{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T21:34:55Z","timestamp":1775252095510,"version":"3.50.1"},"reference-count":41,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2013,5,3]],"date-time":"2013-05-03T00:00:00Z","timestamp":1367539200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Aerobiologia"],"published-print":{"date-parts":[[2013,12]]},"DOI":"10.1007\/s10453-013-9300-8","type":"journal-article","created":{"date-parts":[[2013,5,2]],"date-time":"2013-05-02T17:37:02Z","timestamp":1367516222000},"page":"513-521","source":"Crossref","is-referenced-by-count":15,"title":["Analysis of the pollen allergen content of twelve olive cultivars grown in Portugal"],"prefix":"10.1007","volume":"29","author":[{"given":"H.","family":"Ribeiro","sequence":"first","affiliation":[]},{"given":"S.","family":"Morales","sequence":"additional","affiliation":[]},{"given":"C.","family":"Salmer\u00f3n","sequence":"additional","affiliation":[]},{"given":"A.","family":"Cruz","sequence":"additional","affiliation":[]},{"given":"L.","family":"Calado","sequence":"additional","affiliation":[]},{"given":"M. I.","family":"Rodr\u00edguez-Garc\u00eda","sequence":"additional","affiliation":[]},{"given":"J. D.","family":"Alch\u00e9","sequence":"additional","affiliation":[]},{"given":"I.","family":"Abreu","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2013,5,3]]},"reference":[{"key":"9300_CR1","first-page":"1","volume-title":"Current insights in pollen allergens","author":"JD Alch\u00e9","year":"2012","unstructured":"Alch\u00e9, J. D., Castro, A. C., Jim\u00e9nez-L\u00f3pez, J. C., Morales, S., Zafra, A., Florido, F., et al. (2012). Pollen allergenicity is highly dependent on the plant genetic background: The \u201ccultivar issue\u201d. In J. C. Jim\u00e9nez-L\u00f3pez (Ed.), Current insights in pollen allergens (pp. 1\u201326). Croatia: Intech Open Science."},{"key":"9300_CR2","first-page":"17","volume":"17","author":"JD Alch\u00e9","year":"2007","unstructured":"Alch\u00e9, J. D., Castro, A. J., Jimenez-Lopez, J. C., Morales, S., Zafra, A., Hamman-Khalifa, A. M., et al. (2007). Differential characteristics of olive pollen from different cultivars: Biological and clinical implications. Journal of Investigational Allergology and Clinical Immunology, 17, 17\u201323.","journal-title":"Journal of Investigational Allergology and Clinical Immunology"},{"issue":"4","key":"9300_CR3","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1034\/j.1399-3054.1998.1040437.x","volume":"104","author":"JD Alch\u00e9","year":"1998","unstructured":"Alch\u00e9, J. D., Corpas, F. J., Rodr\u00edguez-Garc\u00eda, M. I., & Del R\u00edo, L. A. (1998). Identification and immunolocalization of superoxide dismutase isoenzymes of olive pollen. Physiologia Plantarum, 104(4), 772\u2013776.","journal-title":"Physiologia Plantarum"},{"issue":"9","key":"9300_CR4","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.1093\/pcp\/pch127","volume":"45","author":"JD Alch\u00e9","year":"2004","unstructured":"Alch\u00e9, J. D., M\u2019rani-Alaoui, M., Castro, A. J., & Rodriguez-Garcia, M. I. (2004). Ole e 1, the major allergen from olive (Olea europaea L.) pollen, increases its expression and is released to the culture medium during in vitro germination. Plant and Cell Physiology, 45(9), 1149\u20131157.","journal-title":"Plant and Cell Physiology"},{"key":"9300_CR5","first-page":"497","volume":"63","author":"M Arilla","year":"2008","unstructured":"Arilla, M., Ibarrola, I., Martinez, A., & Asturias, J. (2008). Quantification of Ole e 9 content in O. europaea pollen extracts. Allergy, 63, 497.","journal-title":"Allergy"},{"issue":"3","key":"9300_CR6","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/S0091-6749(97)70250-1","volume":"100","author":"JA Asturias","year":"1997","unstructured":"Asturias, J. A., Arilla, M. C., GomezBayon, N., Martinez, J., Martinez, A., & Palacios, R. (1997). Cloning and expression of the panallergen profilin and the major allergen (Ole e 1) from olive tree pollen. Journal of Allergy and Clinical Immunology, 100(3), 365\u2013372.","journal-title":"Journal of Allergy and Clinical Immunology"},{"issue":"2","key":"9300_CR7","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.pep.2005.04.012","volume":"44","author":"P Barral","year":"2005","unstructured":"Barral, P., Batanero, E., Villalba, M., & Rodriguez, R. (2005). Expression of the major olive pollen allergen Ole e 10 in the yeast Pichia pastoris: Evidence of post-translational modifications. Protein Expression and Purification, 44(2), 147\u2013154.","journal-title":"Protein Expression and Purification"},{"issue":"2","key":"9300_CR8","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1016\/j.pep.2004.06.012","volume":"37","author":"P Barral","year":"2004","unstructured":"Barral, P., Tejera, M. L., Trevino, M. A., Batanero, E., Villalba, M., Bruix, M., et al. (2004). Recombinant expression of Ole e 6, a Cys-enriched pollen allergen, in Pichia pastoris yeast: Detection of partial oxidation of methionine by NMR. Protein Expression and Purification, 37(2), 336\u2013343.","journal-title":"Protein Expression and Purification"},{"issue":"3","key":"9300_CR9","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1111\/j.1432-1033.1996.00772.x","volume":"241","author":"E Batanero","year":"1996","unstructured":"Batanero, E., Villalba, M., Ledesma, A., Puente, X. S., & Rodriguez, R. (1996). Ole e 3, an olive-tree allergen, belongs to a widespread family of pollen proteins. European Journal of Biochemistry, 241(3), 772\u2013778.","journal-title":"European Journal of Biochemistry"},{"issue":"2","key":"9300_CR10","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/S0091-6749(98)70385-9","volume":"101","author":"L Boluda","year":"1998","unstructured":"Boluda, L., Alonso, C., & Fernandez-Caldas, E. (1998). Purification, characterization, and partial sequencing of two new allergens of Olea europaea. Journal of Allergy and Clinical Immunology, 101(2), 210\u2013216.","journal-title":"Journal of Allergy and Clinical Immunology"},{"issue":"1\u20132","key":"9300_CR11","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","volume":"72","author":"MM Bradford","year":"1976","unstructured":"Bradford, M. M. (1976). Rapid and sensitive method for quantitation of microgram quantities of protein utilizing principle of protein-dye binding. Analytical Biochemistry, 72(1\u20132), 248\u2013254.","journal-title":"Analytical Biochemistry"},{"issue":"4","key":"9300_CR12","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1034\/j.1398-9995.2002.03384.x","volume":"57","author":"J Carn\u00e9s S\u00e1nchez","year":"2002","unstructured":"Carn\u00e9s S\u00e1nchez, J., Iraola, V. M., Sastre, J., Florido, F., Boluda, L., & Fern\u00e1ndez-Caldas, E. (2002). Allergenicity and immunochemical characterization of six varieties of Olea europaea. Allergy, 57(4), 313\u2013318.","journal-title":"Allergy"},{"issue":"4","key":"9300_CR13","first-page":"159","volume":"25","author":"M Casanovas","year":"1997","unstructured":"Casanovas, M., Florido, F., de San Pedro, B. S., Gonzalez, P., Martinez-Alzamora, F., Maranon, F., et al. (1997). Sensitization to Olea europaea: Geographical differences and discrepancies. Allergologia Immunopathology (Madrid), 25(4), 159\u2013166.","journal-title":"Allergologia Immunopathology (Madrid)"},{"issue":"5","key":"9300_CR14","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1002\/pmic.200900192","volume":"10","author":"AJ Castro","year":"2010","unstructured":"Castro, A. J., Bednarczyk, A., Schaeffer-Reiss, C., Rodriguez-Garcia, M. I., Van Dorsselaer, A., & Alch\u00e9, J. D. (2010). Screening of Ole e 1 polymorphism among olive cultivars by peptide mapping and N-glycopeptide analysis. Proteomics, 10(5), 953\u2013962.","journal-title":"Proteomics"},{"key":"9300_CR15","unstructured":"Castro, L., Rodriguez, R., & Villalba, M. (2008). Ole e 12, an allergen from olive pollen, is an isoflavone reductase. EMBL\/GenBank\/DDBJ Databases 2008. http:\/\/www.uniprot.org\/uniprot\/E1U332 ."},{"issue":"2","key":"9300_CR16","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1159\/000092532","volume":"140","author":"O Duffort","year":"2006","unstructured":"Duffort, O., Palomares, O., Lombardero, M., Villalba, M., Barber, D., Rodriguez, R., et al. (2006). Variability of Ole e 9 allergen in olive pollen extracts: Relevance of minor allergens in immunotherapy treatments. International Archives of Allergy and Immunology, 140(2), 131\u2013138.","journal-title":"International Archives of Allergy and Immunology"},{"key":"9300_CR17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.talanta.2012.01.016","volume":"92","author":"C Esteve","year":"2012","unstructured":"Esteve, C., Montealegre, C., Marina, M. L., & Garcia, M. C. (2012). Analysis of olive allergens. Talanta, 92, 1\u201314.","journal-title":"Talanta"},{"issue":"5","key":"9300_CR18","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1016\/S1081-1206(10)60761-2","volume":"98","author":"E Fernandez-Caldas","year":"2007","unstructured":"Fernandez-Caldas, E., Carnes, J., Iraola, V., & Casanovas, M. (2007). Comparison of the allergenicity and Ole e 1 content of 6 varieties of Olea europaea pollen collected during 5 consecutive years. Annals of Allergy, Asthma & Immunology, 98(5), 464\u2013470.","journal-title":"Annals of Allergy, Asthma & Immunology"},{"issue":"5","key":"9300_CR19","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1111\/j.1398-9995.1996.tb04624.x","volume":"51","author":"C GellerBernstein","year":"1996","unstructured":"GellerBernstein, C., Arad, G., Keynan, N., Lahoz, C., Cardaba, B., & Waisel, Y. (1996). Hypersensitivity to pollen of Olea europaea in Israel. Allergy, 51(5), 356\u2013359.","journal-title":"Allergy"},{"key":"9300_CR20","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/1471-2229-8-10","volume":"8","author":"A Hamman-Khalifa","year":"2008","unstructured":"Hamman-Khalifa, A., Castro, A. J., Jimenez-Lopez, J. C., Rodriguez-Garcia, M. I., & Alch\u00e9, J. D. (2008). Olive cultivar origin is a major cause of polymorphism for Ole e 1 pollen allergen. BMC Plant Biology, 8, 10.","journal-title":"BMC Plant Biology"},{"issue":"30","key":"9300_CR21","doi-asserted-by":"crossref","first-page":"27959","DOI":"10.1074\/jbc.M103041200","volume":"276","author":"S Huecas","year":"2001","unstructured":"Huecas, S., Villalba, M., & Rodriguez, R. (2001). Ole e 9, a major olive pollen allergen is a 1,3-beta-glucanase\u2014Isolation, characterization, amino acid sequence, and tissue specificity. Journal of Biological Chemistry, 276(30), 27959\u201327966.","journal-title":"Journal of Biological Chemistry"},{"issue":"3","key":"9300_CR22","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1080\/09540105.2011.617357","volume":"23","author":"ZW Jaradat","year":"2012","unstructured":"Jaradat, Z. W., Al Bzour, A., Ababneh, Q., Shdiefat, S., Jaradat, S., & Al Domi, H. (2012). Identification of allergenic pollen grains in 36 olive (Olea europaea) cultivars grown in Jordan. Food and Agricultural Immunology, 23(3), 255\u2013264.","journal-title":"Food and Agricultural Immunology"},{"issue":"2","key":"9300_CR23","doi-asserted-by":"crossref","first-page":"e30878","DOI":"10.1371\/journal.pone.0030878","volume":"7","author":"JC Jimenez-Lopez","year":"2012","unstructured":"Jimenez-Lopez, J. C., Morales, S., Castro, A. J., Volkmann, D., Rodriguez-Garcia, M. I., & Alch\u00e9, J. D. (2012). Characterization of profilin polymorphism in pollen with a focus on multifunctionality. PLoS One, 7(2), e30878.","journal-title":"PLoS One"},{"issue":"5259","key":"9300_CR24","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","volume":"227","author":"UK Laemmli","year":"1970","unstructured":"Laemmli, U. K. (1970). Cleavage of structural proteins during assembly of head of bacteriophage-T4. Nature, 227(5259), 680\u2013685.","journal-title":"Nature"},{"issue":"4","key":"9300_CR25","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/0161-5890(88)90028-4","volume":"25","author":"P Lauzurica","year":"1988","unstructured":"Lauzurica, P., Maruri, N., Galocha, B., Gonzalez, J., Diaz, R., Palomino, P., et al. (1988). Olive (Olea europea) pollen allergens-II. Isolation and characterization of two major antigens. Molecular Immunology, 25(4), 337\u2013344.","journal-title":"Molecular Immunology"},{"issue":"5","key":"9300_CR26","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1111\/j.1398-9995.1998.tb04090.x","volume":"53","author":"A Ledesma","year":"1998","unstructured":"Ledesma, A., Rodriguez, R., & Villalba, M. (1998a). Olive-pollen profilin. Molecular and immunologic properties. Allergy, 53(5), 520\u2013526.","journal-title":"Allergy"},{"issue":"2","key":"9300_CR27","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1046\/j.1432-1327.1998.2580454.x","volume":"258","author":"A Ledesma","year":"1998","unstructured":"Ledesma, A., Villalba, M., Batanero, E., & Rodriguez, R. (1998b). Molecular cloning and expression of active Ole e 3, a major allergen from olive-tree pollen and member of a novel family of Ca2+-binding proteins (polcalcins) involved in allergy. European Journal of Biochemistry, 258(2), 454\u2013459.","journal-title":"European Journal of Biochemistry"},{"issue":"1","key":"9300_CR28","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/S0014-5793(99)01790-1","volume":"466","author":"A Ledesma","year":"2000","unstructured":"Ledesma, A., Villalba, M., & Rodriguez, R. (2000). Cloning, expression and characterization of a novel four EF-hand Ca2+-binding protein from olive pollen with allergenic activity. FEBS Letters, 466(1), 192\u2013196.","journal-title":"FEBS Letters"},{"key":"9300_CR29","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1034\/j.1398-9995.2002.057s71017.x","volume":"57","author":"A Mart\u00ednez","year":"2002","unstructured":"Mart\u00ednez, A., Asturias, J. A., Monteseir\u00edn, J., Moreno, V., Garc\u00eda-Cubillana, A., Hern\u00e1ndez, M., et al. (2002). The allergenic relevance of profilin (Ole e 2) from Olea europaea pollen. Allergy, 57, 17\u201323.","journal-title":"Allergy"},{"issue":"9\u201310","key":"9300_CR30","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1002\/elps.201100667","volume":"33","author":"S Morales","year":"2012","unstructured":"Morales, S., Castro, A. J., Jimenez-Lopez, J. C., Florido, F., Rodriguez-Garcia, M. I., & Alch\u00e9, J. D. (2012). A novel multiplex method for the simultaneous detection and relative quantitation of pollen allergens. Electrophoresis, 33(9\u201310), 1367\u20131374.","journal-title":"Electrophoresis"},{"issue":"2","key":"9300_CR31","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1111\/j.1365-2818.2008.02044.x","volume":"231","author":"S Morales","year":"2008","unstructured":"Morales, S., Jimenez-Lopez, J. C., Castro, A. J., Rodriguez-Garcia, M. I., & Alch\u00e9, J. D. (2008). Olive pollen profilin (Ole e 2 allergen) co-localizes with highly active areas of the actin cytoskeleton and is released to the culture medium during in vitro pollen germination. Journal of Microscopy, 231(2), 332\u2013341.","journal-title":"Journal of Microscopy"},{"issue":"1","key":"9300_CR32","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/S1081-1206(10)61371-3","volume":"97","author":"O Palomares","year":"2006","unstructured":"Palomares, O., Villalba, M., Quiralte, J., & Rodriguez, R. (2006). Allergenic contribution of the IgE-reactive domains of the 1,3-beta-glucanase Ole e 9: Diagnostic value in olive pollen allergy. Annals of Allergy, Asthma & Immunology, 97(1), 61\u201365.","journal-title":"Annals of Allergy, Asthma & Immunology"},{"issue":"Pt 3","key":"9300_CR33","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1042\/bj20020423","volume":"369","author":"O Palomares","year":"2003","unstructured":"Palomares, O., Villalba, M., & Rodriguez, R. (2003). The C-terminal segment of the 1,3-beta-glucanase Ole e 9 from olive (Olea europaea) pollen is an independent domain with allergenic activity: Expression in Pichia pastoris and characterization. Biochemical Journal, 369(Pt 3), 593\u2013601.","journal-title":"Biochemical Journal"},{"key":"9300_CR34","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1034\/j.1398-9995.2002.057s71047.x","volume":"57","author":"J Quiralte","year":"2002","unstructured":"Quiralte, J., Florido, F., Arias de Saavedra, J. M., G\u00f3mez, A., de San, S\u00e1enz., Pedro, B., et al. (2002). Olive allergen-specific IgE responses in patients with Olea europaea pollinosis. Allergy, 57, 47\u201352.","journal-title":"Allergy"},{"issue":"Suppl 71","key":"9300_CR35","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1034\/j.1398-9995.2002.057s71006.x","volume":"57","author":"R Rodriguez","year":"2002","unstructured":"Rodriguez, R., Villalba, M., Batanero, E., Gonzalez, E. M., Monsalve, R. I., Huecas, S., et al. (2002). Allergenic diversity of the olive pollen. Allergy, 57(Suppl 71), 6\u201316.","journal-title":"Allergy"},{"key":"9300_CR36","first-page":"500","volume":"63","author":"G Salamanca","year":"2008","unstructured":"Salamanca, G., Moreno, C., Quiralte, J., Barber, D., Villalba, M., & Rodriguez, R. (2008). Immunological characterisation of Ole e 11, a major olive pollen allergen. Allergy, 63, 500.","journal-title":"Allergy"},{"issue":"13","key":"9300_CR37","doi-asserted-by":"crossref","first-page":"2729","DOI":"10.1111\/j.1742-4658.2010.07689.x","volume":"277","author":"G Salamanca","year":"2010","unstructured":"Salamanca, G., Rodriguez, R., Quiralte, J., Moreno, C., Pascual, C. Y., Barber, D., et al. (2010). Pectin methylesterases of pollen tissue, a major allergen in olive tree. FEBS Journal, 277(13), 2729\u20132739.","journal-title":"FEBS Journal"},{"issue":"3","key":"9300_CR38","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/S0960-9822(00)00050-6","volume":"4","author":"CJ Staiger","year":"1994","unstructured":"Staiger, C. J., Yuan, M., Valenta, R., Shaw, P. J., Warn, R. M., & Lloyd, C. W. (1994). Microinjected profilin affects cytoplasmic streaming in plant-cells by rapidly depolymerizing actin microfilaments. Current Biology, 4(3), 215\u2013219.","journal-title":"Current Biology"},{"issue":"4","key":"9300_CR39","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1016\/S0091-6749(99)70290-3","volume":"104","author":"ML Tejera","year":"1999","unstructured":"Tejera, M. L., Villalba, M., Batanero, E., & Rodriguez, R. (1999). Identification, isolation, and characterization of Ole e 7, a new allergen of olive tree pollen. Journal of Allergy and Clinical Immunology, 104(4), 797\u2013802.","journal-title":"Journal of Allergy and Clinical Immunology"},{"issue":"7","key":"9300_CR40","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1016\/0161-5890(90)90005-K","volume":"27","author":"AW Wheeler","year":"1990","unstructured":"Wheeler, A. W., Hickman, B. E., & Fox, B. (1990). Heterogeneity of a major allergen from Olive (Olea europea) pollen. Molecular Immunology, 27(7), 631\u2013636.","journal-title":"Molecular Immunology"},{"key":"9300_CR41","first-page":"41","volume-title":"Current insights in pollen allergens","author":"K Zienkiewicz","year":"2012","unstructured":"Zienkiewicz, K., Garc\u00eda-Quir\u00f3s, E., Alch\u00e9, J. D., Rodr\u00edguez-Garc\u00eda, M. I., & Castro, A. J. (2012). Simultaneous detection and quantitation of pollen allergens using a fluorescence-based 2-D multiplex approach. In J. C. Jim\u00e9nez-L\u00f3pez (Ed.), Current insights in pollen allergens (pp. 41\u201356). Croatia: Intech Open Science."}],"container-title":["Aerobiologia"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10453-013-9300-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s10453-013-9300-8\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10453-013-9300-8","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,15]],"date-time":"2022-02-15T21:07:13Z","timestamp":1644959233000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s10453-013-9300-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,5,3]]},"references-count":41,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2013,12]]}},"alternative-id":["9300"],"URL":"https:\/\/doi.org\/10.1007\/s10453-013-9300-8","relation":{},"ISSN":["0393-5965","1573-3025"],"issn-type":[{"value":"0393-5965","type":"print"},{"value":"1573-3025","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,5,3]]}}}