{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,3]],"date-time":"2022-04-03T06:53:33Z","timestamp":1648968813482},"reference-count":27,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2011,9,25]],"date-time":"2011-09-25T00:00:00Z","timestamp":1316908800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tree Genetics &amp; Genomes"],"published-print":{"date-parts":[[2012,4]]},"DOI":"10.1007\/s11295-011-0434-y","type":"journal-article","created":{"date-parts":[[2011,9,24]],"date-time":"2011-09-24T10:34:06Z","timestamp":1316860446000},"page":"425-430","source":"Crossref","is-referenced-by-count":6,"title":["Determining DNA strand breakage from embryogenic cell cultures of a conifer species using the single-cell gel electrophoresis assay"],"prefix":"10.1007","volume":"8","author":[{"given":"Pedro M.","family":"Costa","sequence":"first","affiliation":[]},{"given":"Ana","family":"Milhinhos","sequence":"additional","affiliation":[]},{"given":"Marta","family":"Sim\u00f5es","sequence":"additional","affiliation":[]},{"given":"Liliana","family":"Marum","sequence":"additional","affiliation":[]},{"given":"Ana Maria","family":"Oliveira","sequence":"additional","affiliation":[]},{"given":"Maria H.","family":"Costa","sequence":"additional","affiliation":[]},{"given":"C\u00e9lia","family":"Miguel","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2011,9,25]]},"reference":[{"key":"434_CR1","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/S1383-5718(01)00327-8","volume":"514","author":"B Ateeq","year":"2002","unstructured":"Ateeq B, Farah MA, Ali MN, Ahmad W (2002) Clastogenicity of pentachlorophenol, 2,4-D and butachlor evaluated by Allium root tip test. Mutat Res 514:105\u2013113","journal-title":"Mutat Res"},{"key":"434_CR2","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1016\/0304-4211(76)90162-0","volume":"7","author":"MS Banks","year":"1976","unstructured":"Banks MS, Evans PK (1976) A comparison of the isolation and culture of mesophyll protoplasts from several Nicotiana species and their hybrids. Plant Sci Lett 7:409\u2013416","journal-title":"Plant Sci Lett"},{"key":"434_CR3","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1093\/treephys\/1.2.227","volume":"1","author":"GP Berlyn","year":"1986","unstructured":"Berlyn GP, Beck RC, Renfroe MH (1986) Tissue culture and the propagation and genetic improvement of conifers: problems and possibilities. Tree Physiol 1:227\u2013240","journal-title":"Tree Physiol"},{"key":"434_CR4","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1034\/j.1399-3054.1998.1040209.x","volume":"104","author":"PV Bozhkov","year":"1998","unstructured":"Bozhkov PV, von Arnold S (1998) Polyethylene glycol promotes maturation but inhibits further development of Picea abies somatic embryos. Physiol Plant 104:211\u2013224","journal-title":"Physiol Plant"},{"key":"434_CR5","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1093\/jxb\/erl241","volume":"58","author":"K Burg","year":"2007","unstructured":"Burg K, Helmersson A, Bozhkov P, von Arnold S (2007) Developmental and genetic variation in nuclear microsatellite stability during somatic embryogenesis in pine. J Exp Bot 58:687\u2013698","journal-title":"J Exp Bot"},{"key":"434_CR6","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1023\/A:1010692104861","volume":"64","author":"AC Cassells","year":"2001","unstructured":"Cassells AC, Curry RF (2001) Oxidative stress and physiological, epigenetic and genetic variability in plant tissue culture: implications for micropropagators and genetic engineers. Plant Cell Tissue Organ Cult 64:145\u2013157","journal-title":"Plant Cell Tissue Organ Cult"},{"key":"434_CR7","doi-asserted-by":"crossref","first-page":"4399","DOI":"10.1242\/jcs.113.24.4399","volume":"113","author":"LH Filonova","year":"2000","unstructured":"Filonova LH, Bozhkov PV, Brukhin VB, Daniel G, Zhivotovsky B, von Arnold S (2000a) Two waves of programmed cell death occur during formation and development of somatic embryos in the gymnosperm, Norway spruce. J Cell Sci 113:4399\u20134411","journal-title":"J Cell Sci"},{"key":"434_CR8","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1093\/jexbot\/51.343.249","volume":"51","author":"LH Filonova","year":"2000","unstructured":"Filonova LH, Bozhkov PV, von Arnold S (2000b) Developmental pathway of somatic embryogenesis in Picea abies as revealed by time-lapse tracking. J Exp Bot 51:249\u2013264","journal-title":"J Exp Bot"},{"key":"434_CR9","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1007\/s001220050395","volume":"94","author":"J-L Fourr\u00e9","year":"1997","unstructured":"Fourr\u00e9 J-L, Berger P, Niquet L, Andr\u00e9 P (1997) Somatic embryogenesis and somaclonal variation in Norway spruce: morphogenetic, cytogenetic and molecular approaches. Theor Appl Genet 94:159\u2013169","journal-title":"Theor Appl Genet"},{"key":"434_CR10","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.mrgentox.2006.01.005","volume":"605","author":"T Gichner","year":"2006","unstructured":"Gichner T, Mukherjee A, Velem\u00ednsk\u00fd J (2006) DNA staining with the fluorochromes EtBr, DAPI and YOYO-1 in the comet assay with tobacco plants after treatment with ethyl methanesulphonate, hyperthermia and DNase-I. Mutat Res 605:17\u201321","journal-title":"Mutat Res"},{"key":"434_CR11","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/S0378-4274(96)03847-7","volume":"90","author":"A Hartmann","year":"1997","unstructured":"Hartmann A, Speit G (1997) The contribution of cytotoxicity to DNA-effects in the single cell gel test (comet assay). Toxicol Lett 90:183\u2013188","journal-title":"Toxicol Lett"},{"key":"434_CR12","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1080\/02827580701820043","volume":"23","author":"A Helmersson","year":"2008","unstructured":"Helmersson A, Jansson G, Bozhkov PV, von Arnold S (2008) Genetic variation in microsatellite stability of somatic embryo plants of Picea abies: a case study using six unrelated full-sib families. Scand J Forest Res 23:2\u201311","journal-title":"Scand J Forest Res"},{"key":"434_CR13","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1007\/s11627-008-9158-6","volume":"45","author":"K Klimaszewska","year":"2009","unstructured":"Klimaszewska K, Noceda C, Pelletier G, Label P, Rodriguez R, Lelu-Walter MA (2009) Biological characterization of young and aged embryogenic cultures of Pinus pinaster. Vitro Cell Dev Biol Plant 45:20","journal-title":"Vitro Cell Dev Biol Plant"},{"key":"434_CR14","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/S1383-5742(03)00017-6","volume":"544","author":"RF Lee","year":"2003","unstructured":"Lee RF, Steinert S (2003) Use of the single cell gel electrophoresis\/comet assay for detecting DNA damage in aquatic (marine and freshwater) animals. Mutat Res 544:43\u201364","journal-title":"Mutat Res"},{"key":"434_CR15","first-page":"35","volume-title":"Annales de Recherches Sylvicoles","author":"MA Lelu","year":"1987","unstructured":"Lelu MA (1987) Variations morphologiques et g\u00e9n\u00e9tiques chez Picea abies obtenues apr\u00e8s embryogen\u00e9se somatique. \u00c9tude pr\u00e9liminaire. In: Annales de Recherches Sylvicoles. Association For\u00eat-Cellulose, Paris, pp 35\u201347"},{"key":"434_CR16","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1007\/s00299-008-0668-9","volume":"28","author":"L Marum","year":"2009","unstructured":"Marum L, Rocheta M, Maroco J, Oliveira MM, Miguel C (2009) Analysis of genetic stability at SSR loci during somatic embryogenesis in maritime pine (Pinus pinaster). Plant Cell Rep 28:673\u2013682","journal-title":"Plant Cell Rep"},{"key":"434_CR17","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/S1383-5718(01)00165-6","volume":"495","author":"M Menke","year":"2001","unstructured":"Menke M, Chen I, Angelis KJ, Schubert I (2001) DNA damage and repair in Arabidopsis thaliana as measured by the comet assay after treatment with different classes of genotoxins. Mutat Res 495:87\u201395","journal-title":"Mutat Res"},{"key":"434_CR18","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1007\/BF00270072","volume":"8","author":"LH Mo","year":"1989","unstructured":"Mo LH, von Arnold S, Lagerkranz U (1989) Morphogenic and genetic stability in longterm embryogenic cultures and somatic embryos of Norway spruce (Picea abies L. Karst). Plant Cell Rep 8:375\u2013378","journal-title":"Plant Cell Rep"},{"key":"434_CR19","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/S1383-5718(96)00157-X","volume":"389","author":"MH Navarrete","year":"1997","unstructured":"Navarrete MH, Carrera P, de Miguel M, de la Torre C (1997) A fast comet assay variant for solid tissue cells. The assessment of DNA damage in higher plants. Mutat Res 389:271\u2013277","journal-title":"Mutat Res"},{"key":"434_CR20","doi-asserted-by":"crossref","first-page":"86","DOI":"10.2307\/3577587","volume":"122","author":"PL Olive","year":"1990","unstructured":"Olive PL, Ban\u00e1th JP, Durand RE (1990) Heterogeneity in radiation-induced DNA damage and repair in tumor and normal cells measured using the \u201ccomet\u201d assay. Radiat Res 122:86\u201394","journal-title":"Radiat Res"},{"key":"434_CR21","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1007\/BF00231807","volume":"9","author":"MM Oliveira","year":"1991","unstructured":"Oliveira MM, Pais MSS (1991) Plant regeneration from protoplasts of long-term callus cultures of Actinidia deliciosa var. deliciosa cv. Hayword (Kiwifruit). Plant Cell Rep 9:643\u2013646","journal-title":"Plant Cell Rep"},{"key":"434_CR22","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/0014-4827(88)90265-0","volume":"175","author":"NP Singh","year":"1988","unstructured":"Singh NP, McCoy MT, Tice RR, Schneider EL (1988) A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 175:184\u2013191","journal-title":"Exp Cell Res"},{"key":"434_CR23","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1016\/j.envpol.2009.10.005","volume":"158","author":"H Tai","year":"2010","unstructured":"Tai H, Percy K, Karnosky D (2010) DNA damage in Populus tremuloides clones exposed to elevated O3. Environ Pollut 158:969\u2013976","journal-title":"Environ Pollut"},{"key":"434_CR24","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.mrrev.2008.11.001","volume":"681","author":"M Valverde","year":"2009","unstructured":"Valverde M, Rojas E (2009) Environmental and occupational biomonitoring using the comet assay. Mutat Res 681:93\u2013109","journal-title":"Mutat Res"},{"key":"434_CR25","doi-asserted-by":"crossref","first-page":"3354","DOI":"10.1016\/j.envpol.2009.09.012","volume":"157","author":"M Villarini","year":"2009","unstructured":"Villarini M, Fatigoni C, Dominici L, Maestri S, Ederli L, Pasqualini S, Monarca S, Moretti M (2009) Assessing the genotoxicity of urban air pollutants using two in situ plant bioassays. Environ Pollut 157:3354\u20133356","journal-title":"Environ Pollut"},{"key":"434_CR26","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1023\/A:1024614209524","volume":"75","author":"PRP Aderkas von","year":"2003","unstructured":"von Aderkas PRP, Hristoforoglu K, Ma Y (2003) Embryogenesis and genetic stability in long term megagametophyte-derived cultures of larch. Plant Cell Tissue Organ Cult 75:27\u201334","journal-title":"Plant Cell Tissue Organ Cult"},{"key":"434_CR27","first-page":"929","volume-title":"Biostatistical analysis","author":"JH Zar","year":"1998","unstructured":"Zar JH (1998) Biostatistical analysis, 4th edn. Prentice Hall, Upper Saddle River, p 929","edition":"4"}],"container-title":["Tree Genetics &amp; Genomes"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s11295-011-0434-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s11295-011-0434-y\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s11295-011-0434-y","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,7]],"date-time":"2021-12-07T09:30:21Z","timestamp":1638869421000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s11295-011-0434-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,9,25]]},"references-count":27,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2012,4]]}},"alternative-id":["434"],"URL":"https:\/\/doi.org\/10.1007\/s11295-011-0434-y","relation":{},"ISSN":["1614-2942","1614-2950"],"issn-type":[{"value":"1614-2942","type":"print"},{"value":"1614-2950","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,9,25]]}}}