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Compare POL <jats:italic>vs<\/jats:italic> threshold (THR) training on V\u0307O<jats:sub>2<\/jats:sub>max, endurance capacity (EC) and mitochondrial function. Fifteen male Wistar rats (336.1\u2009\u00b1\u200930.4\u00a0g) were divided in: POL (<jats:italic>n<\/jats:italic>\u2009=\u20095), THR (<jats:italic>n<\/jats:italic>\u2009=\u20095) or control (CON; <jats:italic>n<\/jats:italic>\u2009=\u20095) groups. V\u0307O<jats:sub>2<\/jats:sub>max (indirect calorimetry) and EC (treadmill exhaustion test) were determined at baseline four and eight-weeks of training. POL consisted of 80% running volume at 60%V\u0307O<jats:sub>2<\/jats:sub>max and 20% at 90%V\u0307O<jats:sub>2<\/jats:sub>max while THR trained only at 75%V\u0307O<jats:sub>2<\/jats:sub>max. Both protocols were isocaloric and performed 5d\/week. All animals were housed in cages with access to running wheel to allow ad libitum activity. After training, animals were sacrificed and left ventricle (LV) myocardium, diaphragm, <jats:italic>tibialis anterior<\/jats:italic> and <jats:italic>soleus<\/jats:italic> muscles were collected for high-resolution respirometry, biochemical and histological analysis. There were no baseline differences between groups. After training V\u0307O<jats:sub>2<\/jats:sub>max and EC were similar between POL and THR even though THR V\u0307O<jats:sub>2<\/jats:sub>max was higher compared to CON. After training, there were also no significant differences in OXPHOS or any of the other major mitochondrial function markers assessed between POL and THR in any of the tissues analyzed. The expression of MFN1, MFN2, PGC-1\u03b1, TFAM, DRP1, OPA1 and TOM20 as well as the activity of citrate synthase were also similar between POL and THR in all tissues. There were no significant differences in endurance performance or markers of bioenergetic function between POL and THR after eight-weeks of training.<\/jats:p>","DOI":"10.1007\/s13105-025-01079-6","type":"journal-article","created":{"date-parts":[[2025,4,4]],"date-time":"2025-04-04T05:01:37Z","timestamp":1743742897000},"page":"329-346","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Polarized or threshold training: is there a superior training intensity distribution to improve V\u0307O2max, endurance capacity and mitochondrial function? 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