{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T10:23:51Z","timestamp":1762251831021,"version":"build-2065373602"},"reference-count":31,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2012,2,8]],"date-time":"2012-02-08T00:00:00Z","timestamp":1328659200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["CTQ2010\u201015131"],"award-info":[{"award-number":["CTQ2010\u201015131"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002809","name":"Generalitat de Catalunya","doi-asserted-by":"publisher","award":["2009\u2010SGR\u2010281"],"award-info":[{"award-number":["2009\u2010SGR\u2010281"]}],"id":[{"id":"10.13039\/501100002809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Americ Oil Chem Soc"],"published-print":{"date-parts":[[2012,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Response surface methodology was used to model and optimize the acidolysis of virgin olive oil with caprylic (C8:0) or capric (C10:0) acids, aimed at the production of low caloric triacylglycerols (TAG) of MLM type, in solvent free media, catalyzed by the heterologous <jats:italic>Rhizopus oryzae<\/jats:italic> lipase (r\u2010ROL) immobilized in Eupergit<jats:sup>\u00ae<\/jats:sup> C. This lipase was produced in the methylotrophic yeast <jats:italic>Pichia pastoris<\/jats:italic> Mut<jats:sup>s<\/jats:sup> phenotype (experiments with C10:0) or a Mut<jats:sup>+<\/jats:sup> phenotype (experiments with C8:0), under different operational conditions. The r\u2010ROL used in experiments with C10:0 presented a hydrolytic activity about 5 times of that presented by r\u2010ROL used in acidolysis with C8:0. The experiments were carried out following a central composite rotatable design, as a function of the molar ratio (MR) medium chain fatty acid\/TAG (1.6\u20134.4) and temperature (25\u201355 \u00b0C). Convex surfaces described by second order polynomials as a function of MR and temperature were well fitted to fatty acid incorporation values. After 24\u2010h reaction, the predicted maximum incorporation of caprylic (15.5 mol%) or capric (33.3 mol%) acids in olive oil occurs at 37 and 35 \u00b0C, respectively, and at C8:0\/TAG of 2.8:1 or C10:0\/TAG of 3:1. These predicted optima were experimentally validated. Fermentation conditions used in r\u2010ROL production highly affected hydrolytic activity and to a lesser extent interesterification activity.<\/jats:p>","DOI":"10.1007\/s11746-012-2027-9","type":"journal-article","created":{"date-parts":[[2012,2,6]],"date-time":"2012-02-06T23:51:13Z","timestamp":1328572273000},"page":"1287-1295","source":"Crossref","is-referenced-by-count":27,"title":["Optimized Production of MLM Triacylglycerols Catalyzed by Immobilized Heterologous <i>Rhizopus oryzae<\/i> Lipase"],"prefix":"10.1002","volume":"89","author":[{"given":"P. A.","family":"Nunes","sequence":"first","affiliation":[{"name":"Instituto Superior de Engenharia Universidade do Algarve, Campus da Penha Faro 8005\u2010139 Portugal"},{"name":"Instituto Superior de Agronomia, CEER\u2010Biosystems Engineering Technical University of Lisbon Tapada da Ajuda Lisboa 1349\u2010017 Portugal"}]},{"given":"P.","family":"Pires\u2010Cabral","sequence":"additional","affiliation":[{"name":"Instituto Superior de Engenharia Universidade do Algarve, Campus da Penha Faro 8005\u2010139 Portugal"},{"name":"Instituto Superior de Agronomia, CEER\u2010Biosystems Engineering Technical University of Lisbon Tapada da Ajuda Lisboa 1349\u2010017 Portugal"}]},{"given":"M.","family":"Guill\u00e9n","sequence":"additional","affiliation":[{"name":"Departament d'Enginyeria Qu\u00edmica, EE Universitat Aut\u00f2noma de Barcelona 08193 Bellaterra Barcelona Spain"}]},{"given":"F.","family":"Valero","sequence":"additional","affiliation":[{"name":"Departament d'Enginyeria Qu\u00edmica, EE Universitat Aut\u00f2noma de Barcelona 08193 Bellaterra Barcelona Spain"}]},{"given":"S.","family":"Ferreira\u2010Dias","sequence":"additional","affiliation":[{"name":"Instituto Superior de Agronomia, CEER\u2010Biosystems Engineering Technical University of Lisbon Tapada da Ajuda Lisboa 1349\u2010017 Portugal"}]}],"member":"311","published-online":{"date-parts":[[2012,2,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1438-9312(200004)102:4<287::AID-EJLT287>3.0.CO;2-Q"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf00038a036"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1541-4337.2002.tb00010.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1381-1177(00)00120-X"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2621.1996.tb14743.x"},{"key":"e_1_2_7_7_1","first-page":"885","article-title":"Enzymatic acidolysis in hexane to produce n\u20103 or n\u20106 FA\u2010enriched structured lipids from coconut oil: optimization of reactions by response surface methodology","volume":"79","author":"Reena R","year":"2001","journal-title":"J Am Oil Chem Soc"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfoodeng.2007.05.016"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0963-9969(00)00158-7"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.procbio.2009.07.005"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf0103020"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf0509761"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11746-005-1038-9"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.3297"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2010.04.096"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11746-010-1702-y"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2011.01.071"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bej.2011.02.008"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2007.01.010"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.enzmictec.2010.01.005"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2004.12.010"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2003.10.029"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_7_24_1","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/B978-0-08-091831-0.50012-6","volume-title":"Statistical Methods in Food and Consumer Research, Food Science and Technology. A Series of Monographs","author":"Gacula MC","year":"1984"},{"key":"e_1_2_7_25_1","first-page":"258","volume-title":"Experimental design in biotechnology, statistics: textbooks and monographs","author":"Haaland PD","year":"1989"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11746-999-0085-4"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/jctb.810"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf000372r"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0141-0229(95)00239-1"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0734-9750(01)00086-6"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(91)90594-2"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-4133(199910)101:10<364::AID-LIPI364>3.0.CO;2-0"}],"container-title":["Journal of the American Oil Chemists' Society"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aocs.onlinelibrary.wiley.com\/doi\/pdf\/10.1007\/s11746-012-2027-9","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T18:32:45Z","timestamp":1759948365000},"score":1,"resource":{"primary":{"URL":"https:\/\/aocs.onlinelibrary.wiley.com\/doi\/10.1007\/s11746-012-2027-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,2,8]]},"references-count":31,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2012,7]]}},"alternative-id":["10.1007\/s11746-012-2027-9"],"URL":"https:\/\/doi.org\/10.1007\/s11746-012-2027-9","archive":["Portico"],"relation":{},"ISSN":["0003-021X","1558-9331"],"issn-type":[{"type":"print","value":"0003-021X"},{"type":"electronic","value":"1558-9331"}],"subject":[],"published":{"date-parts":[[2012,2,8]]}}}