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The Minimal Information for Studies of Extracellular Vesicles 2018 (MISEV2018) guidelines were published to improve rigor and standardisation within the EV field and provide a framework for the reliable isolation and characterisation of EV populations. However, this rigor and standardisation has been challenging in the area of comparative medicine. Herein we present the successful isolation of EVs from human and canine plasma using Size Exclusion Chromatography and characterise these EVs according to best international practice. This study provides evidence for the reliable comparison of human and canine EVs isolated by this approach, and a baseline description of the EVs from healthy dogs to inform future biomarker studies. This work also demonstrates that the MISEV2018 guidelines can be successfully applied to EVs isolated from canine plasma.<\/jats:p>","DOI":"10.1007\/s11259-024-10405-0","type":"journal-article","created":{"date-parts":[[2024,5,8]],"date-time":"2024-05-08T04:01:38Z","timestamp":1715140898000},"page":"2775-2782","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Comparative characterisation of extracellular vesicles from canine and human plasma: a necessary step in biomarker discovery"],"prefix":"10.1007","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3220-6903","authenticated-orcid":false,"given":"Stephanie Marie","family":"Bollard","sequence":"first","affiliation":[]},{"given":"J.","family":"Howard","sequence":"additional","affiliation":[]},{"given":"C.","family":"Casalou","sequence":"additional","affiliation":[]},{"given":"L.","family":"Mooney","sequence":"additional","affiliation":[]},{"given":"S.","family":"Peters","sequence":"additional","affiliation":[]},{"given":"C.","family":"Sweeney","sequence":"additional","affiliation":[]},{"given":"A.","family":"Ajaykumar","sequence":"additional","affiliation":[]},{"given":"K.","family":"Triana","sequence":"additional","affiliation":[]},{"given":"A.","family":"McCann","sequence":"additional","affiliation":[]},{"given":"P. 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Approval for the collection and analysis of human samples was granted by the Institutional Review Board at the Mater Misericordiae University Hospital, Dublin Ireland (Ref: 1\/378\/2189). Informed consent was obtained from the human participants included in the study. The use of canine samples for this study was granted an ethical exemption by the Animal Research Ethics Committee at University College Dublin, Ireland (Ref: AREC-E-20-26-Kelly).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}