{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T08:22:27Z","timestamp":1772439747269,"version":"3.50.1"},"reference-count":28,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2013,8,1]],"date-time":"2013-08-01T00:00:00Z","timestamp":1375315200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Flow cytometry was used to assess \u03b2-carotene content, cell membrane permeability, cell size and granularity in Rhodotorula glutinis mutant 400A15 grown under different oxygen transfer coefficients (k \u00a0L \u00a0a) and carbon to nitrogen ratios (C\/N). A Doehlert distribution was used in order to select the best conditions that induced the highest carotenoids production. The highest \u03b2-carotene content (0.79\u00a0mg\u00a0g\u22121 DCW) at the lowest k \u00a0L \u00a0a and C\/N (5\u00a0\u00d7\u00a010\u22123\u00a0s\u22121 and 11.3 respectively). Under these conditions, the biomass concentration attained 18.60\u00a0g\u00a0L\u22121. The highest ratio of cells with permeabilised membranes (2.6\u00a0%), and the highest cell size and granularity were also obtained under these conditions. It was observed that C\/N showed a stronger influence than the k \u00a0L \u00a0a on the measured cell parameters.<\/jats:p>","DOI":"10.1007\/s10295-013-1278-2","type":"journal-article","created":{"date-parts":[[2013,5,9]],"date-time":"2013-05-09T11:53:59Z","timestamp":1368100439000},"page":"865-875","source":"Crossref","is-referenced-by-count":16,"title":["Assessment of \u03b2-carotene content, cell physiology and morphology of the yellow yeast <i>Rhodotorula glutinis<\/i> mutant 400A15 using flow cytometry"],"prefix":"10.1093","volume":"40","author":[{"given":"Raffaela","family":"Cutzu","sequence":"first","affiliation":[{"name":"grid.11450.31 0000000120979138 Dipartamento di Agraria Universit\u00e0 degli Studi di Sassari Viale Italia 39 07100 Sassari Italy"}]},{"given":"Ana","family":"Clemente","sequence":"additional","affiliation":[{"name":"grid.420766.1 0000000121063068 Laborat\u00f3rio Nacional de Energia e Geologia, Unidade de Bioenergia Estrada do Pa\u00e7o do Lumiar, 22 1649-038 Lisbon Portugal"}]},{"given":"Alberto","family":"Reis","sequence":"additional","affiliation":[{"name":"grid.420766.1 0000000121063068 Laborat\u00f3rio Nacional de Energia e Geologia, Unidade de Bioenergia Estrada do Pa\u00e7o do Lumiar, 22 1649-038 Lisbon Portugal"}]},{"given":"Beatriz","family":"Nobre","sequence":"additional","affiliation":[{"name":"grid.420766.1 0000000121063068 Laborat\u00f3rio Nacional de Energia e Geologia, Unidade de Bioenergia Estrada do Pa\u00e7o do Lumiar, 22 1649-038 Lisbon Portugal"}]},{"given":"Ilaria","family":"Mannazzu","sequence":"additional","affiliation":[{"name":"grid.11450.31 0000000120979138 Dipartamento di Agraria Universit\u00e0 degli Studi di Sassari Viale Italia 39 07100 Sassari Italy"}]},{"given":"Jos\u00e9","family":"Roseiro","sequence":"additional","affiliation":[{"name":"grid.420766.1 0000000121063068 Laborat\u00f3rio Nacional de Energia e Geologia, Unidade de Bioenergia Estrada do Pa\u00e7o do Lumiar, 22 1649-038 Lisbon Portugal"}]},{"given":"Teresa","family":"Lopes da Silva","sequence":"additional","affiliation":[{"name":"grid.420766.1 0000000121063068 Laborat\u00f3rio Nacional de Energia e Geologia, Unidade de Bioenergia Estrada do Pa\u00e7o do Lumiar, 22 1649-038 Lisbon Portugal"}]}],"member":"286","published-online":{"date-parts":[[2013,8,1]]},"reference":[{"key":"2021033103084879300_CR1","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1016\/j.bej.2007.01.004","article-title":"Production of carotenoids by the isolated yeast of Rhodotorula glutinis","volume":"35","author":"Aksu","year":"2007","journal-title":"Biochem Eng J"},{"key":"2021033103084879300_CR2","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1038\/nbt0191-70","article-title":"Isolation and characterization of carotenoid hyperproducing mutants of yeast by flow cytometry and cell sorting","volume":"9","author":"An","year":"1991","journal-title":"Bio\/Technol"},{"key":"2021033103084879300_CR3","doi-asserted-by":"publisher","first-page":"1175","DOI":"10.1007\/s11274-011-0920-2","article-title":"Monitoring Rhodosporidium toruloides NCYC 921 batch fermentations growing under carbon and nitrogen limitation by flow Cytometry","volume":"28","author":"Andrade","year":"2012","journal-title":"World J Microbiol Biotechnol"},{"key":"2021033103084879300_CR4","doi-asserted-by":"publisher","first-page":"2169","DOI":"10.1351\/pac199769102169","article-title":"Commercial opprotunities for carotenoid production by biotechnology","volume":"69","author":"Ausin","year":"1997","journal-title":"Pure Appl Chem"},{"key":"2021033103084879300_CR5","first-page":"3999","article-title":"Antioxidant effects of carotenoids","volume":"68","author":"Bast","year":"1998","journal-title":"Inst J Vitamin Nutr Res"},{"key":"2021033103084879300_CR6","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1016\/S0960-8524(00)00075-4","article-title":"Production of \u03b2\u2013carotene by a Rhodotorula glutinis mutant in sea water","volume":"76","author":"Bhosale","year":"2001","journal-title":"Biores Technol"},{"key":"2021033103084879300_CR7","first-page":"23","article-title":"The impact of fluid mechanical stress in Saccharomyces cerevisiae cells during continuous cultivation in an agitated, aerated bioreactor; its implication for mixing in the brewing process and aerobic fermentations","volume":"81","author":"Boswell","year":"2003","journal-title":"Tran I Chem"},{"key":"2021033103084879300_CR8","doi-asserted-by":"publisher","first-page":"569","DOI":"10.1126\/science.6710156","article-title":"\u03b2-Carotene: an unusual type of lipid antioxidant","volume":"22","author":"Burton","year":"1984","journal-title":"Science"},{"key":"2021033103084879300_CR9"},{"key":"2021033103084879300_CR10","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1128\/MR.60.4.641-696.1996","article-title":"Flow cytometry and cell sorting of heterogeneous microbial populations-the importance of single-cell analyses","volume":"60","author":"Davey","year":"1996","journal-title":"Microbiol Rev"},{"key":"2021033103084879300_CR11","volume-title":"Experimental design: a chemometric approach","author":"Deming","year":"1987"},{"key":"2021033103084879300_CR12","doi-asserted-by":"publisher","first-page":"231","DOI":"10.2307\/2346327","article-title":"Uniform shell designs","volume":"19","author":"Doehlert","year":"1970","journal-title":"Appl Stat"},{"key":"2021033103084879300_CR13","doi-asserted-by":"publisher","first-page":"64","DOI":"10.4236\/fns.2012.31011","article-title":"Some factors affecting the production of carotenoids by Rhodotorula glutinis var. glutinis","volume":"3","author":"El-Banna","year":"2012","journal-title":"Food Nutr Sci"},{"key":"2021033103084879300_CR14","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1007\/s10295-008-0492-9","article-title":"Carotenoids from Rhodotorula and Phaffia: yeasts of biotechnology importance","volume":"36","author":"Frengova","year":"2009","journal-title":"J Ind Microbiol Biotechnol"},{"key":"2021033103084879300_CR15","first-page":"197","article-title":"The application of multiparameter flow cytometry to monitor individual microbial cell physiological state","volume":"89","author":"Hewitt","year":"2004","journal-title":"Adv Biochem Eng Biotechnol"},{"key":"2021033103084879300_CR16","doi-asserted-by":"publisher","first-page":"1686","DOI":"10.1002\/1097-0010(20000901)80:11&lt;1686::AID-JSFA694&gt;3.0.CO;2-Y","article-title":"Evaluation of free radical scavenging of dietary carotenoids by the stable radical 2,2-diphenyl-1-picrylhydrazyl","volume":"80","author":"Jimenez-Escrib","year":"2000","journal-title":"J Sci Food Agric"},{"key":"2021033103084879300_CR17","doi-asserted-by":"publisher","first-page":"2166","DOI":"10.1007\/s12010-010-8991-3","article-title":"Using multi-parameter flow cytometry to monitor the yeast Rhodotorula glutinis CCMI 145 batch growth and oil production towards biodiesel","volume":"162","author":"Lopes da Silva","year":"2010","journal-title":"Appl Biochem Biotechnol"},{"key":"2021033103084879300_CR18","doi-asserted-by":"publisher","first-page":"2998","DOI":"10.1016\/j.biortech.2010.10.008","article-title":"Monitoring Rhodotorula glutinis CCMI 145 physiological response and oil production growing on xylose and glucose using multi-parameter flow Cytometry","volume":"102","author":"Lopes da Silva","year":"2011","journal-title":"Biores Technol"},{"key":"2021033103084879300_CR19","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1016\/j.ijfoodmicro.2007.11.003","article-title":"Behaviour of Saccharomyces cerevisiae wine strains during adaptation to unfavourable condition of fermentation on synthetic medium: cell lipid composition, membrane integrity, viability and fermentative activity","volume":"121","author":"Mannazzu","year":"2008","journal-title":"Int J Food Microbiol"},{"key":"2021033103084879300_CR20"},{"key":"2021033103084879300_CR21","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1111\/j.1365-2672.1991.tb02922.x","article-title":"Microbial sources of carotenoid pigments used in foods and feeds","volume":"70","author":"Nelis","year":"1991","journal-title":"J Appl Bacteriol"},{"key":"2021033103084879300_CR22","doi-asserted-by":"publisher","first-page":"148","DOI":"10.1016\/j.seppur.2006.06.026","article-title":"Chemical disruption of yeast cells for the isolation of carotenoid pigments","volume":"53","author":"Park","year":"2007","journal-title":"Sep Purif Technol"},{"key":"2021033103084879300_CR23","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1007\/BF02529968","article-title":"Fatty acid and carotenoid composition of Rhodotorula strains","volume":"164","author":"Perrier","year":"1995","journal-title":"Arch Microbiol"},{"key":"2021033103084879300_CR24","doi-asserted-by":"publisher","first-page":"210","DOI":"10.1016\/j.procbio.2010.08.009","article-title":"Potential use of oleaginous red yeast Rhodotorula glutinis for the bioconversion of crude glycerol from biodiesel plant to lipids and carotenoids","volume":"46","author":"Saenge","year":"2011","journal-title":"Proc Biochem"},{"key":"2021033103084879300_CR25","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1515\/znc-2003-3-415","article-title":"Effect of aeration on the production of carotenoid pigments by Rhodotorula rubra-lactobacillus casei subsp. casei co-cultures in whey ultrafiltrate","volume":"58","author":"Simova","year":"2003","journal-title":"Z Naturforsch C"},{"key":"2021033103084879300_CR26","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1023\/A:1008917612616","article-title":"Inverse relationship between carotenoid and lipid formation in Rhodotorula gracilis according to the C\/N ratio and the growth medium","volume":"16","author":"Somashekar","year":"2000","journal-title":"World J Microbiol Biotechnol"},{"key":"2021033103084879300_CR27","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1111\/j.1574-6968.2008.01278.x","article-title":"Efficient screenning for astaxanthin-overproduction mutants of the yeast Xanthophyllomyces dendrorhous by flow cytometry","volume":"286","author":"Ukibe","year":"2008","journal-title":"FEMS Microbiol Lett"},{"key":"2021033103084879300_CR28","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1099\/00221287-5-1-167","article-title":"The measurement of the aeration of culture media","volume":"5","author":"Wise","year":"1951","journal-title":"J Gen Microbial"}],"container-title":["Journal of Industrial Microbiology and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10295-013-1278-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s10295-013-1278-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/academic.oup.com\/jimb\/article-pdf\/40\/8\/865\/36803230\/jimb0865.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/jimb\/article-pdf\/40\/8\/865\/36803230\/jimb0865.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T11:43:16Z","timestamp":1676374996000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimb\/article\/40\/8\/865\/5994678"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,8,1]]},"references-count":28,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2013,8,1]]}},"URL":"https:\/\/doi.org\/10.1007\/s10295-013-1278-2","relation":{},"ISSN":["1476-5535","1367-5435"],"issn-type":[{"value":"1476-5535","type":"electronic"},{"value":"1367-5435","type":"print"}],"subject":[],"published-other":{"date-parts":[[2013,8,1]]},"published":{"date-parts":[[2013,8,1]]}}}