{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,1,28]],"date-time":"2024-01-28T00:08:05Z","timestamp":1706400485638},"reference-count":42,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2004,6,1]]},"abstract":"<jats:p>Dendritic cells (DCs) are antigen-presenting cells (APCs) capable of capturing haptens and to process and present them to T lymphocytes. In order to sensitize T cells for contact hypersensitivity (CHS), skin DCs suffer a maturation process with modifications on their surface molecules. The aim of this work was to evaluate changes induced by two contact sensitizers, 2,4-dinitrofluorobenzene (DNFB) and nickel sulfate (NiSO4), and a non-sensitizer 2,4-dichloronitrobenzene (DCNB), on the protein levels of two activation markers, CD40 and IL-12 receptor (IL-12R), in a mouse skin dendritic cell line (FSDC). The expression of CD40 and IL-12R proteins was evaluated by western blot assay and direct immunofluorescence microscopy. The results showed that CD40 and IL-12R expression increased significantly after cell exposure to NiSO4 and DNFB, although DNFB exhibited a stronger activity. There was no effect with DCNB. The epidermal cytokine granulocyte\u2013macrophage colony-stimulating factor (GM-CSF), also used in the experiments, slightly increased the expression of both CD40 and IL-12R and when tested together with the sensitizers the effect was partially additive. The results suggest that the sensitizers DNFB and NiSO4 are directly involved on the changes of the surface markers CD40 and IL-12R in skin DCs, during the sensitization phase of CHS, and this effect may be enhanced by GM-CSF. In contrast, no effect was observed with DCNB.<\/jats:p>","DOI":"10.1007\/s10540-005-2580-7","type":"journal-article","created":{"date-parts":[[2005,7,18]],"date-time":"2005-07-18T19:44:57Z","timestamp":1121715897000},"page":"191-202","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["The Sensitizers Nickel Sulfate and 2,4-dinitrofluorobenzene Increase CD40 and IL-12 Receptor Expression in a Fetal Skin Dendritic Cell Line"],"prefix":"10.1042","volume":"24","author":[{"given":"Ana Lu\u00edsa","family":"Vital","sequence":"first","affiliation":[{"name":"Faculdade de Farm\u00e1cia, Universidade de Coimbra, Rua do Norte, 3000-295, Coimbra, Portugal"},{"name":"Centro de Neuroci\u00eancias e Biologia Celular, Universidade de Coimbra, 3004-517, Coimbra, Portugal"}]},{"given":"Margarida","family":"Gon\u00e7alo","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina (Servi\u00e7o de Dermatologia), Hospital da Universidade de Coimbra, 3000-075, Coimbra, Portugal, +351-239-480217"}]},{"given":"Maria Teresa","family":"Cruz","sequence":"additional","affiliation":[{"name":"Faculdade de Farm\u00e1cia, Universidade de Coimbra, Rua do Norte, 3000-295, Coimbra, Portugal"},{"name":"Centro de Neuroci\u00eancias e Biologia Celular, Universidade de Coimbra, 3004-517, Coimbra, Portugal"}]},{"given":"Am\u00e9rico","family":"Figueiredo","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina (Servi\u00e7o de Dermatologia), Hospital da Universidade de Coimbra, 3000-075, Coimbra, Portugal, +351-239-480217"}]},{"given":"Carlos B.","family":"Duarte","sequence":"additional","affiliation":[{"name":"Centro de Neuroci\u00eancias e Biologia Celular, Universidade de Coimbra, 3004-517, Coimbra, Portugal"}]},{"given":"Maria Celeste","family":"Lopes","sequence":"additional","affiliation":[{"name":"Faculdade de Farm\u00e1cia, Universidade de Coimbra, Rua do Norte, 3000-295, Coimbra, Portugal"},{"name":"Centro de Neuroci\u00eancias e Biologia Celular, Universidade de Coimbra, 3004-517, Coimbra, Portugal"}]}],"member":"288","published-online":{"date-parts":[[2005,7,18]]},"reference":[{"key":"2580_CR1","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1046\/j.1365-2567.2000.00087.x","volume":"101","author":"S. 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