{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T12:52:58Z","timestamp":1761310378628,"version":"build-2065373602"},"reference-count":19,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,1,1]],"date-time":"2006-01-01T00:00:00Z","timestamp":1136073600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Americ Oil Chem Soc"],"published-print":{"date-parts":[[2006,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A biphasic oil\u2010aqueous system for FAME production by enzymatic catalysis was studied. The transesterification of sunflower oil with methanol was catalyzed by free or immobilized lipases from <jats:italic>Rhizomucor miehei<\/jats:italic> (Palatase 20 000 L) and <jats:italic>Humicola insolens<\/jats:italic> (Lipozyme TL 100 L). The effects of protein amount, temperature, pH, and molar ratio of methanol to sunflower oil on the enzymatic reaction using free lipase were evaluated; the best results were obtained with <jats:italic>H. insolens<\/jats:italic>, at pH 5, 40\u00b0C, and 36.8 mg of protein. By using this lipase immobilized in Hypol<jats:sup>\u00ae<\/jats:sup> 2002 (64. 8 mg of protein) at a 6\u22361 methanol\/oil molar ratio and a 2\u22361 volumetric oil\/water phase ratio, an ester content of 96.1% and a conversion of 91.2% were achieved. The immobilized lipase could be reused, although a 30% reduction in conversion efficiency was observed after four uses.<\/jats:p>","DOI":"10.1007\/s11746-006-1170-6","type":"journal-article","created":{"date-parts":[[2006,11,28]],"date-time":"2006-11-28T12:57:02Z","timestamp":1164718622000},"page":"21-25","source":"Crossref","is-referenced-by-count":42,"title":["Enzymatic transesterification of sunflower oil in an aqueous\u2010oil biphasic system"],"prefix":"10.1002","volume":"83","author":[{"given":"A. C.","family":"Oliveira","sequence":"first","affiliation":[{"name":"Departamento de Energias Renov\u00e1veis Instituto Nacional de Engenharia, Tecnologia e Inova\u00e7\u00e3o Estrada do Pa\u00e7o do Lumiar Lisbon 1649\u2010038 Portugal"}]},{"given":"M. F.","family":"Rosa","sequence":"additional","affiliation":[{"name":"Departamento de Energias Renov\u00e1veis Instituto Nacional de Engenharia, Tecnologia e Inova\u00e7\u00e3o Estrada do Pa\u00e7o do Lumiar Lisbon 1649\u2010038 Portugal"}]}],"member":"311","published-online":{"date-parts":[[2006,1]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0141-0229(01)00453-7"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02523383"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0141-0229(03)00090-5"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-8524(01)00203-6"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/10242420410001661222"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1381-1177(02)00022-X"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1389-1723(00)87091-7"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0032-9592(02)00241-8"},{"key":"e_1_2_1_10_1","first-page":"405","article-title":"Lipase Catalyzed Methanolysis of Vegetable Oils in Aqueous Medium by Cryptococcus spp. S\u20102","volume":"37","author":"Kamini N.R.","year":"2001","journal-title":"Ibid."},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0141-0229(99)00185-4"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_1_13_1","unstructured":"NP 903 Gorduras e \u00d3leos Comest\u00edveis. 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