{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:33:54Z","timestamp":1772120034978,"version":"3.50.1"},"reference-count":15,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2025,5,5]],"date-time":"2025-05-05T00:00:00Z","timestamp":1746403200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,5,5]],"date-time":"2025-05-05T00:00:00Z","timestamp":1746403200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Senckenberg Gesellschaft f\u00fcr Naturforschung (SGN)"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Conservation Genet Resour"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Anthropogenic hybridisation between wild and domestic taxa poses a significant threat to species integrity, including the endangered European wildcat. To enable reliable molecular assessment of admixture with domestic cats and to increase the accuracy of hybrid class assignment we optimised an existing reduced microfluidic 96 Single Nucleotide Polymorphism (SNP) panel. We selected SNPs from a genome-wide dataset for maximum F\n                    <jats:sub>ST<\/jats:sub>\n                    between both taxa and replaced 60 SNPs from the previous 96 SNP panel. Comparison of both panels based on simulated hybrid genotypes and real-world genotypes proof the higher discriminatory power of the optimised panel, which allows for reliable assignment of F1 and F2 hybrids, as well as 1st and 2nd generation backcrosses. Additionally, we successfully tested the panel for both tissue and non-invasively collected hair samples, demonstrating the suitability of the new panel for implementation in wildcat monitoring programmes.\n                  <\/jats:p>","DOI":"10.1007\/s12686-025-01386-6","type":"journal-article","created":{"date-parts":[[2025,5,5]],"date-time":"2025-05-05T01:37:13Z","timestamp":1746409033000},"page":"127-130","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Optimisation of a microfluidic SNP assay for accurate hybrid class detection in the European wildcat (Felis silvestris)"],"prefix":"10.1007","volume":"17","author":[{"given":"Lina S.","family":"Martin","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1398-7396","authenticated-orcid":false,"given":"Gregor","family":"Rolshausen","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4797-0939","authenticated-orcid":false,"given":"Paulo C.","family":"Alves","sequence":"additional","affiliation":[]},{"given":"Federica","family":"Mattucci","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3641-9490","authenticated-orcid":false,"given":"Romolo","family":"Caniglia","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4882-6595","authenticated-orcid":false,"given":"Ettore","family":"Randi","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3139-1951","authenticated-orcid":false,"given":"Carsten","family":"Nowak","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8427-7861","authenticated-orcid":false,"given":"Berardino","family":"Cocchiararo","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2025,5,5]]},"reference":[{"issue":"11","key":"1386_CR1","doi-asserted-by":"publisher","first-page":"613","DOI":"10.1016\/S0169-5347(01)02290-X","volume":"16","author":"FW Allendorf","year":"2001","unstructured":"Allendorf FW, Leary RF, Spruell P, Wenburg JK (2001) The problems with hybrids: setting conservation guidelines. Trends Ecol Evol 16(11):613\u2013622. https:\/\/doi.org\/10.1016\/S0169-5347(01)02290-X","journal-title":"Trends Ecol Evol"},{"issue":"3","key":"1386_CR2","doi-asserted-by":"publisher","first-page":"1217","DOI":"10.1093\/genetics\/160.3.1217","volume":"160","author":"EC Anderson","year":"2002","unstructured":"Anderson EC, Thompson E (2002) A model-based method for identifying species hybrids using multilocus genetic data. Genetics 160(3):1217\u20131229. https:\/\/doi.org\/10.1093\/genetics\/160.3.1217","journal-title":"Genetics"},{"issue":"11","key":"1386_CR3","doi-asserted-by":"publisher","first-page":"1403","DOI":"10.1093\/bioinformatics\/btn129","volume":"24","author":"T Jombart","year":"2008","unstructured":"Jombart T (2008) Adegenet: a R package for the multivariate analysis of genetic markers. Bioinformatics 24(11):1403\u20131405","journal-title":"Bioinformatics"},{"key":"1386_CR4","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/1471-2156-11-94","volume":"11","author":"T Jombart","year":"2010","unstructured":"Jombart T, Devillard S, Balloux F (2010) Discriminant analysis of principal components: a new method for the analysis of genetically structured populations. BMC Genet 11:1\u201315. https:\/\/doi.org\/10.1186\/1471-2156-11-94","journal-title":"BMC Genet"},{"key":"1386_CR5","unstructured":"Mattucci F (2014) Conservation genetics of European wildcat. (Felis silvestris silvestris): A wide and integrating analysis protocol for admixture inferences and population structure (Unpublished doctoral dissertation). University of Bologna. https:\/\/amsdottorato.unibo.it\/id\/eprint\/6459\/1\/Phd_thesis_MattucciF_170314.pdf"},{"issue":"1","key":"1386_CR9","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1002\/ece3.1815","volume":"6","author":"F Mattucci","year":"2016","unstructured":"Mattucci F, Oliveira R, Lyons LA, Alves PC, Randi E (2016) European wildcat populations are subdivided into five main biogeographic groups: consequences of pleistocene climate changes or recent anthropogenic fragmentation? Ecol Evol 6(1):3\u201322. https:\/\/doi.org\/10.1002\/ece3.1815","journal-title":"Ecol Evol"},{"key":"1386_CR10","doi-asserted-by":"publisher","first-page":"11612","DOI":"10.1038\/s41598-019-48002-w","volume":"9","author":"F Mattucci","year":"2019","unstructured":"Mattucci F, Galaverni M, Lyons LA, Alves PC, Randi E, Velli E, Caniglia R (2019) Genomic approaches to identify hybrids and estimate admixture times in European wildcat populations. 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Eur J Wildl Res 60:405\u2013410. https:\/\/doi.org\/10.1007\/s10344-014-0806-3","journal-title":"Eur J Wildl Res"},{"key":"1386_CR13","doi-asserted-by":"publisher","first-page":"110029","DOI":"10.1016\/j.biocon.2023.110029","volume":"281","author":"B Nussberger","year":"2023","unstructured":"Nussberger B, Hertwig ST, Roth T (2023) Monitoring distribution, density and introgression in European wildcats in Switzerland. Biol Conserv 281:110029. https:\/\/doi.org\/10.1016\/j.biocon.2023.110029","journal-title":"Biol Conserv"},{"key":"1386_CR14","unstructured":"R Core Team (2024). R: A language and environment for statistical computing. 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Mol Ecol Resour 20(3):662\u2013680. https:\/\/doi.org\/10.1111\/1755-0998.13136","journal-title":"Mol Ecol Resour"}],"container-title":["Conservation Genetics Resources"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12686-025-01386-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s12686-025-01386-6\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s12686-025-01386-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,9]],"date-time":"2025-06-09T12:02:20Z","timestamp":1749470540000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s12686-025-01386-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,5]]},"references-count":15,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2025,6]]}},"alternative-id":["1386"],"URL":"https:\/\/doi.org\/10.1007\/s12686-025-01386-6","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-4902208\/v1","asserted-by":"object"}]},"ISSN":["1877-7260"],"issn-type":[{"value":"1877-7260","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,5]]},"assertion":[{"value":"12 August 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 April 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 May 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}