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The process involves two or three steps, being the selection step a crucial one. For the industrial implementation of this strategy, reactor stability in terms of both performance and microbial community presence has to be considered. A long-term operation of a sequencing batch reactor under feast\/famine conditions was performed along with microbial community identification\/quantification using FISH and DGGE. The community was found to be extremely dynamic, dominated by Alphaproteobacteria, with Paracoccus and Rhodobacter present, both PHA-storing microorganisms. 16S rRNA gene clone library further revealed that side populations\u2019 non-PHA accumulators were able to strive (Agrobacterium, Flavobacteria, and Brachymonas). Nevertheless, reactor performance in terms of PHA storage was stable during operation time. The monitoring of the MMC population evolution provided information on the relation between community structure and process operation.<\/jats:p>","DOI":"10.1007\/s10295-017-1951-y","type":"journal-article","created":{"date-parts":[[2017,5,11]],"date-time":"2017-05-11T20:37:38Z","timestamp":1494535058000},"page":"1215-1224","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Highly complex substrates lead to dynamic bacterial community for polyhydroxyalkanoates production"],"prefix":"10.1093","volume":"44","author":[{"given":"Diogo","family":"Queir\u00f3s","sequence":"first","affiliation":[{"name":"0000000123236065 grid.7311.4 Chemistry Department, CICECO-Aveiro Institute of Materials University of Aveiro Campus Universit\u00e1rio de Santiago 3810-193 Aveiro Portugal"}]},{"given":"Alexandre","family":"Fonseca","sequence":"additional","affiliation":[{"name":"0000000123236065 grid.7311.4 Chemistry Department, CICECO-Aveiro Institute of Materials University of Aveiro Campus Universit\u00e1rio de Santiago 3810-193 Aveiro Portugal"}]},{"given":"Simona","family":"Rossetti","sequence":"additional","affiliation":[{"name":"Water Research Institute C.N.R. 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