{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T22:29:40Z","timestamp":1761863380397},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2014,12,1]],"date-time":"2014-12-01T00:00:00Z","timestamp":1417392000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"},{"start":{"date-parts":[[2014,12,1]],"date-time":"2014-12-01T00:00:00Z","timestamp":1417392000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The presence of toxic compounds derived from biomass pre-treatment in fermentation media represents an important drawback in second-generation bio-ethanol production technology and overcoming this inhibitory effect is one of the fundamental challenges to its industrial production. The aim of this study was to systematically identify, in industrial medium and at a genomic scale, the Saccharomyces cerevisiae genes required for simultaneous and maximal tolerance to key inhibitors of lignocellulosic fermentations. Based on the screening of EUROSCARF haploid mutant collection, 242 and 216 determinants of tolerance to inhibitory compounds present in industrial wheat straw hydrolysate (WSH) and in inhibitor-supplemented synthetic hydrolysate were identified, respectively. Genes associated to vitamin metabolism, mitochondrial and peroxisomal functions, ribosome biogenesis and microtubule biogenesis and dynamics are among the newly found determinants of WSH resistance. Moreover, PRS3, VMA8, ERG2, RAV1 and RPB4 were confirmed as key genes on yeast tolerance and fermentation of industrial WSH.<\/jats:p>","DOI":"10.1007\/s10295-014-1519-z","type":"journal-article","created":{"date-parts":[[2014,10,6]],"date-time":"2014-10-06T05:37:45Z","timestamp":1412573865000},"page":"1753-1761","update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Genome-wide screening of <i>Saccharomyces cerevisiae<\/i> genes required to foster tolerance towards industrial wheat straw hydrolysates"],"prefix":"10.1093","volume":"41","author":[{"given":"Francisco B","family":"Pereira","sequence":"first","affiliation":[{"name":"grid.10328.38 000000012159175X CEB-Centre of Biological Engineering Universidade do Minho Campus de Gualtar 4710-057 Braga Portugal"}]},{"given":"Miguel C","family":"Teixeira","sequence":"additional","affiliation":[{"name":"grid.9983.b 0000000121814263 Department of Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior T\u00e9cnico, IBB-Institute for Biotechnology and Bioengineering Universidade de Lisboa Avenida Rovisco Pais 1049-001 Lisbon Portugal"}]},{"given":"Nuno P","family":"Mira","sequence":"additional","affiliation":[{"name":"grid.9983.b 0000000121814263 Department of Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior T\u00e9cnico, IBB-Institute for Biotechnology and Bioengineering Universidade de Lisboa Avenida Rovisco Pais 1049-001 Lisbon Portugal"}]},{"given":"Isabel","family":"S\u00e1-Correia","sequence":"additional","affiliation":[{"name":"grid.9983.b 0000000121814263 Department of Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior T\u00e9cnico, IBB-Institute for Biotechnology and Bioengineering Universidade de Lisboa Avenida Rovisco Pais 1049-001 Lisbon Portugal"}]},{"given":"Luc\u00edlia","family":"Domingues","sequence":"additional","affiliation":[{"name":"grid.10328.38 000000012159175X CEB-Centre of Biological Engineering Universidade do Minho Campus de Gualtar 4710-057 Braga Portugal"}]}],"member":"286","published-online":{"date-parts":[[2014,12,1]]},"reference":[{"key":"2021033103283942100_CR1","doi-asserted-by":"crossref","DOI":"10.1002\/bit.24938","article-title":"Evolutionary engineering of Saccharomyces cerevisiae for enhanced tolerance to hydrolysates of lignocellulosic biomass","author":"Almario","year":"2013","journal-title":"Biotechnol Bioeng"},{"key":"2021033103283942100_CR2","doi-asserted-by":"publisher","first-page":"340","DOI":"10.1002\/jctb.1676","article-title":"Increased tolerance and conversion of inhibitors in lignocellulosic hydrolysates by Saccharomyces cerevisiae","volume":"82","author":"Almeida","year":"2007","journal-title":"J Chem Technol 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