{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T02:57:31Z","timestamp":1768273051873,"version":"3.49.0"},"reference-count":47,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2013,5,1]],"date-time":"2013-05-01T00:00:00Z","timestamp":1367366400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>There is a growing demand for natural colorants. This is prompting the search for new alternative and \u201cbenign\u201d separation systems allowing higher recoveries, extraction yields, and selectivities. This work investigates the use of aqueous two-phase systems (ATPS) based on ionic liquids as extraction processes for the recovery of red colorants from the fermented broth of Penicillium purpurogenum DPUA 1275. Several ATPS based on quaternary ammonium and imidazolium were studied in this work aiming at separating the red colorants produced from the remaining colorants and contaminant proteins present in the fermented broth. The results suggest that the red colorants can be isolated by an appropriate manipulation of some of the process conditions, such as the use of quaternary ammonium with short alkyl chains, alkaline media, and short tie-line lengths (extraction point systems with lower concentrations of ionic liquid). These conditions allow large partition coefficients for the red colorants (K \u00a0red\u00a0=\u00a024.4\u00a0\u00b1\u00a02.3), high protein removal (60.7\u00a0\u00b1\u00a02.8\u00a0%) and selectivity parameters (S \u00a0red\/prot\u00a0=\u00a010.05).<\/jats:p>","DOI":"10.1007\/s10295-013-1237-y","type":"journal-article","created":{"date-parts":[[2013,3,2]],"date-time":"2013-03-02T05:46:05Z","timestamp":1362203165000},"page":"507-516","source":"Crossref","is-referenced-by-count":66,"title":["Isolation of natural red colorants from fermented broth using ionic liquid-based aqueous two-phase systems"],"prefix":"10.1093","volume":"40","author":[{"given":"S\u00f3nia P M","family":"Ventura","sequence":"first","affiliation":[{"name":"grid.7311.4 0000000123236065 CICECO, Departamento de Qu\u00edmica Universidade de Aveiro 3810-193 Aveiro Portugal"}]},{"given":"Val\u00e9ria C","family":"Santos-Ebinuma","sequence":"additional","affiliation":[{"name":"grid.11899.38 0000000419370722 Departamento de Tecnologia Bioqu\u00edmico-Farmac\u00eautica Universidade de S\u00e3o Paulo 05508000 S\u00e3o Paulo Brazil"}]},{"given":"Jorge F B","family":"Pereira","sequence":"additional","affiliation":[{"name":"grid.7311.4 0000000123236065 CICECO, Departamento de Qu\u00edmica Universidade de Aveiro 3810-193 Aveiro Portugal"}]},{"given":"Maria F S","family":"Teixeira","sequence":"additional","affiliation":[{"name":"grid.411181.c 0000000122210517 Universidade Federal do Amazonas Av. Gal. Rodrigo Oct\u00e1vio Jord\u00e3o Ramos, 3000 69.077-000 Manaus AM Brazil"}]},{"given":"Adalberto","family":"Pessoa","sequence":"additional","affiliation":[{"name":"grid.11899.38 0000000419370722 Departamento de Tecnologia Bioqu\u00edmico-Farmac\u00eautica Universidade de S\u00e3o Paulo 05508000 S\u00e3o Paulo Brazil"}]},{"given":"Jo\u00e3o A P","family":"Coutinho","sequence":"additional","affiliation":[{"name":"grid.7311.4 0000000123236065 CICECO, Departamento de Qu\u00edmica Universidade de Aveiro 3810-193 Aveiro Portugal"}]}],"member":"286","published-online":{"date-parts":[[2013,5,1]]},"reference":[{"key":"2023072107222832700_CR1","doi-asserted-by":"publisher","first-page":"346","DOI":"10.1016\/j.jbiosc.2009.10.003","article-title":"Effect of light on growth, intracellular and extracellular pigment production by five pigment-producing filamentous fungi in synthetic medium","volume":"109","author":"Velmurugan","year":"2010","journal-title":"J Biosci Bioeng"},{"key":"2023072107222832700_CR2","doi-asserted-by":"publisher","first-page":"300","DOI":"10.1016\/j.tibtech.2010.03.004","article-title":"Fungal polyketide azaphilone pigments as future natural food colorants?","volume":"28","author":"Mapari","year":"2010","journal-title":"Trends Biotechnol"},{"key":"2023072107222832700_CR3","doi-asserted-by":"crossref","first-page":"2671","DOI":"10.1128\/aem.63.7.2671-2678.1997","article-title":"Production and identification of N-glucosylrubropuntacmine and N-glucosylmonascorubramine from Monascus ruber and occurrence of electron donor-acceptor complexes in these red pigments","volume":"63","author":"Hajjaj","year":"1997","journal-title":"Appl Environ Microbiol"},{"key":"2023072107222832700_CR4","doi-asserted-by":"publisher","first-page":"7027","DOI":"10.1021\/jf062094n","article-title":"Colorimetric characterization for comparative analyses of fungal pigments and natural food colorants","volume":"54","author":"Mapari","year":"2006","journal-title":"J Agric Food Chem"},{"key":"2023072107222832700_CR5","first-page":"1","article-title":"Identification of potentially safe promising fungal cell factories for the production of polyketide natural food colorants using chemotaxonomic rationale","volume":"24","author":"Mapari","year":"2009","journal-title":"Microb Cell Fact"},{"key":"2023072107222832700_CR6","doi-asserted-by":"publisher","first-page":"1582","DOI":"10.1360\/982004-367","article-title":"Extraction of penicillin G by aqueous two-phase system of [Bmim]BF4\/NaH2PO4","volume":"50","author":"Liu","year":"2005","journal-title":"Chin Sci Bull"},{"key":"2023072107222832700_CR7","doi-asserted-by":"publisher","first-page":"6082","DOI":"10.1016\/j.biortech.2011.01.040","article-title":"Improvement of the production of a red pigment in Penicillium sp. 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