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The aim was the ascertainment of the Zn and Cu concentrations in piglets\u2019 liver and kidney and their impact in the reduced susceptibility to Zn, Cu, and antibiotics in enterococci, used as microbiota biomarker. Zn and Cu were determined in the livers and kidneys of 43 piglets slaughtered in Portugal, by flame atomic absorption spectrometry. Enterococci were isolated from feces for determining the identification of species (<jats:italic>E. faecalis<\/jats:italic>, <jats:italic>E. faecium<\/jats:italic>, and <jats:italic>Enterococcus<\/jats:italic> spp.); susceptibility to vancomycin, ciprofloxacin, linezolid, tigecycline, ampicillin, imipenem, and metals; and Cu tolerance genes. In piglets with Zn and Cu high or toxic levels, enterococci had reduced susceptibility to ions, reinforced by the presence of Cu tolerance genes and by resistance to antibiotics. The study relevance is to show the relationship between these metals\u2019 levels and decreased susceptibility to Cu, Zn, and antibiotics by enterococci. From the results, it could be supposed that the piglets were being fed with high doses of Zn and Cu which could select more resistant bacteria to both antibiotics and metals that could spread to environment and humans.<\/jats:p>","DOI":"10.1007\/s11356-024-32495-8","type":"journal-article","created":{"date-parts":[[2024,2,21]],"date-time":"2024-02-21T09:02:22Z","timestamp":1708506142000},"page":"20941-20952","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Assessment of Zn and Cu in piglets\u2019 liver and kidney: impact in fecal Enterococcus spp.?"],"prefix":"10.1007","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0543-0088","authenticated-orcid":false,"given":"Maria M.","family":"Donato","sequence":"first","affiliation":[]},{"given":"Gabriela","family":"Assis","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8902-0213","authenticated-orcid":false,"given":"Olga","family":"Cardoso","sequence":"additional","affiliation":[]},{"given":"B\u00e1rbara","family":"Oliveiros","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3292-5473","authenticated-orcid":false,"given":"Andreia","family":"Freitas","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6043-819X","authenticated-orcid":false,"given":"Fernando","family":"Ramos","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2024,2,21]]},"reference":[{"key":"32495_CR1","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1016\/j.vetmic.2004.01.013","volume":"100","author":"FM Aarestrup","year":"2004","unstructured":"Aarestrup FM, Hasman H (2004) Susceptibility of different bacterial species isolated from food animals to copper sulphate, zinc chloride and antimicrobial substances used for disinfection. 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