{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T15:37:24Z","timestamp":1770824244807,"version":"3.50.1"},"reference-count":68,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2019,3,15]],"date-time":"2019-03-15T00:00:00Z","timestamp":1552608000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Virchows Arch"],"published-print":{"date-parts":[[2019,7]]},"DOI":"10.1007\/s00428-019-02559-z","type":"journal-article","created":{"date-parts":[[2019,3,15]],"date-time":"2019-03-15T20:15:08Z","timestamp":1552680908000},"page":"3-11","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Invited review\u2014next-generation sequencing: a modern tool in cytopathology"],"prefix":"10.1007","volume":"475","author":[{"given":"Sinchita","family":"Roy-Chowdhuri","sequence":"first","affiliation":[]},{"given":"Pasquale","family":"Pisapia","sequence":"additional","affiliation":[]},{"given":"Manuel","family":"Salto-Tellez","sequence":"additional","affiliation":[]},{"given":"Spasenija","family":"Savic","sequence":"additional","affiliation":[]},{"given":"Mariantonia","family":"Nacchio","sequence":"additional","affiliation":[]},{"given":"Dario","family":"de Biase","sequence":"additional","affiliation":[]},{"given":"Giovanni","family":"Tallini","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1630-5805","authenticated-orcid":false,"given":"Giancarlo","family":"Troncone","sequence":"additional","affiliation":[]},{"given":"Fernando","family":"Schmitt","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2019,3,15]]},"reference":[{"key":"2559_CR1","doi-asserted-by":"publisher","first-page":"269","DOI":"10.1111\/cyt.12276","volume":"26","author":"M Salto-Tellez","year":"2015","unstructured":"Salto-Tellez M (2015) Diagnostic molecular cytopathology - a further decade of progress. Cytopathology 26:269\u2013270","journal-title":"Cytopathology"},{"key":"2559_CR2","doi-asserted-by":"publisher","first-page":"819","DOI":"10.1136\/jclinpath-2017-204561","volume":"70","author":"C Bellevicine","year":"2017","unstructured":"Bellevicine C, Malapelle U, Vigliar E, Pisapia P, Vita G, Troncone G (2017) How to prepare cytological samples for molecular testing. J Clin Pathol 70:819\u2013826","journal-title":"J Clin Pathol"},{"key":"2559_CR3","doi-asserted-by":"publisher","first-page":"1191","DOI":"10.5858\/arpa.2016-0117-RA","volume":"140","author":"S Roy-Chowdhuri","year":"2016","unstructured":"Roy-Chowdhuri S, Stewart J (2016) Preanalytic variables in cytology: lessons learned from next-generation sequencing-the MD Anderson experience. Arch Pathol Lab Med 140:1191\u20131199","journal-title":"Arch Pathol Lab Med"},{"key":"2559_CR4","doi-asserted-by":"publisher","first-page":"739","DOI":"10.2353\/jmoldx.2010.090238","volume":"12","author":"JK Killian","year":"2010","unstructured":"Killian JK, Walker RL, Suuriniemi M, Jones L, Scurci S, Singh P, Cornelison R, Harmon S, Boisvert N, Zhu J, Wang Y, Bilke S, Davis S, Giaccone G, Smith WI Jr, Meltzer PS (2010) Archival fine-needle aspiration cytopathology (FNAC) samples: untapped resource for clinical molecular profiling. J Mol Diagn 12:739\u2013745","journal-title":"J Mol Diagn"},{"key":"2559_CR5","doi-asserted-by":"publisher","first-page":"1189","DOI":"10.5858\/arpa.2016-0379-ED","volume":"140","author":"N Rekhtman","year":"2016","unstructured":"Rekhtman N, Roy-Chowdhuri S (2016) Cytology specimens: a goldmine for molecular testing. Arch Pathol Lab Med 140:1189\u20131190","journal-title":"Arch Pathol Lab Med"},{"key":"2559_CR6","doi-asserted-by":"publisher","first-page":"540","DOI":"10.1002\/cncy.21321","volume":"121","author":"MO Idowu","year":"2013","unstructured":"Idowu MO (2013) Epidermal growth factor receptor in lung cancer: the amazing interplay of molecular testing and cytopathology. Cancer Cytopathol 121:540\u2013543","journal-title":"Cancer Cytopathol"},{"key":"2559_CR7","doi-asserted-by":"publisher","first-page":"321","DOI":"10.5858\/arpa.2017-0388-CP","volume":"142","author":"NI Lindeman","year":"2018","unstructured":"Lindeman NI, Cagle PT, Aisner DL, Arcila ME, Beasley MB, Bernicker EH, Colasacco C, Dacic S, Hirsch FR, Kerr K, Kwiatkowski DJ, Ladanyi M, Nowak JA, Sholl L, Temple-Smolkin R, Solomon B, Souter LH, Thunnissen E, Tsao MS, Ventura CB, Wynes MW, Yatabe Y (2018) Updated molecular testing guideline for the selection of lung cancer patients for treatment with targeted tyrosine kinase inhibitors: guideline from the College of American Pathologists, the International Association for the Study of Lung Cancer, and the Association for Molecular Pathology. Arch Pathol Lab Med 142:321\u2013346","journal-title":"Arch Pathol Lab Med"},{"key":"2559_CR8","doi-asserted-by":"publisher","first-page":"284","DOI":"10.1053\/j.semdp.2013.11.008","volume":"30","author":"C Bellevicine","year":"2013","unstructured":"Bellevicine C, Vita GD, Malapelle U, Troncone G (2013) Applications and limitations of oncogene mutation testing in clinical cytopathology. Semin Diagn Pathol 30:284\u2013297","journal-title":"Semin Diagn Pathol"},{"key":"2559_CR9","doi-asserted-by":"publisher","first-page":"254","DOI":"10.1002\/cncy.21664","volume":"124","author":"S Roy-Chowdhuri","year":"2016","unstructured":"Roy-Chowdhuri S, Chow CW, Kane MK, Yao H, Wistuba II, Krishnamurthy S, Stewart J, Staerkel G (2016) Optimizing the DNA yield for molecular analysis from cytologic preparations. Cancer Cytopathol 124:254\u2013260","journal-title":"Cancer Cytopathol"},{"key":"2559_CR10","doi-asserted-by":"publisher","first-page":"659","DOI":"10.1002\/cncy.21597","volume":"123","author":"S Roy-Chowdhuri","year":"2015","unstructured":"Roy-Chowdhuri S, Goswami RS, Chen H, Patel KP, Routbort MJ, Singh RR, Broaddus RR, Barkoh BA, Manekia J, Yao H, Medeiros LJ, Staerkel G, Luthra R, Stewart J (2015) Factors affecting the success of next-generation sequencing in cytology specimens. Cancer Cytopathol 123:659\u2013668","journal-title":"Cancer Cytopathol"},{"key":"2559_CR11","doi-asserted-by":"publisher","first-page":"499","DOI":"10.1038\/modpathol.2016.228","volume":"30","author":"S Roy-Chowdhuri","year":"2017","unstructured":"Roy-Chowdhuri S, Chen H, Singh RR, Krishnamurthy S, Patel KP, Routbort MJ, Manekia J, Barkoh BA, Yao H, Sabir S, Broaddus RR, Medeiros LJ, Staerkel G, Stewart J, Luthra R (2017) Concurrent fine needle aspirations and core needle biopsies: a comparative study of substrates for next-generation sequencing in solid organ malignancies. Mod Pathol 30:499\u2013508","journal-title":"Mod Pathol"},{"key":"2559_CR12","doi-asserted-by":"publisher","first-page":"158","DOI":"10.1002\/cncy.21963","volume":"126","author":"NV Guseva","year":"2018","unstructured":"Guseva NV, Jaber O, Stence AA, Sompallae K, Bashir A, Sompallae R, Bossler AD, Jensen CS, Ma D (2018) Simultaneous detection of single-nucleotide variant, deletion\/insertion, and fusion in lung and thyroid carcinoma using cytology specimen and an RNA-based next-generation sequencing assay. Cancer Cytopathol 126:158\u2013169","journal-title":"Cancer Cytopathol"},{"key":"2559_CR13","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1002\/cncy.21771","volume":"125","author":"NP Velizheva","year":"2017","unstructured":"Velizheva NP, Rechsteiner MP, Wong CE, Zhong Q, R\u00f6ssle M, Bode B, Moch H, Soltermann A, Wild PJ, Tischler V (2017) Cytology smears as excellent starting material for next-generation sequencing-based molecular testing of patients with adenocarcinoma of the lung. Cancer Cytopathol 125:30\u201340","journal-title":"Cancer Cytopathol"},{"key":"2559_CR14","doi-asserted-by":"publisher","first-page":"402","DOI":"10.5858\/arpa.2016-0096-OA","volume":"141","author":"V Padmanabhan","year":"2017","unstructured":"Padmanabhan V, Steinmetz HB, Rizzo EJ, Erskine AJ, Fairbank TL, de Abreu FB, Tsongalis GJ, Tafe LJ (2017) Improving adequacy of small biopsy and fine-needle aspiration specimens for molecular testing by next-generation sequencing in patients with lung cancer: a quality improvement study at Dartmouth-Hitchcock Medical Center. Arch Pathol Lab Med 141:402\u2013409","journal-title":"Arch Pathol Lab Med"},{"key":"2559_CR15","doi-asserted-by":"publisher","first-page":"1200","DOI":"10.5858\/arpa.2016-0108-RA","volume":"140","author":"SK Tian","year":"2016","unstructured":"Tian SK, Killian JK, Rekhtman N, Benayed R, Middha S, Ladanyi M, Lin O, Arcila ME (2016) Optimizing workflows and processing of cytologic samples for comprehensive analysis by next-generation sequencing: memorial Sloan Kettering Cancer center experience. Arch Pathol Lab Med 140:1200\u20131205","journal-title":"Arch Pathol Lab Med"},{"key":"2559_CR16","doi-asserted-by":"publisher","first-page":"443","DOI":"10.5858\/apra.2017-0258-ED","volume":"142","author":"M Salto-Tellez","year":"2018","unstructured":"Salto-Tellez M (2018) More than a decade of molecular diagnostic cytopathology leading diagnostic and therapeutic decision-making. Arch Pathol Lab Med 142:443\u2013445","journal-title":"Arch Pathol Lab Med"},{"key":"2559_CR17","doi-asserted-by":"publisher","first-page":"271","DOI":"10.1111\/cyt.12265","volume":"26","author":"E Vigliar","year":"2015","unstructured":"Vigliar E, Malapelle U, de Luca C, Bellevicine C, Troncone G (2015) Challenges and opportunities of next-generation sequencing: a cytopathologist\u2019s perspective. Cytopathology 26:271\u2013283","journal-title":"Cytopathology"},{"key":"2559_CR18","doi-asserted-by":"publisher","first-page":"344","DOI":"10.1002\/cncy.21276","volume":"121","author":"A Dejmek","year":"2013","unstructured":"Dejmek A, Zendehrokh N, Tomaszewska M, Edsj\u00f6 A (2013) Preparation of DNA from cytological material: effects of fixation, staining, and mounting medium on DNA yield and quality. Cancer Cytopathol 121:344\u2013353","journal-title":"Cancer Cytopathol"},{"key":"2559_CR19","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1002\/cncy.21476","volume":"123","author":"MP Gailey","year":"2015","unstructured":"Gailey MP, Stence AA, Jensen CS, Ma D (2015) Multiplatform comparison of molecular oncology tests performed on cytology specimens and formalin-fixed, paraffin-embedded tissue. Cancer Cytopathol 123:30\u201339","journal-title":"Cancer Cytopathol"},{"key":"2559_CR20","doi-asserted-by":"publisher","first-page":"1036","DOI":"10.1038\/s41379-018-0006-x","volume":"31","author":"S Roy-Chowdhuri","year":"2018","unstructured":"Roy-Chowdhuri S, Mehrotra M, Bolivar AM, Kanagal-Shamanna R, Barkoh BA, Hannigan B, Zalles S, Ye W, Duose D, Broaddus R, Staerkel G, Wistuba I, Medeiros LJ, Luthra R (2018) Salvaging the supernatant: next generation cytopathology for solid tumor mutation profiling. Mod Pathol 31:1036\u20131045","journal-title":"Mod Pathol"},{"key":"2559_CR21","doi-asserted-by":"publisher","first-page":"314","DOI":"10.1038\/modpathol.2013.122","volume":"27","author":"R Kanagal-Shamanna","year":"2014","unstructured":"Kanagal-Shamanna R, Portier BP, Singh RR, Routbort MJ, Aldape KD, Handal BA, Rahimi H, Reddy NG, Barkoh BA, Mishra BM, Paladugu AV, Manekia JH, Kalhor N, Chowdhuri SR, Staerkel GA, Medeiros LJ, Luthra R, Patel KP (2014) Next-generation sequencing-based multi-gene mutation profiling of solid tumors using fine needle aspiration samples: promises and challenges for routine clinical diagnostics. Mod Pathol 27:314\u2013327","journal-title":"Mod Pathol"},{"key":"2559_CR22","doi-asserted-by":"publisher","first-page":"553","DOI":"10.1038\/bjc.2016.452","volume":"F116","author":"SO Hynes","year":"2017","unstructured":"Hynes SO, Pang B, James JA, Maxwell P, Salto-Tellez M (2017) Tissue-based next-generation sequencing: application in a universal healthcare system. Br J Cancer F116:553\u2013560","journal-title":"Br J Cancer"},{"key":"2559_CR23","doi-asserted-by":"publisher","first-page":"3070","DOI":"10.1093\/annonc\/mdx528","volume":"28","author":"DL Velden van der","year":"2017","unstructured":"van der Velden DL, van Herpen CML, van Laarhoven HWM, Smit EF, Groen HJM, Willems SM, Nederlof PM, Langenberg MHG, Cuppen E, Sleijfer S, Steeghs N, Voest EE (2017) Molecular tumor boards: current practice and future needs. Ann Oncol 28:3070\u20133075","journal-title":"Ann Oncol"},{"key":"2559_CR24","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1016\/j.lungcan.2017.03.018","volume":"108","author":"F Kron","year":"2017","unstructured":"Kron F, Kostenko A, Scheffler M, M\u00fcller D, Glossmann JP, Fischer R, Michels S, Nogova L, Hallek M, Zander T, Wolf J (2017) Economic burden of clinical trials in lung cancer in a German Comprehensive Cancer Center. Lung Cancer 108:134\u2013139","journal-title":"Lung Cancer"},{"key":"2559_CR25","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1016\/j.jmoldx.2017.01.011","volume":"19","author":"LJ Jennings","year":"2017","unstructured":"Jennings LJ, Arcila ME, Corless C, Kamel-Reid S, Lubin IM, Pfeifer J, Temple-Smolkin RL, Voelkerding KV, Nikiforova MN (2017) Guidelines for validation of next-generation sequencing-based oncology panels: a joint consensus recommendation of the Association for Molecular Pathology and College of American Pathologists. J Mol Diagn 19:341\u2013365","journal-title":"J Mol Diagn"},{"key":"2559_CR26","doi-asserted-by":"crossref","unstructured":"Maxwell P, Hynes SO, Fuchs M, Craig S, McGready C, McLean F, McQuaid S, James J, Salto-Tellez M (2018) Practical guide for the comparison of two next-generation sequencing systems for solid tumour analysis in a universal healthcare system. J Clin Pathol","DOI":"10.1136\/jclinpath-2017-204917"},{"key":"2559_CR27","doi-asserted-by":"publisher","first-page":"684","DOI":"10.1634\/theoncologist.2016-0030","volume":"21","author":"JH Suh","year":"2016","unstructured":"Suh JH, Johnson A, Albacker L, Wang K, Chmielecki J, Frampton G, Gay L, Elvin JA, Vergilio JA, Ali S, Miller VA, Stephens PJ, Ross JS (2016) Comprehensive genomic profiling facilitates implementation of the National Comprehensive Cancer Network Guidelines for Lung Cancer biomarker testing and identifies patients who may benefit from enrollment in mechanism-driven clinical trials. Oncologist 21:684\u2013691","journal-title":"Oncologist"},{"key":"2559_CR28","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1016\/j.lungcan.2018.03.003","volume":"119","author":"SR Turner","year":"2018","unstructured":"Turner SR, Buonocore D, Desmeules P, Rekhtman N, Dogan S, Lin O, Arcila ME, Jones DR, Huang J (2018) Feasibility of endobronchial ultrasound transbronchial needle aspiration for massively parallel next-generation sequencing in thoracic cancer patients. Lung Cancer 119:85\u201390","journal-title":"Lung Cancer"},{"key":"2559_CR29","doi-asserted-by":"publisher","first-page":"802","DOI":"10.1038\/bjc.2017.8","volume":"116","author":"U Malapelle","year":"2017","unstructured":"Malapelle U, Mayo de Las-Casas C, Rocco D, Garzon M, Pisapia P, Jordana-Ariza N, Russo M, Sgariglia R, De Luca C, Pepe F, Martinez-Bueno A, Morales-Espinosa D, Gonz\u00e1lez-Cao M, Karachaliou N, Viteri Ramirez S, Bellevicine C, Molina-Vila MA, Rosell R, Troncone G (2017) Development of a gene panel for next-generation sequencing of clinically relevant mutations in cell-free DNA from cancer patients. Br J Cancer 116:802\u2013810","journal-title":"Br J Cancer"},{"key":"2559_CR30","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1002\/cncy.21361","volume":"122","author":"HE Karnes","year":"2014","unstructured":"Karnes HE, Duncavage EJ, Bernadt CT (2014) Targeted next-generation sequencing using fine-needle aspirates from adenocarcinomas of the lung. Cancer Cytopathol 122:104\u2013113","journal-title":"Cancer Cytopathol"},{"issue":"Suppl 13","key":"2559_CR31","doi-asserted-by":"publisher","first-page":"S1383","DOI":"10.21037\/jtd.2017.06.97","volume":"9","author":"P Pisapia","year":"2017","unstructured":"Pisapia P, Pepe F, Smeraglio R, Russo M, Rocco D, Sgariglia R, Nacchio M, De Luca C, Vigliar E, Bellevicine C, Troncone G, Malapelle U (2017) Cell free DNA analysis by SiRe(\u00ae) next generation sequencing panel in non-small cell lung cancer patients: focus on basal setting. J Thorac Dis 9(Suppl 13):S1383\u2013S1390","journal-title":"J Thorac Dis"},{"key":"2559_CR32","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1136\/jclinpath-2018-205386","volume":"72","author":"F Pepe","year":"2019","unstructured":"Pepe F, De Luca C, Smeraglio R, Pisapia P, Sgariglia R, Nacchio M, Russo M, Serra N, Rocco D, Battiloro C, Ambrosio F, Gragnano G, Vigliar E, Bellevicine C, Troncone G, Malapelle U (2019) Performance analysis of SiRe next-generation sequencing panel in diagnostic setting: focus on NSCLC routine samples. J Clin Pathol 72:38\u201345","journal-title":"J Clin Pathol"},{"key":"2559_CR33","doi-asserted-by":"publisher","first-page":"615","DOI":"10.1002\/cncy.21868","volume":"125","author":"U Malapelle","year":"2017","unstructured":"Malapelle U, Mayo-de-Las-Casas C, Molina-Vila MA, Rosell R, Savic S, Bihl M, Bubendorf L, Salto-Tellez M, de Biase D, Tallini G, Hwang DH, Sholl LM, Luthra R, Weynand B, Vander Borght S, Missiaglia E, Bongiovanni M, Stieber D, Vielh P, Schmitt F, Rappa A, Barberis M, Pepe F, Pisapia P, Serra N, Vigliar E, Bellevicine C, Fassan M, Rugge M, de Andrea CE, Lozano MD, Basolo F, Fontanini G, Nikiforov YE, Kamel-Reid S, da Cunha SG, Nikiforova MN, Roy-Chowdhuri S, Troncone G, Molecular Cytopathology Meeting Group (2017) Consistency and reproducibility of next-generation sequencing and other multigene mutational assays: a worldwide ring trial study on quantitative cytological molecular reference specimens. Cancer Cytopathol 125:615\u2013626","journal-title":"Cancer Cytopathol"},{"key":"2559_CR34","doi-asserted-by":"publisher","first-page":"1127","DOI":"10.5858\/arpa.2017-0444-RA","volume":"142","author":"D Jain","year":"2018","unstructured":"Jain D, Roy-Chowdhuri S (2018) Molecular pathology of lung cancer cytology specimens: a concise review. Arch Pathol Lab Med 142:1127\u20131133","journal-title":"Arch Pathol Lab Med"},{"key":"2559_CR35","doi-asserted-by":"publisher","first-page":"807","DOI":"10.6004\/jnccn.2018.0062","volume":"16","author":"DS Ettinger","year":"2018","unstructured":"Ettinger DS, Aisner DL, Wood DE, Akerley W, Bauman J, Chang JY, Chirieac LR, D\u2019Amico TA, Dilling TJ, Dobelbower M, Govindan R, Gubens MA, Hennon M, Horn L, Lackner RP, Lanuti M, Leal TA, Lilenbaum R, Lin J, Loo BW Jr, Martins R, Otterson GA, Patel SP, Reckamp K, Riely GJ, Schild SE, Shapiro TA, Stevenson J, Swanson SJ, Tauer K, Yang SC, Gregory K, Hughes M (2018) NCCN guidelines insights: non-small cell lung cancer, version 5.2018. J Natl Compr Cancer Netw 16:807\u2013821","journal-title":"J Natl Compr Cancer Netw"},{"key":"2559_CR36","doi-asserted-by":"publisher","first-page":"480","DOI":"10.5858\/arpa.2017-0135-RA","volume":"142","author":"G Al\u00ec","year":"2018","unstructured":"Al\u00ec G, Bruno R, Savino M, Giannini R, Pelliccioni S, Menghi M, Boldrini L, Proietti A, Chella A, Ribechini A, Fontanini G (2018) Analysis of fusion genes by NanoString system: a role in lung cytology? Arch Pathol Lab Med 142:480\u2013489","journal-title":"Arch Pathol Lab Med"},{"key":"2559_CR37","doi-asserted-by":"publisher","first-page":"803","DOI":"10.1136\/jclinpath-2017-204373","volume":"70","author":"R Sgariglia","year":"2017","unstructured":"Sgariglia R, Pisapia P, Nacchio M, De Luca C, Pepe F, Russo M, Bellevicine C, Troncone G, Malapelle U (2017) Multiplex digital colour-coded barcode technology on RNA extracted from routine cytological samples of patients with non-small cell lung cancer: pilot study. J Clin Pathol 70:803\u2013806","journal-title":"J Clin Pathol"},{"key":"2559_CR38","unstructured":"Cancer Genome Atlas Research Network (2017) Electronic address: andrew_aguirre@dfci.harvard.edu; Cancer Genome Atlas Research Network. Integrated Genomic Characterization of Pancreatic Ductal Adenocarcinoma. Cancer Cell 32:185\u2013203.e13"},{"key":"2559_CR39","unstructured":"Pancreas and ampullary region, in Rosai and Ackerman\u2019s Surgical Pathology 11th edition, by Goldblum, Lamps, McKenney and Myers (2018) Elsevier, pp 886\u2013933"},{"key":"2559_CR40","doi-asserted-by":"publisher","first-page":"323","DOI":"10.3233\/CBM-170464","volume":"21","author":"M Masetti","year":"2018","unstructured":"Masetti M, Acquaviva G, Visani M, Tallini G, Fornelli A, Ragazzi M, Vasuri F, Grifoni D, Di Giacomo S, Fiorino S, Lombardi R, Tuminati D, Ravaioli M, Fabbri C, Bacchi-Reggiani ML, Pession A, Jovine E, de Biase D (2018) Long-term survivors of pancreatic adenocarcinoma show low rates of genetic alterations in KRAS, TP53 and SMAD4. Cancer Biomark 21:323\u2013334","journal-title":"Cancer Biomark"},{"key":"2559_CR41","doi-asserted-by":"publisher","first-page":"5018","DOI":"10.1158\/1078-0432.CCR-18-2645","volume":"24","author":"MJ Pishvaian","year":"2018","unstructured":"Pishvaian MJ, Bender RJ, Halverson D, Rahib L, Hendifar AE, Mikhail S, Chung V, Picozzi VJ, Sohal D, Blais EM, Mason K, Lyons EE, Matrisian LM, Brody JR, Madhavan S, Petricoin EF 3rd (2018) Molecular profiling of patients with pancreatic cancer: initial results from the know your tumor initiative. Clin Cancer Res 24:5018\u20135027","journal-title":"Clin Cancer Res"},{"key":"2559_CR42","unstructured":"de Biase D, de Luca C, Gragnano G, Visani M, Bellevicine C, Malapelle U, Tallini G, Troncone G (2016) Fully automated PCR detection of KRAS mutations on pancreatic endoscopic ultrasound fine-needle aspirates. J Clin Pathol doi: 10.1136"},{"key":"2559_CR43","doi-asserted-by":"publisher","first-page":"657","DOI":"10.1111\/den.12845","volume":"29","author":"C Fabbri","year":"2017","unstructured":"Fabbri C, Gibiino G, Fornelli A, Cennamo V, Grifoni D, Visani M, Acquaviva G, Fassan M, Fiorino S, Giovanelli S, Bassi M, Ghersi S, Tallini G, Jovine E, Gasbarrini A, de Biase D (2017) Team work and cytopathology molecular diagnosis of solid pancreatic lesions. Dig Endosc 29:657\u2013666","journal-title":"Dig Endosc"},{"key":"2559_CR44","doi-asserted-by":"publisher","first-page":"458","DOI":"10.5858\/arpa.2017-0215-RA","volume":"142","author":"D Biase de","year":"2018","unstructured":"de Biase D, Visani M, Acquaviva G, Fornelli A, Masetti M, Fabbri C, Pession A, Tallini G (2018) The role of next-generation sequencing in the cytologic diagnosis of pancreatic lesions. Arch Pathol Lab Med 142:458\u2013464","journal-title":"Arch Pathol Lab Med"},{"key":"2559_CR45","doi-asserted-by":"publisher","first-page":"688","DOI":"10.1002\/cncy.21338","volume":"121","author":"G Young","year":"2013","unstructured":"Young G, Wang K, He J, Otto G, Hawryluk M, Zwirco Z, Brennan T, Nahas M, Donahue A, Yelensky R, Lipson D, Sheehan CE, Boguniewicz AB, Stephens PJ, Miller VA, Ross JS (2013) Clinical next-generation sequencing successfully applied to fine-needle aspirations of pulmonary and pancreatic neoplasms. Cancer Cytopathol 121:688\u2013694","journal-title":"Cancer Cytopathol"},{"key":"2559_CR46","doi-asserted-by":"publisher","first-page":"546","DOI":"10.1177\/1066896912475073","volume":"21","author":"M Visani","year":"2013","unstructured":"Visani M, de Biase D, Baccarini P, Fabbri C, Polifemo AM, Zanini N, Pession A, Tallini G (2013) Multiple KRAS mutations in pancreatic adenocarcinoma: molecular features of neoplastic clones indicate the selection of divergent populations of tumor cells. Int J Surg Pathol 21:546\u2013552","journal-title":"Int J Surg Pathol"},{"key":"2559_CR47","doi-asserted-by":"publisher","first-page":"e87651","DOI":"10.1371\/journal.pone.0087651","volume":"9","author":"D Biase de","year":"2014","unstructured":"de Biase D, Visani M, Baccarini P, Polifemo AM, Maimone A, Fornelli A, Giuliani A, Zanini N, Fabbri C, Pession A, Tallini G (2014) Next generation sequencing improves the accuracy of KRAS mutation analysis in endoscopic ultrasound fine needle aspiration pancreatic lesions. PLoS One 9:e87651. \n                    https:\/\/doi.org\/10.1371\/journal.pone.0087651","journal-title":"PLoS One"},{"key":"2559_CR48","doi-asserted-by":"publisher","first-page":"7479","DOI":"10.3892\/mmr.2015.4344","volume":"12","author":"M Marco Di","year":"2015","unstructured":"Di Marco M, Astolfi A, Grassi E, Vecchiarelli S, Macchini M, Indio V, Casadei R, Ricci C, D\u2019Ambra M, Taffurelli G, Serra C, Ercolani G, Santini D, D\u2019Errico A, Pinna AD, Minni F, Durante S, Martella LR, Biasco G (2015) Characterization of pancreatic ductal adenocarcinoma using whole transcriptome sequencing and copy number analysis by single-nucleotide polymorphism array. Mol Med Rep 12:7479\u20137484","journal-title":"Mol Med Rep"},{"key":"2559_CR49","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1007\/s00535-014-0954-y","volume":"50","author":"Y Kubota","year":"2015","unstructured":"Kubota Y, Kawakami H, Natsuizaka M, Kawakubo K, Marukawa K, Kudo T, Abe Y, Kubo K, Kuwatani M, Hatanaka Y, Mitsuhashi T, Matsuno Y, Sakamoto N (2015) CTNNB1 mutational analysis of solid-pseudopapillary neoplasms of the pancreas using endoscopic ultrasound-guided fine-needle aspiration and next-generation deep sequencing. J Gastroenterol 50:203\u2013210","journal-title":"J Gastroenterol"},{"key":"2559_CR50","doi-asserted-by":"publisher","first-page":"124","DOI":"10.1016\/j.jmoldx.2015.08.002","volume":"18","author":"JC Dudley","year":"2016","unstructured":"Dudley JC, Zheng Z, McDonald T, Le LP, Dias-Santagata D, Borger D, Batten J, Vernovsky K, Sweeney B, Arpin RN, Brugge WR, Forcione DG, Pitman MB, Iafrate AJ (2016) Next-generation sequencing and fluorescence in situ hybridization have comparable performance characteristics in the analysis of pancreaticobiliary brushings for malignancy. J Mol Diagn 18:124\u2013130","journal-title":"J Mol Diagn"},{"key":"2559_CR51","doi-asserted-by":"publisher","first-page":"3875","DOI":"10.3892\/ol.2016.5168","volume":"12","author":"E Kameta","year":"2016","unstructured":"Kameta E, Sugimori K, Kaneko T, Ishii T, Miwa H, Sato T, Ishii Y, Sue S, Sasaki T, Yamashita Y, Shibata W, Matsumoto N, Maeda S (2016) Diagnosis of pancreatic lesions collected by endoscopic ultrasound-guided fine-needle aspiration using next-generation sequencing. Oncol Lett 12:3875\u20133881","journal-title":"Oncol Lett"},{"key":"2559_CR52","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1097\/SLA.0000000000001156","volume":"263","author":"V Valero 3rd","year":"2016","unstructured":"Valero V 3rd, Saunders TJ, He J, Weiss MJ, Cameron JL, Dholakia A, Wild AT, Shin EJ, Khashab MA, O\u2019Broin-Lennon AM, Ali SZ, Laheru D, Hruban RH, Iacobuzio-Donahue CA, Herman JM, Wolfgang CL (2016) Reliable detection of somatic mutations in fine needle aspirates of pancreatic cancer with next-generation sequencing: implications for surgical management. Ann Surg 263:153\u2013161","journal-title":"Ann Surg"},{"key":"2559_CR53","doi-asserted-by":"publisher","first-page":"54526","DOI":"10.18632\/oncotarget.9440","volume":"7","author":"FC Gleeson","year":"2016","unstructured":"Gleeson FC, Kerr SE, Kipp BR, Voss JS, Minot DM, Tu ZJ, Henry MR, Graham RP, Vasmatzis G, Cheville JC, Lazaridis KN, Levy MJ (2016) Targeted next generation sequencing of endoscopic ultrasound acquired cytology from ampullary and pancreatic adenocarcinoma has the potential to aid patient stratification for optimal therapy selection. Oncotarget 7:54526\u201354536. \n                    https:\/\/doi.org\/10.18632\/oncotarget.9440","journal-title":"Oncotarget"},{"key":"2559_CR54","doi-asserted-by":"publisher","first-page":"174","DOI":"10.1136\/jclinpath-2016-203928","volume":"70","author":"BG Sibinga Mulder","year":"2017","unstructured":"Sibinga Mulder BG, Mieog JS, Handgraaf HJ, Farina Sarasqueta A, Vasen HF, Potjer TP, Swijnenburg RJ, Luelmo SA, Feshtali S, Inderson A, Vahrmeijer AL, Bonsing BA, van Wezel T, Morreau H (2017) Targeted next-generation sequencing of FNA-derived DNA in pancreatic cancer. J Clin Pathol 70:174\u2013178","journal-title":"J Clin Pathol"},{"key":"2559_CR55","doi-asserted-by":"publisher","first-page":"246","DOI":"10.1136\/jclinpath-2017-204607","volume":"71","author":"BG Sibinga Mulder","year":"2018","unstructured":"Sibinga Mulder BG, Mieog JSD, Farina Sarasqueta A, Handgraaf HJ, Vasen HFA, Swijnenburg RJ, Luelmo SAC, Feshtali S, Inderson A, Vahrmeijer AL, Bonsing BA, Wezel TV, Morreau H (2018) Diagnostic value of targeted next-generation sequencing in patients with suspected pancreatic or periampullary cancer. J Clin Pathol 71:246\u2013252","journal-title":"J Clin Pathol"},{"key":"2559_CR56","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1002\/path.4344","volume":"233","author":"E Amato","year":"2014","unstructured":"Amato E, Molin MD, Mafficini A, Yu J, Malleo G, Rusev B, Fassan M, Antonello D, Sadakari Y, Castelli P, Zamboni G, Maitra A, Salvia R, Hruban RH, Bassi C, Capelli P, Lawlor RT, Goggins M, Scarpa A (2014) Targeted next-generation sequencing of cancer genes dissects the molecular profiles of intraductal papillary neoplasms of the pancreas. J Pathol 233:217\u2013227","journal-title":"J Pathol"},{"key":"2559_CR57","doi-asserted-by":"publisher","first-page":"404","DOI":"10.1016\/j.canlet.2014.09.029","volume":"356","author":"J Wang","year":"2015","unstructured":"Wang J, Paris PL, Chen J, Ngo V, Yao H, Frazier ML, Killary AM, Liu CG, Liang H, Mathy C, Bondada S, Kirkwood K, Sen S (2015) Next generation sequencing of pancreatic cyst fluid microRNAs from low grade-benign and high grade-invasive lesions. Cancer Lett 356:404\u2013409","journal-title":"Cancer Lett"},{"key":"2559_CR58","doi-asserted-by":"publisher","first-page":"1501","DOI":"10.1053\/j.gastro.2015.07.041","volume":"149","author":"S Springer","year":"2015","unstructured":"Springer S, Wang Y, Dal Molin M, Masica DL, Jiao Y, Kinde I, Blackford A, Raman SP, Wolfgang CL, Tomita T, Niknafs N, Douville C, Ptak J, Dobbyn L, Allen PJ, Klimstra DS, Schattner MA, Schmidt CM, Yip-Schneider M, Cummings OW, Brand RE, Zeh HJ, Singhi AD, Scarpa A, Salvia R, Malleo G, Zamboni G, Falconi M, Jang JY, Kim SW, Kwon W, Hong SM, Song KB, Kim SC, Swan N, Murphy J, Geoghegan J, Brugge W, Fernandez-Del Castillo C, Mino-Kenudson M, Schulick R, Edil BH, Adsay V, Paulino J, van Hooft J, Yachida S, Nara S, Hiraoka N, Yamao K, Hijioka S, van der Merwe S, Goggins M, Canto MI, Ahuja N, Hirose K, Makary M, Weiss MJ, Cameron J, Pittman M, Eshleman JR, Diaz LA Jr, Papadopoulos N, Kinzler KW, Karchin R, Hruban RH, Vogelstein B, Lennon AM (2015) A combination of molecular markers and clinical features improve the classification of pancreatic cysts. Gastroenterology 149:1501\u20131510","journal-title":"Gastroenterology"},{"key":"2559_CR59","doi-asserted-by":"publisher","first-page":"140","DOI":"10.1016\/j.gie.2015.06.047","volume":"83","author":"M Jones","year":"2016","unstructured":"Jones M, Zheng Z, Wang J, Dudley J, Albanese E, Kadayifci A, Dias-Santagata D, Le L, Brugge WR, Fernandez-del Castillo C, Mino-Kenudson M, Iafrate AJ, Pitman MB (2016) Impact of next-generation sequencing on the clinical diagnosis of pancreatic cysts. Gastrointest Endosc 83:140\u2013148","journal-title":"Gastrointest Endosc"},{"key":"2559_CR60","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1002\/cncy.21775","volume":"125","author":"MW Rosenbaum","year":"2017","unstructured":"Rosenbaum MW, Jones M, Dudley JC, Le LP, Iafrate AJ, Pitman MB (2017) Next-generation sequencing adds value to the preoperative diagnosis of pancreatic cysts. Cancer Cytopathol 125:41\u201347","journal-title":"Cancer Cytopathol"},{"key":"2559_CR61","doi-asserted-by":"publisher","first-page":"2131","DOI":"10.1136\/gutjnl-2016-313586","volume":"67","author":"AD Singhi","year":"2018","unstructured":"Singhi AD, McGrath K, Brand RE, Khalid A, Zeh HJ, Chennat JS, Fasanella KE, Papachristou GI, Slivka A, Bartlett DL, Dasyam AK, Hogg M, Lee KK, Marsh JW, Monaco SE, Ohori NP, Pingpank JF, Tsung A, Zureikat AH, Wald AI, Nikiforova MN (2018) Preoperative next-generation sequencing of pancreatic cyst fluid is highly accurate in cyst classification and detection of advanced neoplasia. Gut 67:2131\u20132141","journal-title":"Gut"},{"key":"2559_CR62","doi-asserted-by":"publisher","first-page":"1040","DOI":"10.1158\/2159-8290.CD-15-0274","volume":"5","author":"OA Zill","year":"2015","unstructured":"Zill OA, Greene C, Sebisanovic D, Siew LM, Leng J, Vu M, Hendifar AE, Wang Z, Atreya CE, Kelley RK, Van Loon K, Ko AH, Tempero MA, Bivona TG, Munster PN, Talasaz A, Collisson EA (2015) Cell-free DNA next-generation sequencing in pancreatobiliary carcinomas. Cancer Discov 5:1040\u20131048","journal-title":"Cancer Discov"},{"key":"2559_CR63","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1053\/j.gastro.2016.04.034","volume":"151","author":"AW Berger","year":"2016","unstructured":"Berger AW, Schwerdel D, Costa IG, Hackert T, Strobel O, Lam S, Barth TF, Schr\u00f6ppel B, Meining A, B\u00fcchler MW, Zenke M, Hermann PC, Seufferlein T, Kleger A (2016) Detection of hot-spot mutations in circulating cell-free DNA from patients with intraductal papillary mucinous neoplasms of the pancreas. Gastroenterology 151:267\u2013270","journal-title":"Gastroenterology"},{"key":"2559_CR64","doi-asserted-by":"publisher","first-page":"1677","DOI":"10.1136\/gutjnl-2015-311166","volume":"66","author":"J Yu","year":"2017","unstructured":"Yu J, Sadakari Y, Shindo K, Suenaga M, Brant A, Almario JAN, Borges M, Barkley T, Fesharakizadeh S, Ford M, Hruban RH, Shin EJ, Lennon AM, Canto MI, Goggins M (2017) Digital next-generation sequencing identifies low-abundance mutations in pancreatic juice samples collected from the duodenum of patients with pancreatic cancer and intraductal papillary mucinous neoplasms. Gut 66:1677\u20131687","journal-title":"Gut"},{"key":"2559_CR65","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1159\/000468519","volume":"6","author":"R Paschke","year":"2017","unstructured":"Paschke R, Cantara S, Crescenzi A, Jarzab B, Musholt TJ, Sobrinho Simoes M (2017) European thyroid association guidelines regarding thyroid nodule molecular fine-needle aspiration cytology diagnostics. Eur Thyroid J 6:115\u2013129","journal-title":"Eur Thyroid J"},{"key":"2559_CR66","doi-asserted-by":"crossref","unstructured":"Steward DL, Carty SE, Sippel RS, Yang SP, Sosa JA, Sipos JA, Figge JJ, Mandel S, Haugen BR, Burman KD, Baloch ZW, Lloyd RV, Seethala RR, Gooding WE, Chiosea SI, Gomes-Lima C, Ferris RL, Folek JM, Khawaja RA, Kundra P, Loh KS, Marshall CB, Mayson S, McCoy KL, Nga ME, Ngiam KY, Nikiforova MN, Poehls JL, Ringel MD, Yang H, Yip L, Nikiforov YE (2018) Performance of a multigene genomic classifier in thyroid nodules with indeterminate cytology: a prospective blinded multicenter study. JAMA Oncol","DOI":"10.1001\/jamaoncol.2018.4616"},{"key":"2559_CR67","doi-asserted-by":"publisher","first-page":"776","DOI":"10.1002\/cncy.21780","volume":"124","author":"C Bellevicine","year":"2016","unstructured":"Bellevicine C, Sgariglia R, Malapelle U, Vigliar E, Nacchio M, Ciancia G, Eszlinger M, Paschke R, Troncone G (2016) Young investigator challenge: can the ion AmpliSeq Cancer Hotspot Panel v2 be used for next-generation sequencing of thyroid FNA samples? Cancer Cytopathol 124:776\u2013784","journal-title":"Cancer Cytopathol"},{"key":"2559_CR68","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1002\/cncy.21522","volume":"123","author":"RH Shah","year":"2015","unstructured":"Shah RH, Scott SN, Brannon AR, Levine DA, Lin O, Berger MF (2015) Comprehensive mutation profiling by next-generation sequencing of effusion fluids from patients with high-grade serous ovarian carcinoma. Cancer Cytopathol 123:289\u2013297","journal-title":"Cancer Cytopathol"}],"container-title":["Virchows Archiv"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00428-019-02559-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00428-019-02559-z\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00428-019-02559-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,3,14]],"date-time":"2020-03-14T00:10:17Z","timestamp":1584144617000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00428-019-02559-z"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,15]]},"references-count":68,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,7]]}},"alternative-id":["2559"],"URL":"https:\/\/doi.org\/10.1007\/s00428-019-02559-z","relation":{},"ISSN":["0945-6317","1432-2307"],"issn-type":[{"value":"0945-6317","type":"print"},{"value":"1432-2307","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3,15]]},"assertion":[{"value":"20 February 2019","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 February 2019","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 March 2019","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 March 2019","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and\/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. For this review, formal consent is not required.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"The authors declare that they have no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}