{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T13:14:38Z","timestamp":1773753278472,"version":"3.50.1"},"reference-count":33,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2009,9,1]],"date-time":"2009-09-01T00:00:00Z","timestamp":1251763200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biophys Rev"],"published-print":{"date-parts":[[2009,9]]},"DOI":"10.1007\/s12551-009-0016-5","type":"journal-article","created":{"date-parts":[[2009,9,3]],"date-time":"2009-09-03T03:32:55Z","timestamp":1251948775000},"page":"141-148","source":"Crossref","is-referenced-by-count":32,"title":["Fluorescent membrane probes\u2019 behavior in lipid bilayers: insights from molecular dynamics simulations"],"prefix":"10.1007","volume":"1","author":[{"given":"Lu\u00eds M. S.","family":"Loura","sequence":"first","affiliation":[]},{"given":"J. P.","family":"Prates Ramalho","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2009,9,4]]},"reference":[{"key":"16_CR1","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.bbamem.2004.04.012","volume":"1666","author":"WL Ash","year":"2004","unstructured":"Ash WL, Zlomislic MR, Oloo EO, Tieleman DP (2004) Computer simulations of membrane proteins. Biochim Biophys Acta 1666:158\u2013189","journal-title":"Biochim Biophys Acta"},{"key":"16_CR2","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1016\/S0006-3495(79)85271-6","volume":"26","author":"D Axelrod","year":"1979","unstructured":"Axelrod D (1979) Carbocyanine dye orientation in red cell membrane studied by microscopic fluorescence polarization. Biophys J 26:557\u2013573","journal-title":"Biophys J"},{"key":"16_CR3","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.bbamem.2008.09.009","volume":"1788","author":"ML Berkowitz","year":"2009","unstructured":"Berkowitz ML (2009) Detailed molecular dynamics simulations of model biological membranes containing cholesterol. Biochim Biophys Acta 1788:86\u201396","journal-title":"Biochim Biophys Acta"},{"key":"16_CR4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0009-3084(90)90128-E","volume":"53","author":"A Chattopadhyay","year":"1990","unstructured":"Chattopadhyay A (1990) Chemistry and biology of N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl)-labeled lipids: fluorescent probes of biological and model membranes. Chem Phys Lipids 53:1\u201315","journal-title":"Chem Phys Lipids"},{"key":"16_CR5","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1021\/bi00375a006","volume":"26","author":"A Chattopadhyay","year":"1987","unstructured":"Chattopadhyay A, London E (1987) Parallax method for direct measurement of membrane penetration depth utilizing fluorescence quenching by spin-labeled phospholipids. Biochemistry 26:39\u201345","journal-title":"Biochemistry"},{"key":"16_CR6","doi-asserted-by":"crossref","first-page":"3640","DOI":"10.1021\/jp065956w","volume":"111","author":"J \u010curdov\u00e1","year":"2007","unstructured":"\u010curdov\u00e1 J, \u010capkov\u00e1 P, Pl\u00e1\u0161ek J, Rep\u00e1kov\u00e1 J, Vattulainen I (2007) Free pyrene probes in gel and fluid membranes: perspective through atomistic simulations. J Phys Chem B 111:3640\u20133650","journal-title":"J Phys Chem B"},{"key":"16_CR7","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.chemphyslip.2008.07.011","volume":"157","author":"RF Almeida de","year":"2009","unstructured":"de Almeida RF, Loura LMS, Prieto M (2009) Membrane lipid domains and rafts: current applications of fluorescence lifetime spectroscopy and imaging. Chem Phys Lipids 157:61\u201377","journal-title":"Chem Phys Lipids"},{"key":"16_CR8","doi-asserted-by":"crossref","DOI":"10.1007\/978-1-4757-2065-5","volume-title":"Biomembranes: molecular structure and function","author":"RB Gennis","year":"1989","unstructured":"Gennis RB (1989) Biomembranes: molecular structure and function. Springer, New York"},{"key":"16_CR9","doi-asserted-by":"crossref","first-page":"3548","DOI":"10.1039\/b716979e","volume":"10","author":"RR Gullapalli","year":"2008","unstructured":"Gullapalli RR, Demirel MC, Butler PJ (2008) Molecular dynamics simulations of DiI-C18(3) in a DPPC lipid bilayer. Phys Chem Chem Phys 10:3548\u20133560","journal-title":"Phys Chem Chem Phys"},{"key":"16_CR10","volume-title":"Biosorption of Polycyclic Aromatic Hydrocarbons (PAH) to microorganisms and liposomes","author":"M Herrenbauer","year":"2002","unstructured":"Herrenbauer M (2002) Biosorption of Polycyclic Aromatic Hydrocarbons (PAH) to microorganisms and liposomes. Shaker, Aachen"},{"key":"16_CR11","doi-asserted-by":"crossref","first-page":"1818","DOI":"10.1529\/biophysj.104.052399","volume":"88","author":"B Hoff","year":"2005","unstructured":"Hoff B, Strandberg E, Ulrich AS, Tieleman DP, Posten C (2005) 2H-NMR study and molecular dynamics simulation of the location, alignment, and mobility of pyrene in POPC bilayers. Biophys J 88:1818\u20131827","journal-title":"Biophys J"},{"key":"16_CR12","doi-asserted-by":"crossref","first-page":"1839","DOI":"10.1111\/j.1600-0854.2008.00801.x","volume":"9","author":"M H\u00f6ltt\u00e4-Vuori","year":"2008","unstructured":"H\u00f6ltt\u00e4-Vuori M, Uronen RL, Rep\u00e1kov\u00e1 J, Salonen E, Vattulainen I, Panula P, Li Z, Bittman R, Ikonen E (2008) BODIPY-cholesterol: a new tool to visualize sterol trafficking in living cells and organisms. Traffic 9:1839\u20131849","journal-title":"Traffic"},{"key":"16_CR13","doi-asserted-by":"crossref","first-page":"822","DOI":"10.1016\/S0006-3495(01)76061-4","volume":"80","author":"D Huster","year":"2001","unstructured":"Huster D, M\u00fcller P, Arnold K, Herrmann A (2001) Dynamics of membrane penetration of the fluorescent 7-nitrobenz-2-oxa-1, 3-diazol-4-yl (NBD) group attached to an acyl chain of phosphatidylcholine. Biophys J 80:822\u2013831","journal-title":"Biophys J"},{"key":"16_CR14","doi-asserted-by":"crossref","first-page":"8180","DOI":"10.1021\/bi980064a","volume":"37","author":"RD Kaiser","year":"1998","unstructured":"Kaiser RD, London E (1998) Location of diphenylhexatriene (DPH) and its derivatives within membranes: comparison of different fluorescence quenching analyses of membrane depth. Biochemistry 37:8180\u20138190","journal-title":"Biochemistry"},{"key":"16_CR15","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/0009-3084(89)90049-2","volume":"50","author":"BR Lentz","year":"1989","unstructured":"Lentz BR (1989) Membrane \u201cfluidity\u201d as detected by diphenylhexatriene probes. Chem Phys Lipids 50:171\u2013190","journal-title":"Chem Phys Lipids"},{"key":"16_CR16","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/0009-3084(93)90060-G","volume":"64","author":"BR Lentz","year":"1993","unstructured":"Lentz BR (1993) Use of fluorescence probes to monitor molecular order and motions within liposome bilayers. Chem Phys Lipids 64:99\u2013116","journal-title":"Chem Phys Lipids"},{"key":"16_CR17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0301-4622(96)02267-3","volume":"69","author":"JJ L\u00f3pez Cascales","year":"1997","unstructured":"L\u00f3pez Cascales JJ, Huertas ML, Garc\u00eda de la Torre J (1997) Molecular dynamics simulation of a dye molecule in the interior of a bilayer: 1, 6-diphenyl-1, 3, 5-hexatriene in dipalmitoylphosphatidylcholine. Biophys Chem 69:1\u20138","journal-title":"Biophys Chem"},{"key":"16_CR18","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1016\/j.bbamem.2006.10.011","volume":"1768","author":"LMS Loura","year":"2007","unstructured":"Loura LMS, Prates Ramalho JP (2007) Location and dynamics of acyl chain NBD-labeled phosphatidylcholine (NBD-PC) in DPPC bilayers. A molecular dynamics and time-resolved fluorescence anisotropy study. Biochim Biophys Acta 1768:467\u2013478","journal-title":"Biochim Biophys Acta"},{"key":"16_CR19","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/S0005-2736(01)00269-3","volume":"1511","author":"LMS Loura","year":"2001","unstructured":"Loura LMS, Fedorov A, Prieto M (2001) Exclusion of a cholesterol analog from the cholesterol-rich phase in model membranes. Biochim Biophys Acta 1511:236\u2013243","journal-title":"Biochim Biophys Acta"},{"key":"16_CR20","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/j.bbamem.2007.10.022","volume":"1778","author":"LMS Loura","year":"2008","unstructured":"Loura LMS, Fernandes F, Fernandes AC, Prates Ramalho JP (2008) Effects of fluorescent probe NBD-PC on the structure, dynamics and phase transition of DPPC. A molecular dynamics and differential scanning calorimetry study. Biochim Biophys Acta 1778:491\u2013501","journal-title":"Biochim Biophys Acta"},{"key":"16_CR21","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0009-3084(02)00017-8","volume":"116","author":"O Maier","year":"2002","unstructured":"Maier O, Oberle V, Hoekstra D (2002) Fluorescent lipid probes: some properties and applications (a review). Chem Phys Lipids 116:3\u201318","journal-title":"Chem Phys Lipids"},{"key":"16_CR22","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1016\/S0006-3495(98)74011-1","volume":"74","author":"DC Mitchell","year":"1998","unstructured":"Mitchell DC, Litman BJ (1998) Molecular order and dynamics in bilayers consisting of highly polyunsaturated phospholipids. Biophys J 74:879\u2013891","journal-title":"Biophys J"},{"key":"16_CR23","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.chemphyslip.2003.09.004","volume":"127","author":"S Mukherjee","year":"2004","unstructured":"Mukherjee S, Raghuraman H, Dasgupta S, Chattopadhyay A (2004) Organization and dynamics of N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl)-labeled lipids: a fluorescence approach. Chem Phys Lipids 127:91\u2013101","journal-title":"Chem Phys Lipids"},{"key":"16_CR24","doi-asserted-by":"crossref","first-page":"13438","DOI":"10.1021\/jp048381g","volume":"108","author":"J Rep\u00e1kov\u00e1","year":"2004","unstructured":"Rep\u00e1kov\u00e1 J, \u010capkov\u00e1 P, Holopainen JM, Vattulainen I (2004) Distribution, orientation, and dynamics of DPH probes in DPPC bilayer. J Phys Chem B 108:13438\u201313448","journal-title":"J Phys Chem B"},{"key":"16_CR25","doi-asserted-by":"crossref","first-page":"3398","DOI":"10.1529\/biophysj.104.055533","volume":"88","author":"J Rep\u00e1kov\u00e1","year":"2005","unstructured":"Rep\u00e1kov\u00e1 J, Holopainen JM, Morrow MR, McDonald MC, \u010capkov\u00e1 P, Vattulainen I (2005) Influence of DPH on the structure and dynamics of a DPPC bilayer. Biophys J 88:3398\u20133410","journal-title":"Biophys J"},{"key":"16_CR26","doi-asserted-by":"crossref","first-page":"15403","DOI":"10.1021\/jp061300r","volume":"110","author":"J Rep\u00e1kov\u00e1","year":"2006","unstructured":"Rep\u00e1kov\u00e1 J, Holopainen JM, Karttunen M, Vattulainen I (2006) Influence of pyrene-labeling on fluid lipid membranes. J Phys Chem B 110:15403\u201315410","journal-title":"J Phys Chem B"},{"key":"16_CR27","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/S0076-6879(08)03405-8","volume":"450","author":"CA Royer","year":"2008","unstructured":"Royer CA, Scarlata SF (2008) Fluorescence approaches to quantifying biomolecular interactions. Methods Enzymol 450:79\u2013106","journal-title":"Methods Enzymol"},{"key":"16_CR28","doi-asserted-by":"crossref","first-page":"2170","DOI":"10.1529\/biophysj.105.073510","volume":"90","author":"JE Shaw","year":"2006","unstructured":"Shaw JE, Epand RF, Epand RM, Li Z, Bittman R, Yip CM (2006) Correlated fluorescence-atomic force microscopy of membrane domains: structure of fluorescence probes determines lipid localization. Biophys J 90:2170\u20132178","journal-title":"Biophys J"},{"key":"16_CR29","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1038\/42408","volume":"387","author":"K Simons","year":"1997","unstructured":"Simons K, Ikonen E (1997) Functional rafts in cell membranes. Nature 387:569\u2013572","journal-title":"Nature"},{"key":"16_CR30","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/S0009-3084(02)00020-8","volume":"116","author":"P Somerharju","year":"2002","unstructured":"Somerharju P (2002) Pyrene-labeled lipids as tools in membrane biophysics and cell biology. Chem Phys Lipids 116:57\u201374","journal-title":"Chem Phys Lipids"},{"key":"16_CR31","doi-asserted-by":"crossref","first-page":"5113","DOI":"10.1021\/bi00390a033","volume":"26","author":"M Straume","year":"1987","unstructured":"Straume M, Litman BJ (1987) Equilibrium and dynamic structure of large, unilamellar, unsaturated acyl chain phosphatidylcholine vesicles. Higher order analysis of 1, 6-diphenyl-1, 3, 5-hexatriene and 1-[4-(trimethylammonio)phenyl]- 6-phenyl-1, 3, 5-hexatriene anisotropy decay. Biochemistry 26:5113\u20135120 Erratum in: Biochemistry 26:8030","journal-title":"Biochemistry"},{"key":"16_CR32","first-page":"193","volume-title":"Spectroscopic membrane probes, vol I","author":"DE Wolf","year":"1988","unstructured":"Wolf DE (1988) Probing the lateral organization and dynamics of membranes. In: Loew LM (ed) Spectroscopic membrane probes, vol I. CRC, Boca Raton, pp 193\u2013220"},{"key":"16_CR33","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.bbamem.2008.07.030","volume":"1788","author":"Y Zhang","year":"2009","unstructured":"Zhang Y, Li X, Becker KA, Gulbins E (2009) Ceramide-enriched membrane domains - structure and function. Biochim Biophys Acta 1788:178\u2013183","journal-title":"Biochim Biophys Acta"}],"container-title":["Biophysical Reviews"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s12551-009-0016-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s12551-009-0016-5\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s12551-009-0016-5","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,6,2]],"date-time":"2019-06-02T08:19:08Z","timestamp":1559463548000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s12551-009-0016-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,9]]},"references-count":33,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2009,9]]}},"alternative-id":["16"],"URL":"https:\/\/doi.org\/10.1007\/s12551-009-0016-5","relation":{},"ISSN":["1867-2450","1867-2469"],"issn-type":[{"value":"1867-2450","type":"print"},{"value":"1867-2469","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,9]]}}}