lond B Biol Sci"},{"key":"948_CR11","doi-asserted-by":"crossref","first-page":"4431","DOI":"10.1021\/cr010167q","volume":"102","author":"BM Dunn","year":"2002","unstructured":"Dunn BM (2002) Structure and mechanism of the pepsin-like family of aspartic peptidases. Chem Rev 102:4431\u20134458","journal-title":"Chem Rev"},{"key":"948_CR12","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1021\/cr940283b","volume":"96","author":"RA Dwek","year":"1996","unstructured":"Dwek RA (1996) Glycobiology: toward understanding the function of sugars. Chem Rev 96:683\u2013720","journal-title":"Chem Rev"},{"key":"948_CR13","doi-asserted-by":"crossref","first-page":"38190","DOI":"10.1074\/jbc.M006093200","volume":"275","author":"C Egas","year":"2000","unstructured":"Egas C, Lavoura N, Resende R, Brito RMM, Pires E, de Lima MCP, Faro C (2000) The saposin-like domain of the plant aspartic proteinase precursor is a potent inducer of vesicle leakage. J Biol Chem 275:38190\u201338196","journal-title":"J Biol Chem"},{"key":"948_CR14","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1007\/978-1-4615-5373-1_58","volume":"436","author":"C Faro","year":"1998","unstructured":"Faro C, Ramalho-Santos M, Verissimo P, Pissarra J, Frazao C, Costa J, Lin XL, Tang J, Pires E (1998) Structural and functional aspects of cardosins. Aspartic Proteinases 436:423\u2013433","journal-title":"Aspartic Proteinases"},{"key":"948_CR15","doi-asserted-by":"crossref","first-page":"7426","DOI":"10.1021\/jf034573h","volume":"51","author":"J Fernandez-Salguero","year":"2003","unstructured":"Fernandez-Salguero J, Prados F, Calixto F, Vioque M, Sampaio P, Tejada L (2003) Use of recombinant cyprosin in the manufacture of ewe\u2019s milk cheese. J Agric Food Chem 51:7426\u20137430","journal-title":"J Agric Food Chem"},{"key":"948_CR16","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1080\/00365519209104656","volume":"52","author":"B Foltmann","year":"1992","unstructured":"Foltmann B (1992) Chymosin\u2014a short review on fetal and neonatal gastric proteases. Scand J Clin Lab Invest 52:65\u201379","journal-title":"Scand J Clin Lab Invest"},{"key":"948_CR17","doi-asserted-by":"crossref","first-page":"27694","DOI":"10.1074\/jbc.274.39.27694","volume":"274","author":"C Frazao","year":"1999","unstructured":"Frazao C, Bento I, Costa J, Soares CM, Verissimo P, Faro C, Pires E, Cooper J, Carrondo MA (1999) Crystal structure of cardosin A, a glycosylated and Arg-Gly-Asp-containing aspartic proteinase from the flowers of Cynara cardunculus L. J Biol Chem 274:27694\u201327701","journal-title":"J Biol Chem"},{"key":"948_CR18","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1086\/340729","volume":"77","author":"JS Fruton","year":"2002","unstructured":"Fruton JS (2002) A history of pepsin and related enzymes. Q Rev Biol 77:127\u2013147","journal-title":"Q Rev Biol"},{"key":"948_CR19","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/0014-5793(94)00935-X","volume":"352","author":"K Guruprasad","year":"1994","unstructured":"Guruprasad K, Tormakangas K, Kervinen J, Blundell TL (1994) Comparative modeling of barley-grain aspartic proteinase\u2014a structural rationale for observed hydrolytic specificity. FEBS Lett 352:131\u2013136","journal-title":"FEBS Lett"},{"key":"948_CR20","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.1016\/0031-9422(90)80090-4","volume":"29","author":"U Heimgartner","year":"1990","unstructured":"Heimgartner U, Pietrzak M, Geertsen R, Brodelius P, Figueiredo ACD, Pais MSS (1990) Purification and partial characterization of milk clotting proteases from flowers of Cynara cardunculus. Phytochemistry 29:1405\u20131410","journal-title":"Phytochemistry"},{"key":"948_CR21","doi-asserted-by":"crossref","first-page":"2305","DOI":"10.1021\/jm00112a001","volume":"34","author":"JR Huff","year":"1991","unstructured":"Huff JR (1991) Hiv protease\u2014a novel chemotherapeutic target for AIDS. J Med Chem 34:2305\u20132314","journal-title":"J Med Chem"},{"key":"948_CR22","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","volume":"227","author":"UK Laemmli","year":"1970","unstructured":"Laemmli UK (1970) Cleavage of structural proteins during assembly of head of bacteriophage-T4. Nature 227:680\u2013685","journal-title":"Nature"},{"key":"948_CR23","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1002\/1098-2787(2000)19:5<279::AID-MAS3>3.0.CO;2-G","volume":"19","author":"A Lapolla","year":"2000","unstructured":"Lapolla A, Fedele D, Traldi P (2000) The role of mass spectrometry in the study of non-enzymatic protein glycation in diabetes. Mass Spectrom Rev 19:279\u2013304","journal-title":"Mass Spectrom Rev"},{"key":"948_CR24","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1034\/j.1399-3054.1999.105324.x","volume":"105","author":"A Mutlu","year":"1999","unstructured":"Mutlu A, Gal S (1999) Plant aspartic proteinases: enzymes on the way to a function. Physiol Plant 105:569\u2013576","journal-title":"Physiol Plant"},{"key":"948_CR25","first-page":"349","volume-title":"Identification of mouse liver proteins on two-dimensional electrophoresis gels by matrix-assisted laser desorption ionization mass spectrometry of in situ enzymatic digests. 2nd Siena 2D electrophoresis\u2014from genome to proteome","author":"KL Oconnell","year":"1996","unstructured":"Oconnell KL, Stults JT (1996) Identification of mouse liver proteins on two-dimensional electrophoresis gels by matrix-assisted laser desorption ionization mass spectrometry of in situ enzymatic digests. 2nd Siena 2D electrophoresis\u2014from genome to proteome. Vch Publishers Inc, Siena, Italy, pp 349\u2013359"},{"key":"948_CR26","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1007\/s00709-008-0288-9","volume":"232","author":"CS Pereira","year":"2008","unstructured":"Pereira CS, da Costa DS, Pereira S, Nogueira FD, Albuquerque PM, Teixeira J, Faro C, Pissarra J (2008) Cardosins in postembryonic development of cardoon: towards an elucidation of the biological function of plant aspartic proteinases. Protoplasma 232:203\u2013213","journal-title":"Protoplasma"},{"key":"948_CR27","doi-asserted-by":"crossref","first-page":"2331","DOI":"10.3168\/jds.S0022-0302(99)75482-2","volume":"82","author":"A Picon","year":"1999","unstructured":"Picon A, Fernandez J, Gaya P, Medina M, Nunez M (1999) Short communication: stability of chymosin and cyprosins under milk-coagulation and cheese-ripening conditions. J Dairy Sci 82:2331\u20132333","journal-title":"J Dairy Sci"},{"key":"948_CR28","doi-asserted-by":"crossref","first-page":"2523","DOI":"10.1111\/j.1742-4658.2007.05787.x","volume":"274","author":"C Pimentel","year":"2007","unstructured":"Pimentel C, Van der Straeten D, Pires E, Faro C, Rodrigues-Pousada C (2007) Characterization and expression analysis of the aspartic protease gene family of Cynara cardunculus L. FEBS J 274:2523\u20132539","journal-title":"FEBS J"},{"key":"948_CR29","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/S0040-6031(02)00613-5","volume":"402","author":"DG Pina","year":"2003","unstructured":"Pina DG, Oliveira CS, Sarmento AC, Barros M, Pires E, Zhadan GG, Villar E, Gavilanes F, Shnyrov VL (2003) Thermostability of cardosin A from Cynara cardunculus L. Thermochimica Acta 402:123\u2013134","journal-title":"Thermochimica Acta"},{"key":"948_CR30","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1111\/j.1365-2621.2006.01271.x","volume":"42","author":"F Prados","year":"2007","unstructured":"Prados F, Pino A, Fernandez-Salguero J (2007) Effect of a powdered vegetable coagulant from cardoon Cynara cardunculus in the accelerated ripening of Manchego cheese. Int J Food Sci Technol 42:556\u2013561","journal-title":"Int J Food Sci Technol"},{"key":"948_CR31","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1007\/s004250050183","volume":"203","author":"M Ramalho-Santos","year":"1997","unstructured":"Ramalho-Santos M, Pissarra J, Verissimo P, Pereira S, Salema R, Pires E, Faro CJ (1997) Cardosin A, an abundant aspartic proteinase, accumulates in protein storage vacuoles in the stigmatic papillae of Cynara cardunculus L. Planta 203:204\u2013212","journal-title":"Planta"},{"key":"948_CR32","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1046\/j.1432-1327.1998.2550133.x","volume":"255","author":"M Ramalho-Santos","year":"1998","unstructured":"Ramalho-Santos M, Verissimo P, Cortes L, Samyn B, Van Beeumen J, Pires E, Faro C (1998) Identification and proteolytic processing of procardosin A. Eur J Biochem 255:133\u2013138","journal-title":"Eur J Biochem"},{"key":"948_CR33","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1038\/nmeth738","volume":"2","author":"B Samyn","year":"2005","unstructured":"Samyn B, Sergeant K, Castanheira P, Faro C, Van Beeumen J (2005) A new method for C-terminal sequence analysis in the proteomic era. Nat Methods 2:193\u2013200","journal-title":"Nat Methods"},{"key":"948_CR34","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/j.jasms.2003.11.003","volume":"15","author":"K Sandra","year":"2004","unstructured":"Sandra K, Devreese B, Van Beeumen J, Stals I, Claeyssens M (2004) The Q-trap mass spectrometer, a novel tool in the study of protein glycosylation. J Am Soc Mass Spectrom 15:413\u2013423","journal-title":"J Am Soc Mass Spectrom"},{"key":"948_CR35","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/S1381-1177(98)00066-6","volume":"5","author":"AC Sarmento","year":"1998","unstructured":"Sarmento AC, Silvestre L, Barros M, Pires E (1998) Cardosins A and B, two new enzymes available for peptide synthesis. J Mol Catal B Enzym 5:327\u2013330","journal-title":"J Mol Catal B Enzym"},{"key":"948_CR36","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.molcatb.2004.02.001","volume":"28","author":"AC Sarmento","year":"2004","unstructured":"Sarmento AC, Oliveira C, Pires E, Amado F, Barros M (2004a) Reverse hydrolysis by cardosin A: specificity considerations. J Mol Catal B Enzym 28:33\u201337","journal-title":"J Mol Catal B Enzym"},{"key":"948_CR37","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.molcatb.2004.09.001","volume":"31","author":"AC Sarmento","year":"2004","unstructured":"Sarmento AC, Oliveira CS, Pires EM, Halling PJ, Barros MT (2004b) Evaluation of cardosin A as a proteolytic probe in the presence of organic solvents. J Mol Catal B Enzym 31:137\u2013141","journal-title":"J Mol Catal B Enzym"},{"key":"948_CR38","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1016\/j.enzmictec.2005.06.010","volume":"38","author":"AC Sarmento","year":"2006","unstructured":"Sarmento AC, Oliveira CS, Duarte AS, Pires E, Barros MT (2006) Evaluation of cardosin A as a probe for limited proteolysis in non-aqueous environments\u2014complex substrates hydrolysis. Enzyme Microb Technol 38:415\u2013421","journal-title":"Enzyme Microb Technol"},{"key":"948_CR39","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.molcatb.2008.08.004","volume":"57","author":"AC Sarmento","year":"2009","unstructured":"Sarmento AC, Oliveira CS, Pereira A, Esteves VI, Moir AJG, Saraiva J, Pires E, Barros M (2009) Unfolding of cardosin A in organic solvents and detection of intermediaries. J Mol Catal B Enzym 57:115\u2013122","journal-title":"J Mol Catal B Enzym"},{"key":"948_CR40","doi-asserted-by":"crossref","first-page":"2369","DOI":"10.1002\/pmic.200501311","volume":"5","author":"K Sergeant","year":"2005","unstructured":"Sergeant K, Samyn B, Debyser G, Van Beeumen J (2005) De novo sequence analysis of N-terminal sulfonated peptides after in-gel guanidination. Proteomics 5:2369\u20132380","journal-title":"Proteomics"},{"key":"948_CR41","doi-asserted-by":"crossref","first-page":"2067","DOI":"10.1111\/j.1432-1033.2004.04136.x","volume":"271","author":"I Simoes","year":"2004","unstructured":"Simoes I, Faro C (2004) Structure and function of plant aspartic proteinases. Eur J Biochem 271:2067\u20132075","journal-title":"Eur J Biochem"},{"key":"948_CR42","doi-asserted-by":"crossref","first-page":"5786","DOI":"10.1111\/j.1742-4658.2005.04967.x","volume":"272","author":"I Simoes","year":"2005","unstructured":"Simoes I, Mueller EC, Otto A, Bur D, Cheung AY, Faro C, Pires E (2005) Molecular analysis of the interaction between cardosin A and phospholipase D alpha\u2014identification of RGD\/KGE sequences as binding motifs for C2 domains. FEBS J 272:5786\u20135798","journal-title":"FEBS J"},{"key":"948_CR43","doi-asserted-by":"crossref","first-page":"31358","DOI":"10.1074\/jbc.M702477200","volume":"282","author":"I Simoes","year":"2007","unstructured":"Simoes I, Faro R, Bur D, Faro C (2007) Characterization of recombinant CDR1, an Arabidopsis aspartic proteinase involved in disease resistance. J Biol Chem 282:31358\u201331365","journal-title":"J Biol Chem"},{"key":"948_CR44","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1016\/j.plaphy.2008.04.007","volume":"46","author":"K Takahashi","year":"2008","unstructured":"Takahashi K, Niwa H, Yokota N, Kubota K, Inoue H (2008) Widespread tissue expression of nepenthesin-like aspartic protease genes in Arabidopsis thaliana. Plant Physiol Biochem 46:724\u2013729","journal-title":"Plant Physiol Biochem"},{"key":"948_CR45","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.1002\/jsfa.3193","volume":"88","author":"L Tejada","year":"2008","unstructured":"Tejada L, Vioque M, Gomez R, Fernandez-Salguero J (2008) Effect of lyophilisation, refrigerated storage and frozen storage on the coagulant activity and microbiological quality of Cynara cardunculus L. extracts. J Sci Food Agric 88:1301\u20131306","journal-title":"J Sci Food Agric"},{"key":"948_CR46","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1385\/JMN:23:1-2:105","volume":"23","author":"R Vassar","year":"2004","unstructured":"Vassar R (2004) BACE1\u2014the beta-secretase enzyme in Alzheimer\u2019s disease. J Mol Neurosci 23:105\u2013113","journal-title":"J Mol Neurosci"},{"key":"948_CR47","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1111\/j.1432-1033.1996.00762.x","volume":"235","author":"P Verissimo","year":"1996","unstructured":"Verissimo P, Faro C, Moir AJG, Lin YZ, Tang J, Pires E (1996) Purification, characterization and partial amino acid sequencing of two new aspartic proteinases from fresh flowers of Cynara cardunculus L. Eur J Biochem 235:762\u2013768","journal-title":"Eur J Biochem"},{"key":"948_CR48","doi-asserted-by":"crossref","first-page":"16685","DOI":"10.1074\/jbc.274.24.16685","volume":"274","author":"PC White","year":"1999","unstructured":"White PC, Cordeiro MC, Arnold D, Brodelius PE, Kay J (1999) Processing, activity, and inhibition of recombinant cyprosin, an aspartic proteinase from cardoon (Cynara cardunculus). J Biol Chem 274:16685\u201316693","journal-title":"J Biol Chem"}],"container-title":["Planta"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00425-009-0948-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00425-009-0948-9\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00425-009-0948-9","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,28]],"date-time":"2019-05-28T21:12:12Z","timestamp":1559077932000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00425-009-0948-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,6,2]]},"references-count":48,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2009,7]]}},"alternative-id":["948"],"URL":"https:\/\/doi.org\/10.1007\/s00425-009-0948-9","relation":{},"ISSN":["0032-0935","1432-2048"],"issn-type":[{"value":"0032-0935","type":"print"},{"value":"1432-2048","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,6,2]]}}}