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The same aqueous treatment achieved the best performance on <jats:italic>S. obliquus<\/jats:italic>, reaching 82 g\/100 g protein. All treatments on both microalgae partially solubilized the polysaccharide fraction with all alkaline treatments solubilizing over 50 g\/100 g sugars for all conditions. Overall, all the treatments applied were effective methods for biomass fractionation, although they showed low selectivity regarding the individual extraction of protein or carbohydrates.<\/jats:p>","DOI":"10.1007\/s13399-024-05279-w","type":"journal-article","created":{"date-parts":[[2024,1,19]],"date-time":"2024-01-19T01:01:46Z","timestamp":1705626106000},"page":"999-1011","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Evaluation of different fractionation methods for the simultaneous protein and carbohydrate extraction from microalgae"],"prefix":"10.1007","volume":"15","author":[{"given":"Pedro L.","family":"Martins","sequence":"first","affiliation":[]},{"given":"Lu\u00eds C.","family":"Duarte","sequence":"additional","affiliation":[]},{"given":"Helena","family":"Pereira","sequence":"additional","affiliation":[]},{"given":"Alberto","family":"Reis","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0270-4832","authenticated-orcid":false,"given":"Florbela","family":"Carvalheiro","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2024,1,19]]},"reference":[{"key":"5279_CR1","doi-asserted-by":"publisher","first-page":"970028","DOI":"10.3389\/fmicb.2022.970028","volume":"13","author":"S Zhang","year":"2022","unstructured":"Zhang S, Zhang L, Xu G, Li F, Li X (2022) A review on biodiesel production from microalgae: influencing parameters and recent advanced technologies. 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