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This study introduces two innovative silica-based nanosensors that offer real-time, on-site detection of GHB in spiked beverages at concentrations typical of chemical submission cases. Both chemosensors are based on silica nanoparticles functionalized with 2-aminonaphtoxazole derivatives. The first nanosensor, modified with a <jats:italic>p<\/jats:italic>-nitroaniline chromogenic unit, enables a visible color change for immediate visual identification, while the second incorporates a fluorescein derivative for high-sensitivity detection via fluorescence. Both nanosystems demonstrate exceptional specificity to GHB, with minimal interference from other substances. Moreover, the solid-phase integration of these nanosensors onto hydrophobic cellulose-based substrates enhances their portability and user-friendliness, making them suitable for nightlife venues and forensic applications. Finally, chromogenic precursor <jats:bold>1<\/jats:bold> has proven to be an excellent chemosensor for the visual detection of GHB in oral fluid, with a calculated LOD of 19.2 \u03bcM, and a linear response in the 32\u2013132 \u03bcM range.<\/jats:p>\n          <jats:p>\n            <jats:bold>Graphical abstract<\/jats:bold>\n          <\/jats:p>","DOI":"10.1007\/s00604-025-07134-9","type":"journal-article","created":{"date-parts":[[2025,4,4]],"date-time":"2025-04-04T13:25:00Z","timestamp":1743773100000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Silica-based optical chemosensors for rapid and reliable on-site detection of gamma-hydroxybutyrate in beverages and oral fluids"],"prefix":"10.1007","volume":"192","author":[{"given":"Jordi","family":"Hern\u00e1ndez-Contreras","sequence":"first","affiliation":[]},{"given":"Jordi","family":"Roig-Rubio","sequence":"additional","affiliation":[]},{"given":"Margarita","family":"Parra","sequence":"additional","affiliation":[]},{"given":"Salvador","family":"Gil","sequence":"additional","affiliation":[]},{"given":"Pau","family":"Arroyo","sequence":"additional","affiliation":[]},{"given":"Jos\u00e9 A.","family":"S\u00e1ez","sequence":"additional","affiliation":[]},{"given":"Carlos","family":"Lodeiro","sequence":"additional","affiliation":[]},{"given":"Pablo","family":"Gavi\u00f1a","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2025,4,2]]},"reference":[{"key":"7134_CR1","doi-asserted-by":"publisher","first-page":"529","DOI":"10.1016\/B978-0-12-800212-4.00049-2","volume-title":"Neuropathology of drug addictions and substance misuse","author":"W Xu","year":"2016","unstructured":"Xu W, Zhai D, Chang YT (2016) Detection of GHB by optical methods. 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