{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T08:25:17Z","timestamp":1767860717607,"version":"3.49.0"},"reference-count":51,"publisher":"Springer Science and Business Media LLC","issue":"7","license":[{"start":{"date-parts":[[2021,5,25]],"date-time":"2021-05-25T00:00:00Z","timestamp":1621900800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2021,5,25]],"date-time":"2021-05-25T00:00:00Z","timestamp":1621900800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/501100011929","name":"Programa Operacional Tem\u00e1tico Factores de Competitividade","doi-asserted-by":"publisher","award":["Project POCI-01-0145-FEDER007491"],"award-info":[{"award-number":["Project POCI-01-0145-FEDER007491"]}],"id":[{"id":"10.13039\/501100011929","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["Project UID\/Multi\/00709\/2019"],"award-info":[{"award-number":["Project UID\/Multi\/00709\/2019"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Programa Operacional do Centro, Centro 2020\u201d through the funding of the ICON project (Interdisciplinary Challenges On Neurodegeneration","award":["CENTRO-01-0145-FEDER-000013"],"award-info":[{"award-number":["CENTRO-01-0145-FEDER-000013"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Cell Mol Neurobiol"],"published-print":{"date-parts":[[2022,10]]},"DOI":"10.1007\/s10571-021-01103-5","type":"journal-article","created":{"date-parts":[[2021,5,25]],"date-time":"2021-05-25T08:02:52Z","timestamp":1621929772000},"page":"2289-2304","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["In Vitro Model for Ischemic Stroke: Functional Analysis of Vascular Smooth Muscle Cells"],"prefix":"10.1007","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1875-0144","authenticated-orcid":false,"given":"Melissa","family":"Mariana","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2890-4891","authenticated-orcid":false,"given":"Claudio","family":"Roque","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7942-0432","authenticated-orcid":false,"given":"Gra\u00e7a","family":"Baltazar","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4823-5701","authenticated-orcid":false,"given":"Elisa","family":"Cairrao","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2021,5,25]]},"reference":[{"issue":"2","key":"1103_CR1","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1046\/j.1471-4159.2002.00827.x","volume":"81","author":"A Almeida","year":"2002","unstructured":"Almeida A, Delgado-Esteban M, Bolanos JP, Medina JM (2002) Oxygen and glucose deprivation induces mitochondrial dysfunction and oxidative stress in neurones but not in astrocytes in primary culture. J Neurochem 81(2):207\u2013217. https:\/\/doi.org\/10.1046\/j.1471-4159.2002.00827.x","journal-title":"J Neurochem"},{"issue":"1","key":"1103_CR2","doi-asserted-by":"publisher","first-page":"318","DOI":"10.1152\/japplphysiol.00937.2005","volume":"100","author":"J Andresen","year":"1985","unstructured":"Andresen J, Shafi NI, Bryan RM Jr (1985) (2006) Endothelial influences on cerebrovascular tone. J Appl Physiol 100(1):318\u2013327. https:\/\/doi.org\/10.1152\/japplphysiol.00937.2005","journal-title":"J Appl Physiol"},{"issue":"8","key":"1103_CR3","doi-asserted-by":"publisher","first-page":"1815","DOI":"10.1161\/STROKEAHA.109.577635","volume":"41","author":"DN Atochin","year":"2010","unstructured":"Atochin DN, Yuzawa I, Li Q, Rauwerdink KM, Malhotra R, Chang J, Brouckaert P, Ayata C, Moskowitz MA, Bloch KD, Huang PL, Buys ES (2010) Soluble guanylate cyclase alpha1beta1 limits stroke size and attenuates neurological injury. Stroke 41(8):1815\u20131819. https:\/\/doi.org\/10.1161\/STROKEAHA.109.577635","journal-title":"Stroke"},{"key":"1103_CR4","doi-asserted-by":"publisher","unstructured":"Bacakova L, Travnickova M, Filova E, Mat\u011bjka R, Stepanovska J, Musilkova J, Zarubova J, Molitor M (2018) The role of vascular smooth muscle cells in the physiology and pathophysiology of blood vessels. In: Sakuma K (ed) Muscle cell and tissue\u2014current status of research field. IntechOpen, pp 229\u2013257. https:\/\/doi.org\/10.5772\/intechopen.77115","DOI":"10.5772\/intechopen.77115"},{"key":"1103_CR5","doi-asserted-by":"publisher","first-page":"88","DOI":"10.3389\/fnmol.2017.00088","volume":"10","author":"A Becerra-Calixto","year":"2017","unstructured":"Becerra-Calixto A, Cardona-Gomez GP (2017) The role of astrocytes in neuroprotection after brain stroke: potential in cell therapy. Front Mol Neurosci 10:88. https:\/\/doi.org\/10.3389\/fnmol.2017.00088","journal-title":"Front Mol Neurosci"},{"issue":"3\u20134","key":"1103_CR6","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1007\/s11626-008-9161-6","volume":"45","author":"E Cairrao","year":"2009","unstructured":"Cairrao E, Santos-Silva AJ, Alvarez E, Correia I, Verde I (2009) Isolation and culture of human umbilical artery smooth muscle cells expressing functional calcium channels. Vitro Cell Dev Biol Anim 45(3\u20134):175\u2013184. https:\/\/doi.org\/10.1007\/s11626-008-9161-6","journal-title":"Vitro Cell Dev Biol Anim"},{"key":"1103_CR7","doi-asserted-by":"publisher","first-page":"393","DOI":"10.1007\/978-1-4939-7571-6_31","volume":"1727","author":"R Crupi","year":"2018","unstructured":"Crupi R, Di Paola R, Esposito E, Cuzzocrea S (2018) Middle cerebral artery occlusion by an intraluminal suture method. Methods Mol Biol 1727:393\u2013401. https:\/\/doi.org\/10.1007\/978-1-4939-7571-6_31","journal-title":"Methods Mol Biol"},{"issue":"16","key":"1103_CR8","doi-asserted-by":"publisher","first-page":"4912","DOI":"10.1046\/j.1432-1327.2000.01597.x","volume":"267","author":"R Dringen","year":"2000","unstructured":"Dringen R, Gutterer JM, Hirrlinger J (2000) Glutathione metabolism in brain metabolic interaction between astrocytes and neurons in the defense against reactive oxygen species. Eur J Biochem 267(16):4912\u20134916. https:\/\/doi.org\/10.1046\/j.1432-1327.2000.01597.x","journal-title":"Eur J Biochem"},{"issue":"3","key":"1103_CR9","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1159\/000441085","volume":"45","author":"VL Feigin","year":"2015","unstructured":"Feigin VL, Krishnamurthi RV, Parmar P, Norrving B, Mensah GA, Bennett DA, Barker-Collo S, Moran AE, Sacco RL, Truelsen T, Davis S, Pandian JD, Naghavi M, Forouzanfar MH, Nguyen G, Johnson CO, Vos T, Meretoja A, Murray CJ, Roth GA (2015) Update on the global burden of ischemic and hemorrhagic stroke in 1990\u20132013: the GBD 2013 Study. Neuroepidemiology 45(3):161\u2013176. https:\/\/doi.org\/10.1159\/000441085","journal-title":"Neuroepidemiology"},{"key":"1103_CR10","doi-asserted-by":"publisher","first-page":"96","DOI":"10.1016\/j.neuroscience.2015.03.064","volume":"323","author":"JA Filosa","year":"2016","unstructured":"Filosa JA, Morrison HW, Iddings JA, Du W, Kim KJ (2016) Beyond neurovascular coupling, role of astrocytes in the regulation of vascular tone. Neuroscience 323:96\u2013109. https:\/\/doi.org\/10.1016\/j.neuroscience.2015.03.064","journal-title":"Neuroscience"},{"key":"1103_CR11","doi-asserted-by":"publisher","DOI":"10.3791\/61388","author":"G Gava-Junior","year":"2020","unstructured":"Gava-Junior G, Roque C, Mendes-Oliveira J, Bernardino AC, Serrenho I, Pires JP, Baltazar G (2020) A cell culture model for studying the role of neuron-glia interactions in ischemia. J vis Exp. https:\/\/doi.org\/10.3791\/61388","journal-title":"J vis Exp"},{"issue":"4","key":"1103_CR12","doi-asserted-by":"publisher","first-page":"630","DOI":"10.1111\/j.1750-3639.2008.00226.x","volume":"19","author":"G Gurer","year":"2009","unstructured":"Gurer G, Gursoy-Ozdemir Y, Erdemli E, Can A, Dalkara T (2009) Astrocytes are more resistant to focal cerebral ischemia than neurons and die by a delayed necrosis. Brain Pathol 19(4):630\u2013641. https:\/\/doi.org\/10.1111\/j.1750-3639.2008.00226.x","journal-title":"Brain Pathol"},{"issue":"6","key":"1103_CR13","doi-asserted-by":"publisher","first-page":"908","DOI":"10.1165\/rcmb.2003-0287OC2003-0287OC","volume":"30","author":"PM Hassoun","year":"2004","unstructured":"Hassoun PM, Filippov G, Fogel M, Donaldson C, Kayyali US, Shimoda LA, Bloch KD (2004) Hypoxia decreases expression of soluble guanylate cyclase in cultured rat pulmonary artery smooth muscle cells. Am J Respir Cell Mol Biol 30(6):908\u2013913. https:\/\/doi.org\/10.1165\/rcmb.2003-0287OC2003-0287OC","journal-title":"Am J Respir Cell Mol Biol"},{"issue":"7613","key":"1103_CR14","doi-asserted-by":"publisher","first-page":"551","DOI":"10.1038\/nature18928","volume":"535","author":"K Hayakawa","year":"2016","unstructured":"Hayakawa K, Esposito E, Wang X, Terasaki Y, Liu Y, Xing C, Ji X, Lo EH (2016) Transfer of mitochondria from astrocytes to neurons after stroke. Nature 535(7613):551\u2013555. https:\/\/doi.org\/10.1038\/nature18928","journal-title":"Nature"},{"issue":"7\u20138","key":"1103_CR15","doi-asserted-by":"publisher","first-page":"1057","DOI":"10.1007\/s10571-006-9008-1","volume":"26","author":"KA Hossmann","year":"2006","unstructured":"Hossmann KA (2006) Pathophysiology and therapy of experimental stroke. Cell Mol Neurobiol 26(7\u20138):1057\u20131083. https:\/\/doi.org\/10.1007\/s10571-006-9008-1","journal-title":"Cell Mol Neurobiol"},{"key":"1103_CR16","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20020282","author":"L Huang","year":"2019","unstructured":"Huang L, Nakamura Y, Lo EH, Hayakawa K (2019) Astrocyte signaling in the neurovascular unit after central nervous system injury. Int J Mol Sci. https:\/\/doi.org\/10.3390\/ijms20020282","journal-title":"Int J Mol Sci"},{"issue":"1","key":"1103_CR17","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1016\/j.neuron.2017.07.030","volume":"96","author":"C Iadecola","year":"2017","unstructured":"Iadecola C (2017) The neurovascular unit coming of age: a journey through neurovascular coupling in health and disease. Neuron 96(1):17\u201342. https:\/\/doi.org\/10.1016\/j.neuron.2017.07.030","journal-title":"Neuron"},{"issue":"9","key":"1103_CR18","doi-asserted-by":"publisher","first-page":"634","DOI":"10.2471\/BLT.16.181636BLT.16.181636","volume":"94","author":"W Johnson","year":"2016","unstructured":"Johnson W, Onuma O, Owolabi M, Sachdev S (2016) Stroke: a global response is needed. Bull World Health Organ 94(9):634-634A. https:\/\/doi.org\/10.2471\/BLT.16.181636BLT.16.181636","journal-title":"Bull World Health Organ"},{"issue":"3","key":"1103_CR19","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1161\/01.hyp.36.3.383","volume":"36","author":"J Jordan","year":"2000","unstructured":"Jordan J, Shannon JR, Diedrich A, Black B, Costa F, Robertson D, Biaggioni I (2000) Interaction of carbon dioxide and sympathetic nervous system activity in the regulation of cerebral perfusion in humans. Hypertension 36(3):383\u2013388. https:\/\/doi.org\/10.1161\/01.hyp.36.3.383","journal-title":"Hypertension"},{"issue":"4","key":"1103_CR20","doi-asserted-by":"publisher","first-page":"338","DOI":"10.1161\/01.res.85.4.338","volume":"85","author":"S Li","year":"1999","unstructured":"Li S, Sims S, Jiao Y, Chow LH, Pickering JG (1999) Evidence from a novel human cell clone that adult vascular smooth muscle cells can convert reversibly between noncontractile and contractile phenotypes. Circ Res 85(4):338\u2013348. https:\/\/doi.org\/10.1161\/01.res.85.4.338","journal-title":"Circ Res"},{"issue":"5","key":"1103_CR21","doi-asserted-by":"publisher","first-page":"655","DOI":"10.2174\/1570161111311050008","volume":"11","author":"LD Longo","year":"2013","unstructured":"Longo LD, Goyal R (2013) Cerebral artery signal transduction mechanisms: developmental changes in dynamics and Ca2+ sensitivity. Curr Vasc Pharmacol 11(5):655\u2013711. https:\/\/doi.org\/10.2174\/1570161111311050008","journal-title":"Curr Vasc Pharmacol"},{"issue":"6","key":"1103_CR22","doi-asserted-by":"publisher","first-page":"723","DOI":"10.1177\/1933719115612926","volume":"23","author":"M Mariana","year":"2016","unstructured":"Mariana M, Feiteiro J, Cairrao E, Verde I (2016) Mifepristone is a vasodilator due to the inhibition of smooth muscle cells L-Type Ca2+ channels. Reprod Sci 23(6):723\u2013730. https:\/\/doi.org\/10.1177\/1933719115612926","journal-title":"Reprod Sci"},{"issue":"3","key":"1103_CR23","doi-asserted-by":"publisher","first-page":"762","DOI":"10.1074\/jbc.R116.760215","volume":"292","author":"HL McConnell","year":"2017","unstructured":"McConnell HL, Kersch CN, Woltjer RL, Neuwelt EA (2017) The translational significance of the neurovascular unit. J Biol Chem 292(3):762\u2013770. https:\/\/doi.org\/10.1074\/jbc.R116.760215","journal-title":"J Biol Chem"},{"issue":"12","key":"1103_CR24","doi-asserted-by":"publisher","first-page":"1619","DOI":"10.1038\/nn.4428","volume":"19","author":"A Mishra","year":"2016","unstructured":"Mishra A, Reynolds JP, Chen Y, Gourine AV, Rusakov DA, Attwell D (2016) Astrocytes mediate neurovascular signaling to capillary pericytes but not to arterioles. Nat Neurosci 19(12):1619\u20131627. https:\/\/doi.org\/10.1038\/nn.4428","journal-title":"Nat Neurosci"},{"key":"1103_CR25","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1016\/j.brainres.2010.05.091","volume":"1346","author":"L Monge","year":"2010","unstructured":"Monge L, Fernandez N, Salcedo A, Garcia-Villalon AL, Dieguez G (2010) Role of alpha-adrenoceptors and prostacyclin in the enhanced adrenergic reactivity of goat cerebral arteries after ischemia-reperfusion. Brain Res 1346:121\u2013131. https:\/\/doi.org\/10.1016\/j.brainres.2010.05.091","journal-title":"Brain Res"},{"issue":"4","key":"1103_CR26","doi-asserted-by":"publisher","first-page":"790","DOI":"10.1111\/apha.12250","volume":"210","author":"V Muoio","year":"2014","unstructured":"Muoio V, Persson PB, Sendeski MM (2014) The neurovascular unit\u2014concept review. Acta Physiol (oxf) 210(4):790\u2013798. https:\/\/doi.org\/10.1111\/apha.12250","journal-title":"Acta Physiol (oxf)"},{"issue":"3","key":"1103_CR27","doi-asserted-by":"publisher","first-page":"487","DOI":"10.1152\/physrev.1995.75.3.487","volume":"75","author":"GK Owens","year":"1995","unstructured":"Owens GK (1995) Regulation of differentiation of vascular smooth muscle cells. Physiol Rev 75(3):487\u2013517. https:\/\/doi.org\/10.1152\/physrev.1995.75.3.487","journal-title":"Physiol Rev"},{"key":"1103_CR28","doi-asserted-by":"publisher","first-page":"246","DOI":"10.1016\/j.neuint.2019.03.022","volume":"126","author":"T Ozaki","year":"2019","unstructured":"Ozaki T, Nakamura H, Kishima H (2019) Therapeutic strategy against ischemic stroke with the concept of neurovascular unit. Neurochem Int 126:246\u2013251. https:\/\/doi.org\/10.1016\/j.neuint.2019.03.022","journal-title":"Neurochem Int"},{"issue":"1","key":"1103_CR29","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1186\/s13041-016-0243-1","volume":"9","author":"Q Pan","year":"2016","unstructured":"Pan Q, He C, Liu H, Liao X, Dai B, Chen Y, Yang Y, Zhao B, Bihl J, Ma X (2016) Microvascular endothelial cells-derived microvesicles imply in ischemic stroke by modulating astrocyte and blood brain barrier function and cerebral blood flow. Mol Brain 9(1):63. https:\/\/doi.org\/10.1186\/s13041-016-0243-1","journal-title":"Mol Brain"},{"issue":"4\u20135","key":"1103_CR30","first-page":"295","volume":"18","author":"E Paternotte","year":"2008","unstructured":"Paternotte E, Gaucher C, Labrude P, Stoltz JF, Menu P (2008) Review: behaviour of endothelial cells faced with hypoxia. Biomed Mater Eng 18(4\u20135):295\u2013299","journal-title":"Biomed Mater Eng"},{"issue":"5","key":"1103_CR31","doi-asserted-by":"publisher","first-page":"782","DOI":"10.1016\/j.neuron.2011.08.009","volume":"71","author":"GC Petzold","year":"2011","unstructured":"Petzold GC, Murthy VN (2011) Role of astrocytes in neurovascular coupling. Neuron 71(5):782\u2013797. https:\/\/doi.org\/10.1016\/j.neuron.2011.08.009","journal-title":"Neuron"},{"issue":"3","key":"1103_CR32","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1007\/s12975-013-0306-x","volume":"5","author":"M Poittevin","year":"2014","unstructured":"Poittevin M, Lozeron P, Hilal R, Levy BI, Merkulova-Rainon T, Kubis N (2014) Smooth muscle cell phenotypic switching in stroke. Transl Stroke Res 5(3):377\u2013384. https:\/\/doi.org\/10.1007\/s12975-013-0306-x","journal-title":"Transl Stroke Res"},{"issue":"5","key":"1103_CR33","doi-asserted-by":"publisher","first-page":"502","DOI":"10.4103\/0253-7613.84950IJPharm-43-502","volume":"43","author":"S Purkayastha","year":"2011","unstructured":"Purkayastha S, Raven PB (2011) The functional role of the alpha-1 adrenergic receptors in cerebral blood flow regulation. Indian J Pharmacol 43(5):502\u2013506. https:\/\/doi.org\/10.4103\/0253-7613.84950IJPharm-43-502","journal-title":"Indian J Pharmacol"},{"key":"1103_CR34","doi-asserted-by":"publisher","first-page":"283","DOI":"10.2147\/NDT.S231944231944","volume":"16","author":"C Qin","year":"2020","unstructured":"Qin C, Yan X, Jin H, Zhang R, He Y, Sun X, Zhang Y, Guo ZN, Yang Y (2020) Effects of remote ischemic conditioning on cerebral hemodynamics in ischemic stroke. Neuropsychiatr Dis Treat 16:283\u2013299. https:\/\/doi.org\/10.2147\/NDT.S231944231944","journal-title":"Neuropsychiatr Dis Treat"},{"issue":"5","key":"1103_CR35","doi-asserted-by":"publisher","first-page":"502","DOI":"10.2174\/1567202616666191026122642","volume":"16","author":"P Quelhas","year":"2019","unstructured":"Quelhas P, Baltazar G, Cairrao E (2019) The neurovascular unit: focus on the regulation of arterial smooth muscle cells. Curr Neurovasc Res 16(5):502\u2013515. https:\/\/doi.org\/10.2174\/1567202616666191026122642","journal-title":"Curr Neurovasc Res"},{"key":"1103_CR36","doi-asserted-by":"publisher","DOI":"10.1016\/j.tice.2020.101400","author":"P Quelhas","year":"2020","unstructured":"Quelhas P, Baltazar G, Cairrao E (2020) Characterization of culture from smooth muscle cells isolated from rat middle cerebral arteries. Tissue Cell. https:\/\/doi.org\/10.1016\/j.tice.2020.101400","journal-title":"Tissue Cell"},{"issue":"3","key":"1103_CR37","doi-asserted-by":"publisher","first-page":"100","DOI":"10.1007\/bf03085963","volume":"15","author":"SS Rensen","year":"2007","unstructured":"Rensen SS, Doevendans PA, van Eys GJ (2007) Regulation and characteristics of vascular smooth muscle cell phenotypic diversity. Neth Heart J 15(3):100\u2013108. https:\/\/doi.org\/10.1007\/bf03085963","journal-title":"Neth Heart J"},{"issue":"8","key":"1103_CR38","doi-asserted-by":"publisher","first-page":"1521","DOI":"10.1007\/s10571-017-0483-310.1007\/s10571-017-0483-3","volume":"37","author":"C Roque","year":"2017","unstructured":"Roque C, Baltazar G (2017) Impact of astrocytes on the injury induced by in vitro ischemia. Cell Mol Neurobiol 37(8):1521\u20131528. https:\/\/doi.org\/10.1007\/s10571-017-0483-310.1007\/s10571-017-0483-3","journal-title":"Cell Mol Neurobiol"},{"key":"1103_CR39","doi-asserted-by":"publisher","first-page":"86","DOI":"10.1016\/j.pneurobio.2015.04.003","volume":"130","author":"D Rossi","year":"2015","unstructured":"Rossi D (2015) Astrocyte physiopathology: at the crossroads of intercellular networking, inflammation and cell death. Prog Neurobiol 130:86\u2013120. https:\/\/doi.org\/10.1016\/j.pneurobio.2015.04.003","journal-title":"Prog Neurobiol"},{"issue":"10","key":"1103_CR40","doi-asserted-by":"publisher","first-page":"1510","DOI":"10.2174\/13816128113199990465","volume":"20","author":"K Shah","year":"2014","unstructured":"Shah K, Abbruscato T (2014) The role of blood-brain barrier transporters in pathophysiology and pharmacotherapy of stroke. Curr Pharm Des 20(10):1510\u20131522. https:\/\/doi.org\/10.2174\/13816128113199990465","journal-title":"Curr Pharm Des"},{"issue":"11","key":"1103_CR41","doi-asserted-by":"publisher","first-page":"2055","DOI":"10.1038\/jcbfm.2012.107","volume":"32","author":"V Sharma","year":"2012","unstructured":"Sharma V, Ling TW, Rewell SS, Hare DL, Howells DW, Kourakis A, Wookey PJ (2012) A novel population of alpha-smooth muscle actin-positive cells activated in a rat model of stroke: an analysis of the spatio-temporal distribution in response to ischemia. J Cereb Blood Flow Metab 32(11):2055\u20132065. https:\/\/doi.org\/10.1038\/jcbfm.2012.107","journal-title":"J Cereb Blood Flow Metab"},{"issue":"2","key":"1103_CR42","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1161\/STROKEAHA.109.564229","volume":"41","author":"J Shimotake","year":"2010","unstructured":"Shimotake J, Derugin N, Wendland M, Vexler ZS, Ferriero DM (2010) Vascular endothelial growth factor receptor-2 inhibition promotes cell death and limits endothelial cell proliferation in a neonatal rodent model of stroke. Stroke 41(2):343\u2013349. https:\/\/doi.org\/10.1161\/STROKEAHA.109.564229","journal-title":"Stroke"},{"issue":"10","key":"1103_CR43","doi-asserted-by":"publisher","first-page":"1318","DOI":"10.1038\/s41593-018-0234-x10.1038\/s41593-018-0234-x","volume":"21","author":"MD Sweeney","year":"2018","unstructured":"Sweeney MD, Kisler K, Montagne A, Toga AW, Zlokovic BV (2018) The role of brain vasculature in neurodegenerative disorders. Nat Neurosci 21(10):1318\u20131331. https:\/\/doi.org\/10.1038\/s41593-018-0234-x10.1038\/s41593-018-0234-x","journal-title":"Nat Neurosci"},{"key":"1103_CR44","volume-title":"Acute Stroke","author":"P Tadi","year":"2021","unstructured":"Tadi P, Lui F (2020) Acute stroke (cerebrovascular accident)"},{"issue":"1","key":"1103_CR45","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1097\/00008506-199701000-00013","volume":"9","author":"H Tao","year":"1997","unstructured":"Tao H, Zhang LM, Castresana MR, Newman WH, Shillcutt SD (1997) Response of cultured cerebral artery smooth muscle cells to the nitric oxide vasodilators, nitroglycerin and sodium nitroprusside. J Neurosurg Anesthesiol 9(1):58\u201364. https:\/\/doi.org\/10.1097\/00008506-199701000-00013","journal-title":"J Neurosurg Anesthesiol"},{"issue":"3","key":"1103_CR46","doi-asserted-by":"publisher","first-page":"R742","DOI":"10.1152\/ajpregu.1998.275.3.R742","volume":"275","author":"GQ Teng","year":"1998","unstructured":"Teng GQ, Williams J, Zhang L, Purdy R, Pearce WJ (1998) Effects of maturation, artery size, and chronic hypoxia on 5-HT receptor type in ovine cranial arteries. Am J Physiol 275(3):R742-753. https:\/\/doi.org\/10.1152\/ajpregu.1998.275.3.R742","journal-title":"Am J Physiol"},{"key":"1103_CR47","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1186\/s13054-015-0761-810.1186\/s13054-015-0761-8","volume":"19","author":"TH Tsai","year":"2015","unstructured":"Tsai TH, Lu CH, Wallace CG, Chang WN, Chen SF, Huang CR, Tsai NW, Lan MY, Sung PH, Liu CF, Yip HK (2015) Erythropoietin improves long-term neurological outcome in acute ischemic stroke patients: a randomized, prospective, placebo-controlled clinical trial. Crit Care 19:49. https:\/\/doi.org\/10.1186\/s13054-015-0761-810.1186\/s13054-015-0761-8","journal-title":"Crit Care"},{"issue":"1","key":"1103_CR48","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1186\/1750-1326-6-11","volume":"6","author":"TM Woodruff","year":"2011","unstructured":"Woodruff TM, Thundyil J, Tang SC, Sobey CG, Taylor SM, Arumugam TV (2011) Pathophysiology, treatment, and animal and cellular models of human ischemic stroke. Mol Neurodegener 6(1):11. https:\/\/doi.org\/10.1186\/1750-1326-6-11","journal-title":"Mol Neurodegener"},{"key":"1103_CR49","doi-asserted-by":"publisher","first-page":"294","DOI":"10.3389\/fimmu.2020.00294","volume":"11","author":"S Xu","year":"2020","unstructured":"Xu S, Lu J, Shao A, Zhang JH, Zhang J (2020) Glial cells: role of the immune response in ischemic stroke. Front Immunol 11:294. https:\/\/doi.org\/10.3389\/fimmu.2020.00294","journal-title":"Front Immunol"},{"issue":"10","key":"1103_CR50","doi-asserted-by":"publisher","first-page":"1042","DOI":"10.1038\/ajh.2012.67","volume":"25","author":"T Yang","year":"2012","unstructured":"Yang T, Du Y (2012) Distinct roles of central and peripheral prostaglandin E2 and EP subtypes in blood pressure regulation. Am J Hypertens 25(10):1042\u20131049. https:\/\/doi.org\/10.1038\/ajh.2012.67","journal-title":"Am J Hypertens"},{"key":"1103_CR51","doi-asserted-by":"publisher","first-page":"811","DOI":"10.3389\/fnins.2018.00811","volume":"12","author":"AM Zagrean","year":"2018","unstructured":"Zagrean AM, Hermann DM, Opris I, Zagrean L, Popa-Wagner A (2018) Multicellular crosstalk between exosomes and the neurovascular unit after cerebral ischemia. Therapeutic Implications Front Neurosci 12:811. https:\/\/doi.org\/10.3389\/fnins.2018.00811","journal-title":"Therapeutic Implications Front Neurosci"}],"container-title":["Cellular and Molecular Neurobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10571-021-01103-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10571-021-01103-5\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10571-021-01103-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,8,26]],"date-time":"2022-08-26T14:16:42Z","timestamp":1661523402000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10571-021-01103-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,25]]},"references-count":51,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2022,10]]}},"alternative-id":["1103"],"URL":"https:\/\/doi.org\/10.1007\/s10571-021-01103-5","relation":{},"ISSN":["0272-4340","1573-6830"],"issn-type":[{"value":"0272-4340","type":"print"},{"value":"1573-6830","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,25]]},"assertion":[{"value":"6 January 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 May 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"25 May 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare that they have no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"The animals were used in accordance with the national ethical requirements for animal research and with the European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes (Directive 2010\/63\/EU). All animal experiments were approved by the Animal Research Committee of University of Beira Interior (CICS-UBI, Covilh\u00e3, Portugal).","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical Approval"}}]}}