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Following cooled PBS washing, we observed a significant increase in p32 levels and in the proportion of dead spermatozoa with compromised acrosomal integrity compared to sperm washing using PBS at room temperature. Interestingly, this increase in p32 was effectively inhibited when cooled PBS was supplemented with 1 mM AEBSF, a serine protease inhibitor. Our findings suggest that the increase of p32 in response to cooled PBS washing in boar spermatozoa is associated with enhanced protease activity in dead spermatozoa.<\/jats:p>","DOI":"10.1007\/s11259-023-10247-2","type":"journal-article","created":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T00:01:40Z","timestamp":1698364900000},"page":"1189-1193","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Processing of boar spermatozoa with phosphate-buffered saline at 4\u02daC induces an increase in 32\u00a0kDa tyrosine-phosphorylated protein (p32)"],"prefix":"10.1007","volume":"48","author":[{"given":"David","family":"Mart\u00edn-Hidalgo","sequence":"first","affiliation":[]},{"given":"Mercedes","family":"Izquierdo","sequence":"additional","affiliation":[]},{"given":"Paloma","family":"Bartolom\u00e9-Garc\u00eda","sequence":"additional","affiliation":[]},{"given":"Beatriz","family":"Mac\u00edas-Garc\u00eda","sequence":"additional","affiliation":[]},{"given":"Lauro","family":"Gonz\u00e1lez-Fern\u00e1ndez","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2023,10,27]]},"reference":[{"issue":"4","key":"10247_CR1","doi-asserted-by":"publisher","first-page":"581","DOI":"10.1071\/bi9510581","volume":"4","author":"CR Austin","year":"1951","unstructured":"Austin CR (1951) Observations on the penetration of the sperm in the mammalian egg. 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