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It has been proposed that the \u201c<jats:italic>trans<\/jats:italic>\u201010 shifted\u201d biohydrogenation pathway of \u03b1\u2010linolenic acid generates two intermediates, the <jats:italic>trans<\/jats:italic>\u201010,<jats:italic>cis<\/jats:italic>\u201015 18:2 and <jats:italic>trans<\/jats:italic>\u201010,<jats:italic>cis<\/jats:italic>\u201012,<jats:italic>cis<\/jats:italic>\u201015 18:3, although none of these have been found in the rumen. We analyzed digestive contents and meat samples from two trials, where animals were fed: a compound feed diet supplemented with 8 % oil blend containing linseed oil (samples A); and a forage based diet supplemented with 6 % linseed oil (samples B). The use of the new SLB\u2010IL111 chromatographic column allowed the detection of two different 18:2 isomers in each sample trial, which could not be resolved when the CP\u2010Sil 88 column is used. The two 18:2 isomers were characterized by mass spectrometry using 4,4\u2010dimethyloxazoline derivatives. However, because they were subject to higher temperatures and present different chromatographic properties compared with the fatty acid methyl esters, we also used the \u201ccovalent adduct chemical ionization\u201d technique to confirm the identity of both 18:2 isomers. We detected and identified the 10,15\u201018:2 in samples A and the 11,15\u201018:2 in samples B. The geometry of both isomers was tentatively assigned as <jats:italic>trans,cis<\/jats:italic> taking in account their elution order and biologic plausibility. As far as we know, this is the first time that the <jats:italic>trans<\/jats:italic>\u201010,<jats:italic>cis<\/jats:italic>\u201015 18:2 has been found in ruminant digestive contents and meat samples associated with the \u201c<jats:italic>trans<\/jats:italic>\u201010 shifted\u201d biohydrogenation pathway of \u03b1\u2010linolenic acid.<\/jats:p>","DOI":"10.1007\/s11745-014-3897-4","type":"journal-article","created":{"date-parts":[[2014,3,27]],"date-time":"2014-03-27T02:52:26Z","timestamp":1395888746000},"page":"527-541","source":"Crossref","is-referenced-by-count":75,"title":["The <i>trans<\/i>\u201010,<i>cis<\/i>\u201015 18:2: a Missing Intermediate of <i>trans<\/i>\u201010 Shifted Rumen Biohydrogenation Pathway?"],"prefix":"10.1002","volume":"49","author":[{"given":"Susana P.","family":"Alves","sequence":"first","affiliation":[{"name":"CIISA, Faculdade de Medicina Veterin\u00e1ria (FMV) Universidade de Lisboa (ULisboa) Av. da Universidade T\u00e9cnica Lisbon 1300\u2010477 Portugal"}]},{"given":"Rui J. 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