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In the northeast region of Portugal, infection with <jats:italic>Brucella melitensis<\/jats:italic> is endemic in small ruminants, and there are also humans\u2019 cases. However, the epidemiological role of the wild boar in the dynamics of this disease in this region is unknown. In this study, a total of 332 blood samples were collected from wild boar hunted in thirty-six hunting areas during the 2022\/2023 hunting season. All were taken by the hunters for private consumption, with no evisceration or examination in the field. Serum samples were tested by indirect ELISA (i-ELISA). It was observed that 88 wild boars were exposed to <jats:italic>Brucella<\/jats:italic> spp., pointing to a seroprevalence of 26.5% (95% CI: 21.8 \u2013 31.3%). This high prevalence underlines the importance that wild boar may have in the dynamics of this disease in the region and its potential transmission to other animals, and to humans (for example, during the handling of carcasses). 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No ethical approval was deemed necessary.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Statement of animal rights"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"The authors declare no conflict of interest.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}