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Medicago cells were collected on day 7 of the growth curve, which corresponded to the beginning of the exponential phase. They were then co-cultured with Agrobacterium for 3\u00a0days before being spread onto a petri dish with appropriate antibiotic selection. The Receptor Binding Domain of the Spike protein of SARS-CoV-2 was used as a model to develop this protocol. The presence of the transgene was assessed using PCR, and the integrity of the product was evaluated by SDS-PAGE and Western-blotting.<\/jats:p>","DOI":"10.1007\/s11240-023-02495-6","type":"journal-article","created":{"date-parts":[[2023,3,23]],"date-time":"2023-03-23T08:03:28Z","timestamp":1679558608000},"page":"669-675","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["A simplified protocol for Agrobacterium-mediated transformation of cell suspension cultures of the model species Medicago truncatula A17"],"prefix":"10.1007","volume":"153","author":[{"given":"Ana Clara","family":"Ferreira","sequence":"first","affiliation":[]},{"given":"B\u00e1rbara A.","family":"Rebelo","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3173-3701","authenticated-orcid":false,"given":"Rita","family":"Abranches","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2023,3,23]]},"reference":[{"key":"2495_CR1","doi-asserted-by":"publisher","first-page":"568","DOI":"10.1104\/pp.79.2.568","volume":"79","author":"G An","year":"1985","unstructured":"An G (1985) High efficiency transformation of cultured tobacco cells. 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