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In order to streamline laboratory workflow, the use of the bacterial suspension obtained through FASTinov\u00ae sample <jats:italic>prep<\/jats:italic> was tested for identification (ID) by matrix absorption laser deionization\u2013time of flight mass spectrometry (MALDI-TOF MS) (Bruker) in 364 positive BC, and its accuracy assessed comparing with the MALDI-TOF MS ID of the next-day subcultured colonies. FASTinov sample\u00a0<jats:italic>prep\u00a0<\/jats:italic>was highly reliable for rapid ID directly from BC with proportion of agreement of 94.9% for Gram-positive and 96.3% for Gram-negative bacteria.<\/jats:p>","DOI":"10.1007\/s10096-023-04725-3","type":"journal-article","created":{"date-parts":[[2023,12,19]],"date-time":"2023-12-19T11:02:46Z","timestamp":1702983766000},"page":"605-610","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["An improved protocol for bacteria identification by MALDI-TOF MS directly from positive blood cultures"],"prefix":"10.1007","volume":"43","author":[{"given":"Sara","family":"Cruz","sequence":"first","affiliation":[]},{"given":"David","family":"Abreu","sequence":"additional","affiliation":[]},{"given":"Ros\u00e1rio","family":"Gomes","sequence":"additional","affiliation":[]},{"given":"In\u00eas","family":"Martins-Oliveira","sequence":"additional","affiliation":[]},{"given":"Ana","family":"Silva-Dias","sequence":"additional","affiliation":[]},{"given":"Blanca","family":"Perez-Viso","sequence":"additional","affiliation":[]},{"given":"Rafael","family":"Cant\u00f3n","sequence":"additional","affiliation":[]},{"given":"Cid\u00e1lia","family":"Pina-Vaz","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2023,12,19]]},"reference":[{"issue":"26","key":"4725_CR1","doi-asserted-by":"publisher","first-page":"142","DOI":"10.1016\/j.cmi.2019.11.017","volume":"1","author":"B Lamy","year":"2020","unstructured":"Lamy B, Sundqvist M, Idelevich EA (2020) Bloodstream infections \u2013 standard and progress in pathogen diagnostics. 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