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These organisms perform extracellular electron transfer (EET), which constitutes the foundations of bioelectrochemical systems for the production of biofuels and bioenergy. It was proposed that the Gram-negative bacterium <jats:italic>Sideroxydans lithotrophicus<\/jats:italic> ES-1 oxidizes soluble ferrous Fe(II) at the surface of the cell and performs EET through the Mto redox pathway. This pathway is composed by the periplasmic monoheme cytochrome MtoD that is proposed to bridge electron transfer between the cell exterior and the cytoplasm. This makes its functional and structural characterization, as well as evaluating the interaction process with its physiological partners, essential for understanding the mechanisms underlying EET. Here, we report the complete assignment of the heme proton and carbon signals together with a near-complete assignment of <jats:sup>1<\/jats:sup>H, <jats:sup>13<\/jats:sup>C and <jats:sup>15<\/jats:sup>N backbone and side chain resonances for the reduced, diamagnetic form of the protein. These data pave the way to identify and structurally map the molecular interaction regions between the cytochrome MtoD and its physiological redox partners, to explore the EET processes of <jats:italic>S. lithotrophicus<\/jats:italic> ES-1.<\/jats:p>","DOI":"10.1007\/s12104-024-10180-8","type":"journal-article","created":{"date-parts":[[2024,6,7]],"date-time":"2024-06-07T03:32:14Z","timestamp":1717731134000},"page":"139-146","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Resonance assignments of cytochrome MtoD from the extracellular electron uptake pathway of sideroxydans lithotrophicus ES-1"],"prefix":"10.1007","volume":"18","author":[{"given":"Ana\u00edsa","family":"Coelho","sequence":"first","affiliation":[]},{"given":"Jos\u00e9 M.","family":"Silva","sequence":"additional","affiliation":[]},{"given":"Francesca","family":"Cantini","sequence":"additional","affiliation":[]},{"given":"Mario","family":"Piccioli","sequence":"additional","affiliation":[]},{"given":"Ricardo O.","family":"Louro","sequence":"additional","affiliation":[]},{"given":"Catarina M.","family":"Paquete","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2024,6,7]]},"reference":[{"key":"10180_CR1","doi-asserted-by":"crossref","unstructured":"Beckwith CR, Edwards MJ, Lawes M, Shi L, Butt JN, Richardson DJ, Clarke TA (2015) Characterization of MtoD from sideroxydans lithotrophicus: a cytochrome c electron shuttle used in lithoautotrophic growth. 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