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In this study, we aimed to understand whether effluents of three different WWTPs may have ecological effects in riverine recipient ecosystems. To achieve this, we assessed benthic phytobenthos and macroinvertebrate communities at three different locations relative to the effluent discharge: immediately upstream, immediately downstream and 500-m downstream the effluent discharge. Two approaches were employed: the ecological status classification as defined in the Water Framework Directive (WFD) based on biological indicators; constrained multivariate analysis to disentangle the environmental drivers (physicochemical variables and contaminants, namely metals, polycyclic aromatic hydrocarbons, pharmaceuticals, and personal care products) of ecological changes across the study sites. The results showed inconsistencies between the WFD approach and the multivariate approach, as well as between the responses of macroinvertebrates and diatoms. The WWTP effluents impacted benthic communities in a single case: macroinvertebrates were negatively affected by one of the WWTP effluents, likely by the transported pharmaceuticals (other stressors are essentially homogeneous among sites). Given the findings and the scarcity of consistent evidence on ecological impacts that WWTP effluents may have in recipient ecosystems, further research is needed towards more sustainable regulation and linked environmental protection measures.<\/jats:p>","DOI":"10.1007\/s11356-024-33462-z","type":"journal-article","created":{"date-parts":[[2024,5,6]],"date-time":"2024-05-06T06:02:28Z","timestamp":1714975348000},"page":"34492-34506","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Potential effects of the discharge of wastewater treatment plant (WWTP) effluents in benthic communities: evidence from three distinct WWTP systems"],"prefix":"10.1007","volume":"31","author":[{"given":"Carlos","family":"Silva","sequence":"first","affiliation":[]},{"given":"Joana Isabel","family":"Santos","sequence":"additional","affiliation":[]},{"given":"T\u00e2nia","family":"Vidal","sequence":"additional","affiliation":[]},{"given":"Susana","family":"Silva","sequence":"additional","affiliation":[]},{"given":"Salom\u00e9 Fernandes Pinheiro","family":"Almeida","sequence":"additional","affiliation":[]},{"given":"Fernando Jos\u00e9 Mendes","family":"Gon\u00e7alves","sequence":"additional","affiliation":[]},{"given":"Nelson","family":"Abrantes","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7573-6184","authenticated-orcid":false,"given":"Joana Lu\u00edsa","family":"Pereira","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2024,5,6]]},"reference":[{"key":"33462_CR1","doi-asserted-by":"publisher","first-page":"51","DOI":"10.23818\/limn.04.06","volume":"4","author":"J Alba-Tercedor","year":"1988","unstructured":"Alba-Tercedor J (1988) Un m\u00e9todo r\u00e1pido y simple para evaluar la calidad biol\u00f3gica de las aguas corrientes basado en el de Hellawell 1978. 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