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Their role in the ecology of emerging infectious diseases continues to be of significant interest. This study aimed to evaluate the occurrence of coronaviruses (CoVs) in Portuguese bats and predict the affinity of their spike proteins with the aminopeptidase N (APN) receptor of several host species. The study also explored the viral diversity in bat samples using metagenomic sequencing. Ten bats (five\n                    <jats:italic>Myotis myotis<\/jats:italic>\n                    and five\n                    <jats:italic>Miniopterus schreibersii<\/jats:italic>\n                    ) were captured at an underground roost in 2022 (Central Portugal), and fecal samples, oral, and anal swabs were collected (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200927). A Pan-CoV nested RT-PCR was used for initial screening, followed by viral metagenomic sequencing of all fecal samples and one CoV-positive buccal swab. In silico protein docking studies were performed between a Portuguese bat CoV spike protein and APNs of bats, pigs, and humans. Pan-CoV nested RT-PCR identified three positive samples: two fecal samples and one buccal sample. Metagenomic sequencing allowed us to determine two near complete CoV genomes. Protein docking predicted strong binding of this spike protein to bat, porcine, and human APN receptors. Metagenomics also identified picornaviruses, adenovirus, and dependoparvovirus in fecal samples. This study reports the first near complete genome sequences of two members of the\n                    <jats:italic>Alphacoronavirus<\/jats:italic>\n                    genus from a Portuguese bat The identification of other viral families highlights the diverse virome of these cave-dwelling bat species. Protein docking studies suggest a potential for cross-species transmission of this bat CoV between bats, porcines and humans, though further research is needed to confirm these interactions.\n                  <\/jats:p>","DOI":"10.1007\/s11259-025-10888-5","type":"journal-article","created":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T03:10:37Z","timestamp":1758337837000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Metagenomic analysis of viral diversity in Portuguese bats"],"prefix":"10.1007","volume":"49","author":[{"given":"Mahima","family":"Hemnani","sequence":"first","affiliation":[]},{"given":"Mustafa","family":"Karatas","sequence":"additional","affiliation":[]},{"given":"Andreia V S","family":"Cruz","sequence":"additional","affiliation":[]},{"given":"Priscilla Gomes","family":"da Silva","sequence":"additional","affiliation":[]},{"given":"Gertrude","family":"Thompson","sequence":"additional","affiliation":[]},{"given":"Patr\u00edcia","family":"Poeta","sequence":"additional","affiliation":[]},{"given":"Hugo","family":"Rebelo","sequence":"additional","affiliation":[]},{"given":"Jelle","family":"Matthijnssens","sequence":"additional","affiliation":[]},{"given":"Jo\u00e3o R","family":"Mesquita","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2025,9,20]]},"reference":[{"key":"10888_CR1","doi-asserted-by":"publisher","first-page":"271","DOI":"10.1002\/prot.22170","volume":"73","author":"N Andrusier","year":"2008","unstructured":"Andrusier N, Mashiach E, Nussinov R, Wolfson HJ (2008) Principles of flexible protein-protein docking. 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