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Using a DNA-Seq and metabarcode sequencing integrated approach, the first catalogue of endosymbionts associated with\n                    <jats:italic>T. erytreae<\/jats:italic>\n                    from the Iberian Peninsula, South Africa and African Islands, was generated. The almost complete genome of two new bacteria, one facultative and one obligate, tentatively named Asaia-like endosymbiont of\n                    <jats:italic>T. erytreae<\/jats:italic>\n                    and Sodalis-like endosymbiont of\n                    <jats:italic>T. erytreae,<\/jats:italic>\n                    respectively, was assembled and annotated. The complete mitochondrial genomes of\n                    <jats:italic>T. erytreae<\/jats:italic>\n                    from the geographical areas studied were also assembled and phylogenetic analyses were performed, suggesting that\n                    <jats:italic>T. erytreae<\/jats:italic>\n                    populations currently present in the Iberian Peninsula and specimens analyzed from South Africa may have originated from a common ancestor. Similar results were obtained when the genetic distances between Sodalis-like endosymbiont of\n                    <jats:italic>T. erytreae<\/jats:italic>\n                    were taken into consideration, thus supporting the symbiont\u2013host codivergence which suggests that this bacterium is approaching to an obligate status. Finally, a new genetic marker of\n                    <jats:italic>T. erytreae,<\/jats:italic>\n                    an insertion in the mitochondrial tRNA-Ser gene, was identified only in some European samples, showing for the first time the existence of two mixed subpopulations of\n                    <jats:italic>T. erytreae<\/jats:italic>\n                    . The integrated DNA-Seq and metabarcode sequencing approach used in this study, besides generating a catalogue of\n                    <jats:italic>T. erytreae<\/jats:italic>\n                    endosymbionts, provided novel data on the sequence variability of bacterial and insect mitochondrial genomes from different geographic areas, highlighting the possible original sources of currently spreading\n                    <jats:italic>T. erytreae<\/jats:italic>\n                    populations may be more complex than previously reported.\n                  <\/jats:p>","DOI":"10.1007\/s10340-025-01945-8","type":"journal-article","created":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T10:21:25Z","timestamp":1754389285000},"page":"2383-2402","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Assessment of Trioza erytreae microbiome and mitochondrial genome variability by integrated high-throughput sequencing approach"],"prefix":"10.1007","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8412-3037","authenticated-orcid":false,"given":"Michela","family":"Chiumenti","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4790-474X","authenticated-orcid":false,"given":"Vittorio","family":"Nicoloso","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6012-3270","authenticated-orcid":false,"given":"Alberto","family":"Fereres","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2260-0600","authenticated-orcid":false,"given":"Jos\u00e9 Alberto","family":"Pereira","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9639-4558","authenticated-orcid":false,"given":"Hans J.","family":"Maree","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2226-0968","authenticated-orcid":false,"given":"Rachelle","family":"Bester","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0009-0003-6403-3944","authenticated-orcid":false,"given":"Bernard","family":"Reynaud","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5216-5542","authenticated-orcid":false,"given":"H\u00e9l\u00e8ne","family":"Delatte","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9853-366X","authenticated-orcid":false,"given":"Leandro","family":"Pe\u00f1a","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4954-989X","authenticated-orcid":false,"given":"Vicente","family":"Pall\u00e1s","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4229-6906","authenticated-orcid":false,"given":"Pedro","family":"Serra","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8981-1018","authenticated-orcid":false,"given":"Beatriz","family":"Navarro","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2822-704X","authenticated-orcid":false,"given":"Francesco","family":"Di Serio","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2025,8,5]]},"reference":[{"key":"1945_CR1","doi-asserted-by":"publisher","first-page":"440","DOI":"10.3390\/biology9120440","volume":"9","author":"H Ahn","year":"2020","unstructured":"Ahn H, Seol D, Cho S, Kim H, Kwak W (2020) Enhanced symbiotic characteristics in bacterial genomes with the disruption of rRNA operon. 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