{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T00:03:46Z","timestamp":1774915426826,"version":"3.50.1"},"reference-count":39,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2011,3,24]],"date-time":"2011-03-24T00:00:00Z","timestamp":1300924800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cardiovasc Toxicol"],"published-print":{"date-parts":[[2011,6]]},"DOI":"10.1007\/s12012-011-9112-4","type":"journal-article","created":{"date-parts":[[2011,3,23]],"date-time":"2011-03-23T14:36:51Z","timestamp":1300891011000},"page":"180-190","source":"Crossref","is-referenced-by-count":54,"title":["Metabolic Remodeling During H9c2 Myoblast Differentiation: Relevance for In Vitro Toxicity Studies"],"prefix":"10.1007","volume":"11","author":[{"given":"Sandro L.","family":"Pereira","sequence":"first","affiliation":[]},{"given":"Jo\u00e3o","family":"Ramalho-Santos","sequence":"additional","affiliation":[]},{"given":"Ana F.","family":"Branco","sequence":"additional","affiliation":[]},{"given":"Vilma A.","family":"Sard\u00e3o","sequence":"additional","affiliation":[]},{"given":"Paulo J.","family":"Oliveira","sequence":"additional","affiliation":[]},{"given":"Rui A.","family":"Carvalho","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2011,3,24]]},"reference":[{"key":"9112_CR1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0012-1606(03)00112-X","volume":"258","author":"T Brand","year":"2003","unstructured":"Brand, T. (2003). Heart development: Molecular insights into cardiac specification and early morphogenesis. Developmental Biology, 258, 1\u201319.","journal-title":"Developmental Biology"},{"key":"9112_CR2","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1023\/A:1015332726303","volume":"7","author":"JJ Lehman","year":"2002","unstructured":"Lehman, J. J., & Kelly, D. P. (2002). Gene regulatory mechanisms governing energy metabolism during cardiac hypertrophic growth. Heart Failure Reviews, 7, 175\u2013185.","journal-title":"Heart Failure Reviews"},{"key":"9112_CR3","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1046\/j.1440-1681.2002.03655.x","volume":"29","author":"JJ Lehman","year":"2002","unstructured":"Lehman, J. J., & Kelly, D. P. (2002). Transcriptional activation of energy metabolic switches in the developing and hypertrophied heart. Clinical and Experimental Pharmacology and Physiology, 29, 339\u2013345.","journal-title":"Clinical and Experimental Pharmacology and Physiology"},{"key":"9112_CR4","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1023\/A:1006860104840","volume":"188","author":"AO Makinde","year":"1998","unstructured":"Makinde, A. O., Kantor, P. F., & Lopaschuk, G. D. (1998). Maturation of fatty acid and carbohydrate metabolism in the newborn heart. Molecular and Cellular Biochemistry, 188, 49\u201356.","journal-title":"Molecular and Cellular Biochemistry"},{"key":"9112_CR5","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1097\/FJC.0b013e3181e74a14","volume":"56","author":"GD Lopaschuk","year":"2010","unstructured":"Lopaschuk, G. D., & Jaswal, J. S. (2010). Energy metabolic phenotype of the cardiomyocyte during development, differentiation, and postnatal maturation. Journal of Cardiovascular Pharmacology, 56, 130\u2013140.","journal-title":"Journal of Cardiovascular Pharmacology"},{"key":"9112_CR6","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1203\/00006450-198105000-00003","volume":"15","author":"DJ Fisher","year":"1981","unstructured":"Fisher, D. J., Heymann, M. A., & Rudolph, A. M. (1981). Myocardial consumption of oxygen and carbohydrates in newborn sheep. Pediatric Research, 15, 843\u2013846.","journal-title":"Pediatric Research"},{"key":"9112_CR7","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1203\/00006450-198711000-00016","volume":"22","author":"JC Werner","year":"1987","unstructured":"Werner, J. C., & Sicard, R. E. (1987). Lactate metabolism of isolated, perfused fetal, and newborn pig hearts. Pediatric Research, 22, 552\u2013556.","journal-title":"Pediatric Research"},{"key":"9112_CR8","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1152\/physrev.1992.72.2.507","volume":"72","author":"J Girard","year":"1992","unstructured":"Girard, J., Ferre, P., Pegorier, J. P., & Duee, P. H. (1992). Adaptations of glucose and fatty acid metabolism during perinatal period and suckling-weaning transition. Physiological Reviews, 72, 507\u2013562.","journal-title":"Physiological Reviews"},{"key":"9112_CR9","first-page":"H1698","volume":"261","author":"GD Lopaschuk","year":"1991","unstructured":"Lopaschuk, G. D., Spafford, M. A., & Marsh, D. R. (1991). Glycolysis is predominant source of myocardial ATP production immediately after birth. American Journal of Physiology, 261, H1698\u2013H1705.","journal-title":"American Journal of Physiology"},{"key":"9112_CR10","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1051\/rnd:19860413","volume":"26","author":"P Ferre","year":"1986","unstructured":"Ferre, P., Decaux, J. F., Issad, T., & Girard, J. (1986). Changes in energy metabolism during the suckling and weaning period in the newborn. Reproduction, Nutrition, Development, 26, 619\u2013631.","journal-title":"Reproduction, Nutrition, Development"},{"key":"9112_CR11","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/0146-2806(94)90008-6","volume":"19","author":"H Taegtmeyer","year":"1994","unstructured":"Taegtmeyer, H. (1994). Energy metabolism of the heart: from basic concepts to clinical applications. Current Problems in Cardiology, 19, 59\u2013113.","journal-title":"Current Problems in Cardiology"},{"key":"9112_CR12","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/0014-4827(76)90447-X","volume":"98","author":"BW Kimes","year":"1976","unstructured":"Kimes, B. W., & Brandt, B. L. (1976). Properties of a clonal muscle cell line from rat heart. Experimental Cell Research, 98, 367\u2013381.","journal-title":"Experimental Cell Research"},{"key":"9112_CR13","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1002\/jcp.10420","volume":"198","author":"M Pagano","year":"2004","unstructured":"Pagano, M., Naviglio, S., Spina, A., Chiosi, E., Castoria, G., Romano, M., et al. (2004). Differentiation of H9c2 cardiomyoblasts: The role of adenylate cyclase system. Journal of Cellular Physiology, 198, 408\u2013416.","journal-title":"Journal of Cellular Physiology"},{"key":"9112_CR14","doi-asserted-by":"crossref","first-page":"H1822","DOI":"10.1152\/ajpheart.00396.2008","volume":"296","author":"R Saeedi","year":"2009","unstructured":"Saeedi, R., Saran, V. V., Wu, S. S., Kume, E. S., Paulson, K., Chan, A. P., et al. (2009). AMP-activated protein kinase influences metabolic remodeling in H9c2 cells hypertrophied by arginine vasopressin. American Journal of Physiology Heart Circulatory Physiology, 296, H1822\u2013H1832.","journal-title":"American Journal of Physiology Heart Circulatory Physiology"},{"key":"9112_CR15","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/1471-2121-8-11","volume":"8","author":"VA Sardao","year":"2007","unstructured":"Sardao, V. A., Oliveira, P. J., Holy, J., Oliveira, C. R., & Wallace, K. B. (2007). Vital imaging of H9c2 myoblasts exposed to tert-butylhydroperoxide\u2014characterization of morphological features of cell death. BMC Cell Biology, 8, 11.","journal-title":"BMC Cell Biology"},{"key":"9112_CR16","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1007\/s00280-009-0932-x","volume":"64","author":"VA Sardao","year":"2009","unstructured":"Sardao, V. A., Oliveira, P. J., Holy, J., Oliveira, C. R., & Wallace, K. B. (2009). Doxorubicin-induced mitochondrial dysfunction is secondary to nuclear p53 activation in H9c2 cardiomyoblasts. Cancer Chemotherapy and Pharmacology, 64, 811\u2013827.","journal-title":"Cancer Chemotherapy and Pharmacology"},{"key":"9112_CR17","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1007\/s10565-008-9070-1","volume":"25","author":"VA Sardao","year":"2009","unstructured":"Sardao, V. A., Oliveira, P. J., Holy, J., Oliveira, C. R., & Wallace, K. B. (2009). Morphological alterations induced by doxorubicin on H9c2 myoblasts: Nuclear, mitochondrial, and cytoskeletal targets. Cell Biology and Toxicology, 25, 227\u2013243.","journal-title":"Cell Biology and Toxicology"},{"key":"9112_CR18","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1186\/1471-2121-11-29","volume":"11","author":"A Cselenyak","year":"2010","unstructured":"Cselenyak, A., Pankotai, E., Horvath, E. M., Kiss, L., & Lacza, Z. (2010). Mesenchymal stem cells rescue cardiomyoblasts from cell death in an in vitro ischemia model via direct cell-to-cell connections. BMC Cell Biology, 11, 29.","journal-title":"BMC Cell Biology"},{"key":"9112_CR19","doi-asserted-by":"crossref","first-page":"H1466","DOI":"10.1152\/ajpheart.00795.2008","volume":"296","author":"P Mukhopadhyay","year":"2009","unstructured":"Mukhopadhyay, P., Rajesh, M., Batkai, S., Kashiwaya, Y., Hasko, G., Liaudet, L., et al. (2009). Role of superoxide, nitric oxide, and peroxynitrite in doxorubicin-induced cell death in vivo and in vitro. American Journal of Physiology Heart Circulatory Physiology, 296, H1466\u2013H1483.","journal-title":"American Journal of Physiology Heart Circulatory Physiology"},{"key":"9112_CR20","doi-asserted-by":"crossref","first-page":"29063","DOI":"10.1074\/jbc.274.41.29063","volume":"274","author":"C Menard","year":"1999","unstructured":"Menard, C., Pupier, S., Mornet, D., Kitzmann, M., Nargeot, J., & Lory, P. (1999). Modulation of L-type calcium channel expression during retinoic acid-induced differentiation of H9C2 cardiac cells. J Biol Chem, 274, 29063\u201329070.","journal-title":"J Biol Chem"},{"key":"9112_CR21","first-page":"2652","volume":"122","author":"Z Liu","year":"2009","unstructured":"Liu, Z., Song, X. D., Xin, Y., Wang, X. J., Yu, H., Bai, Y. Y., et al. (2009). Protective effect of chrysoeriol against doxorubicin-induced cardiotoxicity in vitro. Chinese Medicine Journal, 122, 2652\u20132656. (Engl).","journal-title":"Chinese Medicine Journal"},{"key":"9112_CR22","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.ab.2008.10.002","volume":"385","author":"AP Rolo","year":"2009","unstructured":"Rolo, A. P., Palmeira, C. M., & Cortopassi, G. A. (2009). Biosensor plates detect mitochondrial physiological regulators and mutations in vivo. Analytical Biochemistry, 385, 176\u2013178.","journal-title":"Analytical Biochemistry"},{"key":"9112_CR23","doi-asserted-by":"crossref","first-page":"15136","DOI":"10.1073\/pnas.0807581105","volume":"105","author":"WS Choi","year":"2008","unstructured":"Choi, W. S., Kruse, S. E., Palmiter, R. D., & Xia, Z. (2008). Mitochondrial complex I inhibition is not required for dopaminergic neuron death induced by rotenone, MPP\u00a0+\u00a0, or paraquat. Proceedings of National Academy of Science USA, 105, 15136\u201315141.","journal-title":"Proceedings of National Academy of Science USA"},{"key":"9112_CR24","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/0012-1606(90)90054-M","volume":"140","author":"RE Swiderski","year":"1990","unstructured":"Swiderski, R. E., & Solursh, M. (1990). Precocious appearance of cardiac troponin T pre-mRNAs during early avian embryonic skeletal muscle development in ovo. Developmental Biology, 140, 73\u201382.","journal-title":"Developmental Biology"},{"key":"9112_CR25","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1016\/j.yjmcc.2010.01.015","volume":"48","author":"KM Ng","year":"2010","unstructured":"Ng, K. M., Lee, Y. K., Chan, Y. C., Lai, W. H., Fung, M. L., Li, R. A., et al. (2010). Exogenous expression of HIF-1 alpha promotes cardiac differentiation of embryonic stem cells. Journal of Molecular and Cellular Cardiology, 48, 1129\u20131137.","journal-title":"Journal of Molecular and Cellular Cardiology"},{"key":"9112_CR26","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1111\/j.1432-0436.2007.00174.x","volume":"75","author":"E Bettiol","year":"2007","unstructured":"Bettiol, E., Sartiani, L., Chicha, L., Krause, K. H., Cerbai, E., & Jaconi, M. E. (2007). Fetal bovine serum enables cardiac differentiation of human embryonic stem cells. Differentiation, 75, 669\u2013681.","journal-title":"Differentiation"},{"key":"9112_CR27","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1002\/nbm.1940020516","volume":"2","author":"B Kunnecke","year":"1989","unstructured":"Kunnecke, B., & Cerdan, S. (1989). Multilabeled 13C substrates as probes in in vivo 13C and 1H NMR spectroscopy. NMR in Biomedicine, 2, 274\u2013277.","journal-title":"NMR in Biomedicine"},{"key":"9112_CR28","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1007\/s004290100206","volume":"204","author":"M Leu","year":"2001","unstructured":"Leu, M., Ehler, E., & Perriard, J. C. (2001). Characterisation of postnatal growth of the murine heart. Anatomy and Embryology, 204, 217\u2013224. (Berl).","journal-title":"Anatomy and Embryology"},{"key":"9112_CR29","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1126\/science.1160809","volume":"324","author":"MG Vander Heiden","year":"2009","unstructured":"Vander Heiden, M. G., Cantley, L. C., & Thompson, C. B. (2009). Understanding the Warburg effect: The metabolic requirements of cell proliferation. Science, 324, 1029\u20131033.","journal-title":"Science"},{"key":"9112_CR30","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1007\/s12015-009-9058-0","volume":"5","author":"JM Facucho-Oliveira","year":"2009","unstructured":"Facucho-Oliveira, J. M., & St John, J. C. (2009). The relationship between pluripotency and mitochondrial DNA proliferation during early embryo development and embryonic stem cell differentiation. Stem Cell Reviews, 5, 140\u2013158.","journal-title":"Stem Cell Reviews"},{"key":"9112_CR31","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1093\/humupd\/dmp016","volume":"15","author":"J Ramalho-Santos","year":"2009","unstructured":"Ramalho-Santos, J., Varum, S., Amaral, S., Mota, P. C., Sousa, A. P., & Amaral, A. (2009). Mitochondrial functionality in reproduction: From gonads and gametes to embryos and embryonic stem cells. Human Reproduction Update, 15, 553\u2013572.","journal-title":"Human Reproduction Update"},{"key":"9112_CR32","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.1016\/j.bbabio.2010.03.025","volume":"1797","author":"SP Mathupala","year":"2010","unstructured":"Mathupala, S. P., Ko, Y. H., & Pedersen, P. L. (2010). The pivotal roles of mitochondria in cancer: Warburg and beyond and encouraging prospects for effective therapies. Biochimica et Biophysica Acta, 1797, 1225\u20131230.","journal-title":"Biochimica et Biophysica Acta"},{"key":"9112_CR33","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1038\/sj.jcbfm.9600400","volume":"27","author":"E Olstad","year":"2007","unstructured":"Olstad, E., Qu, H., & Sonnewald, U. (2007). Glutamate is preferred over glutamine for intermediary metabolism in cultured cerebellar neurons. Journal of Cerebral Blood Flow and Metabolism, 27, 811\u2013820.","journal-title":"Journal of Cerebral Blood Flow and Metabolism"},{"key":"9112_CR34","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/j.neuint.2003.10.010","volume":"45","author":"U Sonnewald","year":"2004","unstructured":"Sonnewald, U., Schousboe, A., Qu, H., & Waagepetersen, H. S. (2004). Intracellular metabolic compartmentation assessed by 13C magnetic resonance spectroscopy. Neurochemistry International, 45, 305\u2013310.","journal-title":"Neurochemistry International"},{"key":"9112_CR35","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1002\/nbm.860","volume":"17","author":"N Anousis","year":"2004","unstructured":"Anousis, N., Carvalho, R. A., Zhao, P., Malloy, C. R., & Sherry, A. D. (2004). Compartmentation of glycolysis and glycogenolysis in the perfused rat heart. NMR in Biomedicine, 17, 51\u201359.","journal-title":"NMR in Biomedicine"},{"key":"9112_CR36","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.radonc.2009.06.025","volume":"92","author":"O Feron","year":"2009","unstructured":"Feron, O. (2009). Pyruvate into lactate and back: From the Warburg effect to symbiotic energy fuel exchange in cancer cells. Radiotherapy & Oncology, 92, 329\u2013333.","journal-title":"Radiotherapy & Oncology"},{"key":"9112_CR37","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1038\/nchembio.301","volume":"6","author":"AL Bracha","year":"2010","unstructured":"Bracha, A. L., Ramanathan, A., Huang, S., Ingber, D. E., & Schreiber, S. L. (2010). Carbon metabolism-mediated myogenic differentiation. Nat Chem Biol, 6, 202\u2013204.","journal-title":"Nat Chem Biol"},{"key":"9112_CR38","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/S0925-4439(02)00144-8","volume":"1588","author":"PS Green","year":"2002","unstructured":"Green, P. S., & Leeuwenburgh, C. (2002). Mitochondrial dysfunction is an early indicator of doxorubicin-induced apoptosis. Biochimica et Biophysica Acta, 1588, 94\u2013101.","journal-title":"Biochimica et Biophysica Acta"},{"key":"9112_CR39","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1007\/s12012-010-9062-2","volume":"10","author":"JP Silva","year":"2010","unstructured":"Silva, J. P., Sardao, V. A., Coutinho, O. P., & Oliveira, P. J. (2010). Nitrogen compounds prevent h9c2 myoblast oxidative stress-induced mitochondrial dysfunction and cell death. Cardiovascular Toxicology, 10, 51\u201365.","journal-title":"Cardiovascular Toxicology"}],"container-title":["Cardiovascular Toxicology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s12012-011-9112-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s12012-011-9112-4\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s12012-011-9112-4","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,6,9]],"date-time":"2019-06-09T07:33:45Z","timestamp":1560065625000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s12012-011-9112-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,3,24]]},"references-count":39,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2011,6]]}},"alternative-id":["9112"],"URL":"https:\/\/doi.org\/10.1007\/s12012-011-9112-4","relation":{},"ISSN":["1530-7905","1559-0259"],"issn-type":[{"value":"1530-7905","type":"print"},{"value":"1559-0259","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,3,24]]}}}