{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T22:25:01Z","timestamp":1761863101127,"version":"build-2065373602"},"reference-count":48,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2012,2,11]],"date-time":"2012-02-11T00:00:00Z","timestamp":1328918400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Americ Oil Chem Soc"],"published-print":{"date-parts":[[2012,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In this study, a new HPLC method was developed and validated for the determination of daidzin, genistin, glycitin, and their respective aglycones daidzein, genistein and glycitein in extracts from different stages of soy processing. The chromatographic separation was achieved using a Bonus RP 100 \u00d7 3.0 mm (3.5\u2010\u03bcm particle size) column maintained at 35 \u00b0C. The mobile phase consisted of a gradient of water and acetonitrile both containing 0.1% formic acid. Analyses were conducted at a flow rate of 1.0 mL min<jats:sup>\u22121<\/jats:sup> and the detector was set at 254 nm. Validation parameters indicated that the method has high selectivity and is linear over a range of 1.5\u2013150 \u00b5g mL<jats:sup>\u22121<\/jats:sup> for all compounds. The range of recovery was 95\u2013102% with a RSD% &lt;5% for intra\u2010day and inter\u2010day precision. The robustness study indicated that the flow rate was the only critical factor. The new HPLC\u2013DAD method was successfully applied to real samples and excellent separation was achieved without the need of any sample pretreatment.<\/jats:p>","DOI":"10.1007\/s11746-012-2030-1","type":"journal-article","created":{"date-parts":[[2012,2,9]],"date-time":"2012-02-09T21:14:22Z","timestamp":1328822062000},"page":"1211-1222","source":"Crossref","is-referenced-by-count":12,"title":["Development and Validation of an HPLC\u2013DAD Method for Analysis of the Six Major Isoflavones in Extracts from Soybean Processing"],"prefix":"10.1002","volume":"89","author":[{"given":"J. C.","family":"Gasparetto","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Center of Biopharmacy Universidade Federal do Paran\u00e1 632 Loth\u00e1rio Meissner Avenue Curitiba PR 80210\u2010170 Brazil"}]},{"given":"F. S. F.","family":"Smolarek","sequence":"additional","affiliation":[{"name":"Department of Research Imcopa S\/A Arauc\u00e1ria Brazil"}]},{"given":"T. M. G.","family":"de Francisco","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Center of Biopharmacy Universidade Federal do Paran\u00e1 632 Loth\u00e1rio Meissner Avenue Curitiba PR 80210\u2010170 Brazil"}]},{"given":"L. C.","family":"Miranda","sequence":"additional","affiliation":[{"name":"Department of Research Imcopa S\/A Arauc\u00e1ria Brazil"}]},{"given":"R.","family":"Pontarolo","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Center of Biopharmacy Universidade Federal do Paran\u00e1 632 Loth\u00e1rio Meissner Avenue Curitiba PR 80210\u2010170 Brazil"}]},{"given":"P. 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