{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T07:56:08Z","timestamp":1772006168423,"version":"3.50.1"},"reference-count":63,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2011,2,24]],"date-time":"2011-02-24T00:00:00Z","timestamp":1298505600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Sol-Gel Sci Technol"],"published-print":{"date-parts":[[2011,5]]},"DOI":"10.1007\/s10971-011-2426-7","type":"journal-article","created":{"date-parts":[[2011,2,24]],"date-time":"2011-02-24T03:04:14Z","timestamp":1298516654000},"page":"545-556","source":"Crossref","is-referenced-by-count":24,"title":["Optimization in the immobilization of penicillin G acylase by entrapment in xerogel particles with magnetic properties"],"prefix":"10.1007","volume":"58","author":[{"given":"Susana","family":"Bernardino","sequence":"first","affiliation":[]},{"given":"N\u00eddia","family":"Estrela","sequence":"additional","affiliation":[]},{"given":"Vanessa","family":"Ochoa-Mendes","sequence":"additional","affiliation":[]},{"given":"Pedro","family":"Fernandes","sequence":"additional","affiliation":[]},{"given":"Lu\u00eds P.","family":"Fonseca","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2011,2,24]]},"reference":[{"key":"2426_CR1","volume-title":"Handbook of enzyme biotechnology","author":"PSJ Cheetham","year":"1995","unstructured":"Cheetham PSJ (1995) The application of enzymes in industry. In: Wiseman A, Horwood Ellis (eds) Handbook of enzyme biotechnology. Wiley, London"},{"key":"2426_CR2","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1016\/S0958-1669(02)00360-9","volume":"13","author":"AJJ Straathof","year":"2002","unstructured":"Straathof AJJ, Panke S, Schmid A (2002) The production of fine chemicals by biotransformations. Curr Opin Biotechnol 13:548\u2013556","journal-title":"Curr Opin Biotechnol"},{"key":"2426_CR3","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.biotechadv.2005.05.003","volume":"24","author":"RC Giordano","year":"2006","unstructured":"Giordano RC, Ribeiro MPA, Giordano RLC (2006) Kinetics of \u03b2-lactam antibiotics synthesis by penicillin G acylase (PGA) from the viewpoint of the industrial enzymatic reactor optimization. Biotecchnol Adv 24:27\u201341","journal-title":"Biotecchnol Adv"},{"key":"2426_CR4","doi-asserted-by":"crossref","first-page":"1710","DOI":"10.1002\/jctb.1992","volume":"83","author":"X Jin","year":"2008","unstructured":"Jin X, Wu Q, Chen Q, Chen C-X, Lin X-F (2008) Immobilization of penicillin G acylase on a composite carrier with a biocompatible microenvironment of chitosan. J Chem Techonol Biotechnol 83:1710\u20131716","journal-title":"J Chem Techonol Biotechnol"},{"key":"2426_CR5","first-page":"146","volume":"24","author":"JG Shewale","year":"1989","unstructured":"Shewale JG, Sivaraman H (1989) Penicillin acylase: enzyme production and its application in the manufacture of 6-APA. Process Biochem 24:146\u2013154","journal-title":"Process Biochem"},{"key":"2426_CR6","doi-asserted-by":"crossref","first-page":"1807","DOI":"10.1021\/la803480c","volume":"25","author":"H Sun","year":"2009","unstructured":"Sun H, Bao XY, Zhao XS (2009) Immobilization of penicillin G acylase on oxirane-modified mesoporous silicas. Langmuir 25:1807\u20131812","journal-title":"Langmuir"},{"key":"2426_CR7","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1002\/bit.20728","volume":"93","author":"L Serenovic","year":"2006","unstructured":"Serenovic L, Stankovic N, Spizzo P, Basso A et al (2006) Highlevel production and covalent immobilization of Providencia rettgeri Penicillin G Acylase (PAC) from recombinant Pichia pastoris for the development of a novel and stable biocatalyst of industrial applicability. Biotechnol Bioeng 93:344\u2013354","journal-title":"Biotechnol Bioeng"},{"key":"2426_CR8","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1016\/j.jmb.2005.03.005","volume":"348","author":"Z Ignatova","year":"2005","unstructured":"Ignatova Z, Wischnewski F, Notbohm H, Kasche V (2005) Pro-sequence and Ca2+ -binding: implications for folding and maturation of Ntn-hydrolase Penicillin Amidase from E. coli. J Mol Biol 348:999\u20131014","journal-title":"J Mol Biol"},{"key":"2426_CR9","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1038\/373264a0","volume":"373","author":"HJ Duggleby","year":"1995","unstructured":"Duggleby HJ, Tolley SP, Hill CP, Dodson EJ, Dodson G, Moody PCE (1995) Penicillin acylase has a single amino acid catalytic centre. Nature 373:264\u2013268","journal-title":"Nature"},{"key":"2426_CR10","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1007\/s00253-002-1113-6","volume":"60","author":"M Arroyo","year":"2003","unstructured":"Arroyo M, de la Mata I, Acebal C (2003) Biotechnological applications of penicillin acylases: state-of-the-art. Appl Microbiol Biotechnol 60:507\u2013514","journal-title":"Appl Microbiol Biotechnol"},{"key":"2426_CR11","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.molcatb.2004.01.020","volume":"30","author":"A Illanes","year":"2004","unstructured":"Illanes A (2004) Optimization of cephalexin synthesis with immobilized penicillin acylase in ethylene glycol medium at low temperatures. J Mol Catal B Enzym 30:95\u2013103","journal-title":"J Mol Catal B Enzym"},{"key":"2426_CR12","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1002\/adsc.200700082","volume":"349","author":"RA Sheldon","year":"2007","unstructured":"Sheldon RA (2007) Enzyme immobilization: the quest for optimum performance. Adv Synth Catal 349:1289\u20131307","journal-title":"Adv Synth Catal"},{"key":"2426_CR13","doi-asserted-by":"crossref","first-page":"1451","DOI":"10.1016\/j.enzmictec.2007.01.018","volume":"40","author":"C Mateo","year":"2007","unstructured":"Mateo C, Palomo JM, Fernandez-Lorente G, Guisan JM, Fernandez-Lafuente R (2007) Improvement of enzyme activity, stability and selectivity via immobilization techniques. Enz Microb Technol 40:1451\u20131463","journal-title":"Enz Microb Technol"},{"key":"2426_CR14","unstructured":"Cabral JMS, Kennedy JF (1993). In: Gupta MN (ed). Thermostability of enzymes. Springer, Berlin"},{"key":"2426_CR15","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/S1381-1177(01)00020-0","volume":"15","author":"CGPH Schro\u00ebn","year":"2001","unstructured":"Schro\u00ebn CGPH, Eldin MSM, Janssen AEM, Mita GD, Tramper J (2001) Cephalexin synthesis by immobilised penicillin G acylase under non-isothermal conditions: reduction of diffusion limitation. J Mol Catal B Enzym 15:163\u2013172","journal-title":"J Mol Catal B Enzym"},{"issue":"3\/4","key":"2426_CR16","first-page":"141","volume":"23","author":"AI Kallenberg","year":"2005","unstructured":"Kallenberg AI, van Rantwijk F, Sheldon RA (2005) Immobilization of penicillin G acylase: the-linked enzyme aggregates (CLEAs): a novel and versatile method for enzyme immobilization (a review). Biocatal Biotrandformation 23(3\/4):141\u2013147","journal-title":"Biocatal Biotrandformation"},{"key":"2426_CR17","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.molcatb.2006.07.001","volume":"43","author":"HW Yu","year":"2006","unstructured":"Yu HW, Chen H, Wang X, Yang YY, Ching CB (2006) Cross-linked enzyme aggregates (CLEAs) with controlled particles: application to Candida rugosa lipase. J Mol Catal B Enzym 43:124\u2013127","journal-title":"J Mol Catal B Enzym"},{"key":"2426_CR18","volume-title":"Recent advances in biocatalysis and biotransformation","author":"L Betancor","year":"2007","unstructured":"Betancor L, Luckarift HR (2007) Biosilica-immobilized enzymes for biocatalysis. In: Palomo JM (ed) Recent advances in biocatalysis and biotransformation. Research Signpost, Florida"},{"issue":"3\/4","key":"2426_CR19","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1080\/10242420500183378","volume":"23","author":"RA Sheldon","year":"2005","unstructured":"Sheldon RA, Schoevaart R, Van Langen LM (2005) Cross-linked enzyme aggregates (CLEAs): a novel and versatile method for enzyme immobilization (a review). Biocatal Biotransform 23(3\/4):141\u2013147","journal-title":"Biocatal Biotransform"},{"issue":"5","key":"2426_CR20","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1002\/bit.20107","volume":"86","author":"L Wilson","year":"2004","unstructured":"Wilson L, Illanes A et al (2004) Encapsulation of crosslinked penicillin G acylase aggregates in lentikats: evaluation of a novel biocatalyst in organic media. Biotechnol Bioeng 86(5):558\u2013562","journal-title":"Biotechnol Bioeng"},{"key":"2426_CR21","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1021\/cm00046a008","volume":"5","author":"D Avnir","year":"1994","unstructured":"Avnir D, Braun S, Lev O, Ottolenghi M (1994) Enzymes and other proteins entrapped in sol-gel materials. Chem Mater 5:1605\u20131614","journal-title":"Chem Mater"},{"key":"2426_CR22","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/S1359-0286(97)80057-5","volume":"2","author":"J Livage","year":"1997","unstructured":"Livage J (1997) Sol-gel processes. Curr Opin Solid State Mater Sci 2:132\u2013138","journal-title":"Curr Opin Solid State Mater Sci"},{"key":"2426_CR23","doi-asserted-by":"crossref","first-page":"R673","DOI":"10.1088\/0953-8984\/13\/33\/202","volume":"13","author":"J Livage","year":"2001","unstructured":"Livage J, Coradin T, Roux C (2001) Encapsulation of biomolecules in silica gels. J Phys Condens Matter 13:R673\u2013R691","journal-title":"J Phys Condens Matter"},{"key":"2426_CR24","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1007\/BF02931080","volume":"12","author":"MJ Shin","year":"2007","unstructured":"Shin MJ, Park JY, Park K, Song S, Yoo YJ (2007) Novel sol-gel immobilization of horseradish peroxidase employing a detergentless micro-emulsion system. Biotechnol Bioprocess Eng 12:640\u2013645","journal-title":"Biotechnol Bioprocess Eng"},{"key":"2426_CR25","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1023\/A:1015572020633","volume":"24","author":"H O\u2032Neill","year":"2002","unstructured":"O\u2032Neill H, Angley CV, Hemery I, Evans BR, Dai S, Woodward J (2002) Properties of carbohydrate-metabolizing enzymes immobilized in sol-gel beads: stabilization of invertase and \u03b2-glucosidase by blue dextran. Biotechnol Lett 24:783\u2013790","journal-title":"Biotechnol Lett"},{"key":"2426_CR26","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/S1381-1169(98)00043-0","volume":"134","author":"MT Reetz","year":"1998","unstructured":"Reetz MT, Zonta A, Vijayakrishnan V, Schimossek K (1998) Entrapment of lipases in hydrophobic magnetite-containing sol-gel materials: magnetic separation of heterogeneous biocatalysts. J Mol Catal A Chem 134:251\u2013258","journal-title":"J Mol Catal A Chem"},{"key":"2426_CR27","volume-title":"Sol-gel science: the physics and chemistry of sol-gel processing","author":"JC Brinker","year":"1990","unstructured":"Brinker JC, Scherer GW (1990) Sol-gel science: the physics and chemistry of sol-gel processing. Book News Inc, Portland"},{"key":"2426_CR28","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1007\/BF00159232","volume":"9","author":"K Kawakami","year":"1995","unstructured":"Kawakami K, Yoshida S (1995) Sol-gel entrapment of lipase using a mixture of tetramethoxisilane and methyltrimethoxysilane as the alkoxide precursor: esterification activity in organic media. Biotechnol Tech 9:701\u2013704","journal-title":"Biotechnol Tech"},{"key":"2426_CR29","doi-asserted-by":"crossref","first-page":"1953","DOI":"10.1023\/A:1026702025928","volume":"22","author":"B Li","year":"2000","unstructured":"Li B, Takahashi H (2000) New immobilization method for enzyme stabilization involving a mesoporous material and an organic\/inorganic hybrid gel. Biotechnol Lett 22:1953\u20131958","journal-title":"Biotechnol Lett"},{"key":"2426_CR30","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0003-2670(02)00229-5","volume":"461","author":"W Jin","year":"2002","unstructured":"Jin W, Brennan JD (2002) Properties and applications of proteins encapsulated within sol-gel derived materials. Anal Chim Acta 461:1\u201336","journal-title":"Anal Chim Acta"},{"key":"2426_CR31","doi-asserted-by":"crossref","first-page":"11289","DOI":"10.1021\/bi0155504","volume":"40","author":"HX Zhou","year":"2001","unstructured":"Zhou HX, Dill KA (2001) Stabilization of proteins in confined spaces. Biochemistry 40:11289\u201311293","journal-title":"Biochemistry"},{"key":"2426_CR32","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1021\/bm0493077","volume":"6","author":"L Betancor","year":"2005","unstructured":"Betancor L, L\u00f3pez-Gallego F, Hidalgo A, Fuentesm M, Podrasky O, Kuncova G, Guis\u00e1n JM, Fernandez-Lafuente R (2005) Advantages of the pre-immobilization of enzymes on porous supports for their entrapment in sol-gels. Biomacromolecules 6:1027\u20131030","journal-title":"Biomacromolecules"},{"key":"2426_CR33","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1016\/S0167-7799(00)01457-8","volume":"18","author":"I Gill","year":"2000","unstructured":"Gill I, Ballesteros A (2000) Bioencapsulation within synthetic polymers (part 1): sol-gel encapsulated biological. Trends Biotechnol 18:282\u2013296","journal-title":"Trends Biotechnol"},{"key":"2426_CR34","doi-asserted-by":"crossref","first-page":"8627","DOI":"10.1016\/S0040-4039(00)01522-7","volume":"41","author":"A Basso","year":"2000","unstructured":"Basso A, De Martin L, Ebert C, Gardossi L, Tomat A, Casarci M, Rosi OL (2000) A novel support for enzyme adsorption: properties and applications of aerogels in low water media. Tetrahedrom Lett 41:8627\u20138630","journal-title":"Tetrahedrom Lett"},{"key":"2426_CR35","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1039\/B512706H","volume":"16","author":"D Avnir","year":"2006","unstructured":"Avnir D, Coradin T, Lev O, Livage J (2006) Recent bio-applications of sol-gel materials. J Mater Chem 16:1013\u20131030","journal-title":"J Mater Chem"},{"key":"2426_CR36","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.nantod.2009.02.001","volume":"4","author":"C-H Lee","year":"2009","unstructured":"Lee C-H, Lin T-S, Mou C-Y (2009) Mesoporous materials for encapsulating enzymes. Nano Today 4:165\u2013179","journal-title":"Nano Today"},{"key":"2426_CR37","first-page":"583","volume":"42","author":"MF Desimone","year":"2008","unstructured":"Desimone MF, Matiacevich SB, Buera MP, Diaz LE (2008) Effects of relative humidity on enzyme activity immobilizedin sol\u2013gel-derived silica nanocomposites. Technology 42:583\u2013588","journal-title":"Technology"},{"key":"2426_CR38","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/S0167-577X(99)00287-6","volume":"44","author":"Y Wei","year":"2000","unstructured":"Wei Y, Xu J, Fen Q, Dong H, Lin M (2000) Encapsulation of enzymes in mesoporous host materials via the nonsurfactant-templated sol-gel process. Mater Lett 44:6\u201311","journal-title":"Mater Lett"},{"key":"2426_CR39","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1007\/BF03026240","volume":"11","author":"L Chang-won","year":"2006","unstructured":"Chang-won L, Yi S, Kim J, Lee Y, Kim B (2006) Improved immobilized enzyme systems using spherical micro silica sol-gel enzyme beads. Biotechnol Bioprocess Eng 11:277\u2013281","journal-title":"Biotechnol Bioprocess Eng"},{"key":"2426_CR40","doi-asserted-by":"crossref","first-page":"1504","DOI":"10.1246\/cl.2004.1504","volume":"33","author":"K Adachi","year":"2004","unstructured":"Adachi K, Iwamura T, Chujo Y (2004) Novel synthesis of submicrometer silica spheres in non-alcoholic solvent by microwave-assisted sol-gel method. Chem Lett 33:1504\u20131505","journal-title":"Chem Lett"},{"key":"2426_CR41","doi-asserted-by":"crossref","first-page":"3522","DOI":"10.1016\/j.apsusc.2007.11.048","volume":"254","author":"J Dong","year":"2008","unstructured":"Dong J, Xu Z, Wang F (2008) Engineering and characterization of mesoporous silica-coated magnetic particles for mercury removal from industrial effluents. Appl Surf Sci 254:3522\u20133530","journal-title":"Appl Surf Sci"},{"key":"2426_CR42","doi-asserted-by":"crossref","first-page":"2544","DOI":"10.1021\/cm703208w","volume":"20","author":"R Abu-Reziq","year":"2008","unstructured":"Abu-Reziq R, Wang D, Post M, Alper H (2008) Separable catalysts in one-pot synthesis for greener chemistry. Chem Mater 20:2544\u20132550","journal-title":"Chem Mater"},{"key":"2426_CR43","doi-asserted-by":"crossref","unstructured":"Yilmaz E, Sezgin M and Yilmaz M (2011) Immobilization of Candida rugosa lipase on magnetic sol\u2013gel composite supports for enzymatic resolution of (R,S)-Naproxen methyl ester. J Mol Catal B Enzym. doi: 10.1016\/j.molcatb.2010.12.007","DOI":"10.1016\/j.molcatb.2010.12.007"},{"key":"2426_CR44","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1007\/s10971-007-1648-1","volume":"45","author":"Y Kobayashi","year":"2008","unstructured":"Kobayashi Y, Saeki S, Yoshida M, Nagao D, Konno M (2008) Synthesis of spherical submicron-sized magnetite\/silica nanocomposite particles. J Sol-Gel Technolol 45:35\u201341","journal-title":"J Sol-Gel Technolol"},{"key":"2426_CR45","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1002\/biot.200800287","volume":"4","author":"SMSA Bernardino","year":"2009","unstructured":"Bernardino SMSA, Fernandes P, Fonseca LP (2009) A new biocatalyst: penicillin G acylase immobilized in sol-gel micro-particles with magnetic properties. Biotechnol J 4:695\u2013702","journal-title":"Biotechnol J"},{"issue":"1","key":"2426_CR46","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1038\/nprot.2006.4","volume":"1","author":"H Sch\u00e4gger","year":"2006","unstructured":"Sch\u00e4gger H (2006) Tricine-SDS-PAGE. Nat Protoc 1(1):16\u201322","journal-title":"Nat Protoc"},{"issue":"4","key":"2426_CR47","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1002\/1097-0290(20010220)72:4<475::AID-BIT1009>3.0.CO;2-F","volume":"72","author":"YD Kim","year":"2001","unstructured":"Kim YD, Dordick JS, Clark DS (2001) Siloxane-based biocatalytic films and paints for use as reactive coatings. Biotechnol Bioeng 72(4):475\u2013482","journal-title":"Biotechnol Bioeng"},{"key":"2426_CR48","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1126\/science.1312257","volume":"255","author":"LM Ellerby","year":"1992","unstructured":"Ellerby LM, Nishida CR, Nishida F, Yamanaka SA, Dunn B, Valentine JS, Zink JI (1992) Encapsulation of proteins in transparent porous silicate glasses prepared by the sol-gel method. Science 255:1113\u20131115","journal-title":"Science"},{"key":"2426_CR49","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1002\/jctb.280580105","volume":"58","author":"LP Fonseca","year":"1993","unstructured":"Fonseca LP, Cardoso JP, Cabral JMS (1993) Immobilization studies of an industrial penicillin acylase preparation on a silica carrier. J Chem Tech Biotechnol 58:27\u201337","journal-title":"J Chem Tech Biotechnol"},{"key":"2426_CR50","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/0003-2697(85)90442-7","volume":"150","author":"PK Smith","year":"1985","unstructured":"Smith PK et al (1985) Measurements of protein using bicinhoninic acid. Anal Biochem 150:76\u201385","journal-title":"Anal Biochem"},{"issue":"6","key":"2426_CR51","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1590\/S0100-40421998000600020","volume":"21","author":"DAM Zaia","year":"1998","unstructured":"Zaia DAM, Zaia CTBV, Lichtig J (1998) Determina\u00e7\u00e3o de Prote\u00ednas Totais via especrofotometria: vantagens e desvantagens dos m\u00e9todos existentes. Quim Nova 21(6):787\u2013793","journal-title":"Quim Nova"},{"issue":"3","key":"2426_CR52","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1080\/10242420412331283314","volume":"22","author":"AC Pierre","year":"2004","unstructured":"Pierre AC (2004) The sol-gel encapsulation of enzymes. Biocatal Biotransform 22(3):145\u2013170","journal-title":"Biocatal Biotransform"},{"key":"2426_CR53","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1002\/bit.1066","volume":"73","author":"Y-D Kim","year":"2001","unstructured":"Kim Y-D, Park CB, Clark DS (2001) Stable sol-gel microstructured and microfluidic networks for protein patterning. Biotechnol Bioeng 73:331\u2013337","journal-title":"Biotechnol Bioeng"},{"key":"2426_CR54","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/0922-338X(96)88814-3","volume":"82","author":"K Kawakami","year":"1996","unstructured":"Kawakami K, Yoshida S (1996) Thermal stabilization of lipase by sol-gel entrapment modified silicates formed on kieselguhr. J Ferment Bioeng 82:239\u2013245","journal-title":"J Ferment Bioeng"},{"issue":"2","key":"2426_CR55","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1002\/bit.22142","volume":"102","author":"LM Bergeron","year":"2008","unstructured":"Bergeron LM, Tokatlian T, Gomez L, Clark DS (2008) Redirecting the inactivation pathway of penicillin amidase and increasing amoxicillin production via a thermophilic molecular chaperone. Biotechnol Bioeng 102(2):417\u2013424","journal-title":"Biotechnol Bioeng"},{"issue":"1","key":"2426_CR56","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1080\/10242420210158","volume":"20","author":"M Arroyo","year":"2002","unstructured":"Arroyo M, Torres-Guzm\u00e1n R, de la Mata I, Castill\u00f3n MP, Acebal C (2002) A kinetic examination of penicillin acylase stability in water-organic solvent systems at different temperatures. Biocatal Biotransform 20(1):53\u201356","journal-title":"Biocatal Biotransform"},{"key":"2426_CR57","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/0032-9592(95)80003-4","volume":"30","author":"A Erarslan","year":"1994","unstructured":"Erarslan A (1994) Effect of polyol compounds on thermostability of penicillin G acylase obtained from a mutant of Escherichia coli ATCC 11105. Process Biochem 30:133\u2013139","journal-title":"Process Biochem"},{"key":"2426_CR58","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1023\/A:1008774827602","volume":"14","author":"DL Meixner","year":"1999","unstructured":"Meixner DL, Dyer PN (1999) Influence of sol-gel synthesis parameters on the microstructure of particulate silica xerogels. J Sol-Gel Sci Technol 14:223\u2013232","journal-title":"J Sol-Gel Sci Technol"},{"issue":"1","key":"2426_CR59","doi-asserted-by":"crossref","first-page":"89","DOI":"10.6028\/jres.081A.011","volume":"81A","author":"L Greenspan","year":"1977","unstructured":"Greenspan L (1977) Humidity fixed points of binary saturated aqueous solutions. J Res Natl Bureau Standards-A Phys Chem 81A(1):89\u201396","journal-title":"J Res Natl Bureau Standards-A Phys Chem"},{"key":"2426_CR60","doi-asserted-by":"crossref","first-page":"932","DOI":"10.1039\/B611171H","volume":"36","author":"M Pagliaro","year":"2007","unstructured":"Pagliaro M, Ciriminna R, Palmisano G (2007) The chemical effects of molecular sol\u2013gel entrapment. Chem Soc Rev 36:932\u2013940","journal-title":"Chem Soc Rev"},{"key":"2426_CR61","unstructured":"Ramakrishna SV, Sreedharan VP, Prema P (1988) In: Moo-Young M (ed) Bioreactor immobilized enzymes and cells. Elsevier Publishers, Amsterdam"},{"key":"2426_CR62","doi-asserted-by":"crossref","first-page":"8587","DOI":"10.1021\/ja9814568","volume":"120","author":"I Gill","year":"1998","unstructured":"Gill I, Ballesteros A (1998) Encapsulation of biologicals within silicate, siloxane, and hybrid sol-gel polymers: an efficient and generic approach. J Am Chem Soc 120:8587\u20138598","journal-title":"J Am Chem Soc"},{"key":"2426_CR63","doi-asserted-by":"crossref","first-page":"6292","DOI":"10.1021\/cm8012107","volume":"20","author":"L Li","year":"2008","unstructured":"Li L, Choo ESG, Yi J, Ding J, Tang X, Xue J (2008) Superparamagnetic silica composite nanospheres (SSCNs) with ultrahigh loading of iron oxide nanoparticles via an oil-in-DEG microemulsion route. Chem Mater 20:6292\u20136294","journal-title":"Chem Mater"}],"container-title":["Journal of Sol-Gel Science and Technology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10971-011-2426-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s10971-011-2426-7\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10971-011-2426-7","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,6,8]],"date-time":"2019-06-08T20:01:33Z","timestamp":1560024093000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s10971-011-2426-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,2,24]]},"references-count":63,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2011,5]]}},"alternative-id":["2426"],"URL":"https:\/\/doi.org\/10.1007\/s10971-011-2426-7","relation":{},"ISSN":["0928-0707","1573-4846"],"issn-type":[{"value":"0928-0707","type":"print"},{"value":"1573-4846","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,2,24]]}}}