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Res."],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Microalgae are lipid-rich microscopic eukaryotic algae that can be used aiming for more sustainable biodiesel production by employing environmentally sound processes. The present work evaluates biodiesel production using a biocatalyst and two microalgae species as oil feedstock (<jats:italic>Chlorella vulgaris<\/jats:italic> and <jats:italic>Aurantiochytrium<\/jats:italic> sp<jats:italic>.<\/jats:italic>). Lipid extraction was performed using different techniques, namely, Soxhlet extractions (8 h \u2014 both species) with different solvents (hexane; hexane:ethanol (1:1 v\/v); and chloroform) and room temperature hexane extraction (72 h \u2014 <jats:italic>Aurantiochytrium<\/jats:italic> sp.). Transesterification occurred for 24 h (150 rpm), using 30\u00a0% lipase loading. The results showed that high extraction temperatures cause microalgae oil degradation, focused on unsaturated fatty acids, leading to a lower biodiesel conversion yield. Using <jats:italic>Aurantiochytrium<\/jats:italic> sp. oil, it was possible to obtain around 55 %wt. of biodiesel conversion yield using oil extracted at room temperature (6:1 methanol:oil molar ratio), whereas for the oil extracted in the Soxhlet apparatus, the biodiesel conversion yield was around 30 %wt.. The low lipid content (1.0 %wt.) and biodiesel conversion yield (up to 25 %wt.) obtained using <jats:italic>C. vulgaris<\/jats:italic> show that the biomass used in the current study has low potential for biodiesel production. However, enzymatic biodiesel production from microalgae represents a promising avenue for sustainable energy generation, offering a renewable and environmentally responsible solution to the world\u2019s energy needs. For that purpose, further studies, such as the optimisation of the extraction and transesterification of <jats:italic>Aurantiochytrium<\/jats:italic> sp. oil, should be carried out.<\/jats:p>","DOI":"10.1007\/s12155-023-10706-3","type":"journal-article","created":{"date-parts":[[2023,12,8]],"date-time":"2023-12-08T21:44:37Z","timestamp":1702071877000},"page":"1080-1089","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Biodiesel Production by Biocatalysis using Lipids Extracted from Microalgae Oil of Chlorella vulgaris and Aurantiochytrium sp."],"prefix":"10.1007","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1466-2182","authenticated-orcid":false,"given":"Joana","family":"Oliveira","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4996-1668","authenticated-orcid":false,"given":"Emanuel","family":"Costa","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0966-0537","authenticated-orcid":false,"given":"Joana","family":"Maia Dias","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2738-5102","authenticated-orcid":false,"given":"Jos\u00e9 C.","family":"Pires","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2023,12,8]]},"reference":[{"issue":"2","key":"10706_CR1","doi-asserted-by":"publisher","first-page":"557","DOI":"10.1016\/j.rser.2009.10.009","volume":"14","author":"L Brennan","year":"2010","unstructured":"Brennan L, Owende P (2010) Biofuels from microalgae\u2014a review of technologies for production, processing, and extractions of biofuels and co-products. 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