{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T21:06:55Z","timestamp":1740172015140,"version":"3.37.3"},"reference-count":40,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,2,10]],"date-time":"2021-02-10T00:00:00Z","timestamp":1612915200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2021,2,10]],"date-time":"2021-02-10T00:00:00Z","timestamp":1612915200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"funder":[{"name":"ProDeR, co-financed by the FEADER","award":["Ref. N. 53.590"],"award-info":[{"award-number":["Ref. N. 53.590"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Vegetos"],"published-print":{"date-parts":[[2021,3]]},"DOI":"10.1007\/s42535-020-00184-9","type":"journal-article","created":{"date-parts":[[2021,2,11]],"date-time":"2021-02-11T08:47:56Z","timestamp":1613033276000},"page":"127-137","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Medium-term in vitro conservation of Castanea spp. hybrid clones"],"prefix":"10.1007","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9561-3907","authenticated-orcid":false,"given":"Filomena","family":"Gomes","sequence":"first","affiliation":[]},{"given":"Marta","family":"Clemente","sequence":"additional","affiliation":[]},{"given":"Patricia","family":"Figueiredo","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9503-6126","authenticated-orcid":false,"given":"Rita Louren\u00e7o","family":"Costa","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2021,2,10]]},"reference":[{"key":"184_CR1","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1007\/s11627-016-9753-x","volume":"52","author":"JB Bamberg","year":"2016","unstructured":"Bamberg JB  (2016) In vitro technology at the US Potato Genebank .In Vitro Cell Dev Biol Plant 52: 213\u2013225 https:\/\/doi.org\/10.1007\/s11627-016-9753-x","journal-title":"In Vitro Cell Dev Biol Plant"},{"key":"184_CR2","first-page":"319","volume":"8","author":"S Bekheet","year":"2005","unstructured":"Bekheet S, Taha H, El-Bahr M (2005) Preservation of date palm cultures using encapsulated somatic embryos. Arab J Biotechnol 8: 319\u2013328","journal-title":"Arab J Biotechnol"},{"key":"184_CR3","doi-asserted-by":"publisher","first-page":"605","DOI":"10.1007\/s11627-013-9536-6","volume":"49","author":"M Capuana","year":"2013","unstructured":"Capuana M, Lonardo Di S (2013) In vitro conservation of chestnut (Castanea sativa) by slow growth. In Vitro Cell Dev Biol Plant 49: 605\u2013610. Doi: https:\/\/doi.org\/10.1007\/s11627-013-9536-6","journal-title":"In Vitro Cell Dev Biol Plant"},{"key":"184_CR4","first-page":"97","volume":"38","author":"R Charoensub","year":"2004","unstructured":"Charoensub R, Phansiri S (2004) In vitro conservation of Rose coloured leadwort: effect of mannitol on growth of plantlets. Kasetsart J (Nat Sci) 38: 97\u2013102","journal-title":"Kasetsart J (Nat Sci)"},{"key":"184_CR5","doi-asserted-by":"publisher","first-page":"326","DOI":"10.1007\/s11627-014-9600-x","volume":"50","author":"SZ Cordeiro","year":"2014","unstructured":"Cordeiro SZ, Simas NK, Henriques AB, Sato A (2014) In vitro conservation of Mandevilla moricandiana (Apocynaceae): short-term storage and encapsulation\u2013dehydration of nodal segments. In Vitro Cell Dev Biol Plant 50: 326\u2013336. Doi: https:\/\/doi.org\/10.1007\/s11627-014-9600-x","journal-title":"In Vitro Cell Dev Biol Plant"},{"key":"184_CR6","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1080\/00049158.1974.10675595","volume":"37","author":"RA De Fossard","year":"1974","unstructured":"Fossard De RA (1974) Tissue culture of Eucalyptus. Aust For 37: 43\u201354: Doi: https:\/\/doi.org\/10.1080\/00049158.1974.10675595","journal-title":"Aust For"},{"key":"184_CR7","doi-asserted-by":"publisher","first-page":"1","DOI":"10.2307\/3001478","volume":"11","author":"DB Duncan","year":"1955","unstructured":"Duncan DB (1955) Multiple range and multiple F tests. Biometrics 11: 1\u201342. Doi:  https:\/\/doi.org\/10.2307\/3001478","journal-title":"Biometrics"},{"key":"184_CR8","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1007\/s11627-010-9327-2","volume":"47","author":"F Engelmann","year":"2011","unstructured":"Engelmann F (2011) Use of biotechnologies for the conservation of plant biodiversity. In Vitro Cell Dev Biol Plant 47: 5\u201316. Doi:  https:\/\/doi.org\/10.1007\/s11627-010-9327-2","journal-title":"In Vitro Cell Dev Biol Plant"},{"key":"184_CR9","doi-asserted-by":"publisher","first-page":"31","DOI":"10.17660\/ActaHortic.2014.1039.2","volume":"1039","author":"F Engelmann","year":"2014","unstructured":"Engelmann F (2014) Cryopreservation of clonal crops a review of key parameters. ISHS Acta Horticult 1039: 31\u201339. Doi:  https:\/\/doi.org\/10.17660\/ActaHortic.2014.1039.2","journal-title":"ISHS Acta Horticult"},{"key":"184_CR10","doi-asserted-by":"publisher","first-page":"178","DOI":"10.1007\/s11627-001-0031-0","volume":"37","author":"TR Ganapathi","year":"2001","unstructured":"Ganapathi TR, Srinivas L, Suprasanna P, Bapat VA (2001) Regeneration of plants from alginate-encapsulated somatic embryos of banana cv. Rasthali (Musa SPP. AAB Group). In Vitro Cell Dev Biol Plant 37: 178 181. Doi:  https:\/\/doi.org\/10.1007\/s11627-001-0031-0","journal-title":"In Vitro Cell Dev Biol Plant"},{"key":"184_CR11","doi-asserted-by":"publisher","first-page":"37","DOI":"10.17660\/ActaHortic.2017.1155.4","volume":"1039","author":"F Gomes","year":"2015","unstructured":"Gomes F, Clemente M, Figueiredo P, Pl\u00e1cito F, Machado H, Santos C, Costa R (2015) Castanea spp hybrid clones in vitro conservation: Synthetic seeds vs slow growth storage. ISHS Acta Horticulturae 1039: 37\u201344. Doi:  https:\/\/doi.org\/10.17660\/ActaHortic.2017.1155.4","journal-title":"ISHS Acta Horticulturae"},{"key":"184_CR12","doi-asserted-by":"publisher","DOI":"10.9755\/ejfa.v23i2.6344","author":"SM Jain","year":"2011","unstructured":"Jain SM (2011) Prospects of in vitro conservation of date palm genetic diversity for sustainable production. Emir J Food Agric. Doi:  https:\/\/doi.org\/10.9755\/ejfa.v23i2.6344","journal-title":"Emir J Food Agric"},{"key":"184_CR13","doi-asserted-by":"publisher","first-page":"31","DOI":"10.17660\/ActaHortic.2018.1224.5","volume":"1224","author":"P Jourdan","year":"2018","unstructured":"Jourdan P (2018) Short- and medium-term in vitro conservation and management of germplasm within the USDA's national plant germplasm system. ISHS Acta Horticulturae 1224: 31\u201338. Doi:  https:\/\/doi.org\/10.17660\/ActaHortic.2018.1224.5","journal-title":"ISHS Acta Horticulturae"},{"key":"184_CR14","doi-asserted-by":"publisher","first-page":"311","DOI":"10.17660\/ActaHortic.2006.725.38","volume":"725","author":"M Lambardi","year":"2004","unstructured":"Lambardi M, Benelli C, Carlo De A, Previati A, Re Da F, Giannini M (2004) Biotechnologies for the preservation of selected red chicory (Cichorium intybus L.) lines. ISHS Acta Horticulturae 725: 311\u2013318. Doi:  https:\/\/doi.org\/10.17660\/ActaHortic.2006.725.38","journal-title":"ISHS Acta Horticulturae"},{"key":"184_CR15","unstructured":"Lambardi M, Benelli C, Ozudogru EA, Ozden-Tokatli Y (2006) Synthetic seed technology in ornamental plants. In: Silva J eds. Floriculture ornamental and plant biotechnology, vol II, 1st edn, pp 347\u2013354. Kagawa: Global Science Books Ltd"},{"key":"184_CR16","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1007\/s11627-009-9243-5","volume":"46","author":"H Lata","year":"2010","unstructured":"Lata H, Moraes RM, Bertoni B, Pereira AMS (2010) In vitro germplasm conservation of Podophyllum peltatum L. under slow growth conditions. In Vitro Cell Dev Biol Plant 46: 22\u201327. Doi:  https:\/\/doi.org\/10.1007\/s11627-009-9243-5","journal-title":"In Vitro Cell Dev Biol Plant"},{"key":"184_CR17","unstructured":"Matsumoto KC, L.D. (2016) Effects of sucrose and incubation temperature on in vitro conservation of Cassa and Brazilian Ginseng germplasms. Institute ISfHSHR (ed), 83\u201384"},{"key":"184_CR18","doi-asserted-by":"publisher","first-page":"26","DOI":"10.4067\/S0718-58392019000100026","volume":"79","author":"M Mu\u00f1oz","year":"2019","unstructured":"Mu\u00f1oz M, D\u00edaz O, Rein\u00fan W, Winkler A, Quevedo R (2019) Slow growth in vitro culture for conservation of Chilotanum potato germplasm. Chilean J Agric Res 79: 26\u201335. Doi:  https:\/\/doi.org\/10.4067\/S0718-58392019000100026","journal-title":"Chilean J Agric Res"},{"key":"184_CR19","doi-asserted-by":"publisher","first-page":"473","DOI":"10.1111\/j.1399-3054.1962.tb08052.x","volume":"15","author":"T Murashige","year":"1962","unstructured":"Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15: 473\u2013497. Doi:  https:\/\/doi.org\/10.1111\/j.1399-3054.1962.tb08052.x","journal-title":"Physiol Plant"},{"key":"184_CR20","doi-asserted-by":"publisher","first-page":"411","DOI":"10.3233\/JBR-180379","volume":"9","author":"S Nin","year":"2019","unstructured":"Nin S, Benelli C, Petrucci  WA, Turchi A, Pecchioli S, Gori M, Giordani E (2019) In vitro propagation and conservation of wild bilberry (Vaccinium myrtillus L.) genotypes collected in the Tuscan Apennines (Italy). J Berry Res 9: 411\u2013430. Doi:  https:\/\/doi.org\/10.3233\/JBR-180379","journal-title":"J Berry Res"},{"key":"184_CR21","first-page":"17","volume":"8","author":"Y Ozden-Tokatli","year":"2008","unstructured":"Ozden-Tokatli Y, Carlo De A, Gumusel F, Pignattelli S, Lambardi M (2008) Development of encapsulation techniques for the production and conservation of synthetic seeds in ornamental plants. Propag Ornam Plant 8: 17\u201322","journal-title":"Propag Ornam Plant"},{"key":"184_CR22","doi-asserted-by":"publisher","first-page":"638","DOI":"10.15835\/nbha4129335","volume":"41","author":"A Ozudogru","year":"2013","unstructured":"Ozudogru A, Silva da DP, Ergun K, Dradi G, Paiva R, Lambardi M (2013) In vitro conservation and cryopreservation of Nandina domestica, an outdoor ornamental shrub. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 41: 638\u2013645. Doi:  https:\/\/doi.org\/10.15835\/nbha4129335","journal-title":"Notulae Botanicae Horti Agrobotanici Cluj-Napoca"},{"key":"184_CR23","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1007\/s11738-017-2372-2","volume":"39","author":"EA Ozudogru","year":"2017","unstructured":"Ozudogru EA, Benelli C, Dradi G, Lambardi M (2017) Effect of culture container and carbohydrate content on in vitro slow growth storage of the cherry rootstock \u2018Gisela\u00ae5\u2019. Acta Physiol Plant 39: 94. Doi:  https:\/\/doi.org\/10.1007\/s11738-017-2372-2","journal-title":"Acta Physiol Plant"},{"key":"184_CR24","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60327-114-1_28","author":"EA Ozudogru","year":"2010","unstructured":"Ozudogru EA, Previati A, Lambardi M (2010). In vitro conservation and cryopreservation of ornamental plants. Protocols In Vitro Propag Ornam Plant. Doi: https:\/\/doi.org\/10.1007\/978-1-60327-114-1_28","journal-title":"Protocols In Vitro Propag Ornam Plant"},{"key":"184_CR25","doi-asserted-by":"publisher","first-page":"613","DOI":"10.1079\/ivp2003453","volume":"39","author":"C Prewein","year":"2003","unstructured":"Prewein C, Wilhelm E (2003) Plant regeneration from encapsulated somatic embryos of pedunculate oak (Quercus robur L.). In Vitro Cell Dev Biol Plant 39: 613\u2013617. Doi:  https:\/\/doi.org\/10.1079\/ivp2003453","journal-title":"In Vitro Cell Dev Biol Plant"},{"key":"184_CR26","doi-asserted-by":"publisher","first-page":"14254","DOI":"10.5897\/AJB12.770","volume":"11","author":"MC Reddy","year":"2012","unstructured":"Reddy MC, Murthy KSR, Pullaiah T (2012) Synthetic seeds: a review in agriculture and forestry. Afr J Biotech 11: 14254\u201314275. Doi:  https:\/\/doi.org\/10.5897\/AJB12.770","journal-title":"Afr J Biotech"},{"key":"184_CR27","doi-asserted-by":"publisher","first-page":"268","DOI":"10.1139\/W10-001","volume":"56","author":"DL Richter","year":"2010","unstructured":"Richter DL, Kangas LC, Smith JK, Laks Pe 2010 Comparison of effectiveness of wood decay fungi maintained by annual subculture on agar and stored in sterile water for 18 years Can J Microbiol 56 268 271 https:\/\/doi.org\/10.1139\/W10-001","journal-title":"Can J Microbiol"},{"key":"184_CR28","doi-asserted-by":"publisher","first-page":"71","DOI":"10.3390\/agronomy7040071","volume":"7","author":"H Rihan","year":"2017","unstructured":"H Rihan F Kareem M El-Mahrouk M Fuller 2017 Artificial seeds (Principle, aspects and applications) Agronomy 7 71 https:\/\/doi.org\/10.3390\/agronomy7040071","journal-title":"Agronomy"},{"key":"184_CR29","unstructured":"Ruta C, Lambardi M, Tagarelli A, De Mastro G (2016) Tissue culture technologies to preserve Chickpea (Cicer arietinum L.) germplasm. International Society for Horticultural Science (ISHS) & Horticulture Research Institute (HRI) 85\u201386"},{"key":"184_CR30","doi-asserted-by":"publisher","first-page":"901","DOI":"10.1111\/ppa.12313","volume":"64","author":"C Santos","year":"2015","unstructured":"Santos C, Machado H, Correia I, Gomes F, Gomes-Laranjo J, Costa R (2015). Phenotyping Castanea hybrids for Phytophthora cinnamomi resistance Plant Pathol 64: 901\u2013910. Doi:  https:\/\/doi.org\/10.1111\/ppa.12313","journal-title":"Plant Pathol"},{"key":"184_CR31","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1023\/A:1018309300121","volume":"102","author":"D Sarkar","year":"1998","unstructured":"Sarkar D, Naik PS (1998) Factors affecting minimal growth conservation of potato microplants in vitro. Euphytica 102: 275\u2013280. Doi:  https:\/\/doi.org\/10.1023\/A:1018309300121","journal-title":"Euphytica"},{"key":"184_CR32","first-page":"372","volume":"2","author":"R Shibli","year":"2006","unstructured":"R Shibli M Shatnawi W Subaih M Ajlouni 2006 In vitro conservation and cryopreservation of plant genetic resources: a review World J Agric Sci 2 372 382","journal-title":"World J Agric Sci"},{"key":"184_CR33","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1079\/IVP2005735","volume":"42","author":"AK Singh","year":"2006","unstructured":"Singh AK, Sharma M, Varshney R, Agarwal SS, Bansal KC (2006) Plant regeneration from alginate-encapsulated shoot tips of Phylianthus amarus schum and thonn, a medicinally important plant species. In Vitro Cell Dev Biol Plant 42: 109\u2013113. Doi:  https:\/\/doi.org\/10.1079\/IVP2005735","journal-title":"In Vitro Cell Dev Biol Plant"},{"key":"184_CR34","doi-asserted-by":"publisher","first-page":"543","DOI":"10.1007\/978-981-10-4768-8_27","volume-title":"Developments in fungal biology and applied mycology","author":"SK Singh","year":"2017","unstructured":"Singh SK (2017) Ex situ conservation of fungi: a review Developments in fungal biology and applied mycology, p 543\u2013562. Singapore: Springer. Doi:  https:\/\/doi.org\/10.1007\/978-981-10-4768-8_27"},{"key":"184_CR35","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1016\/j.envexpbot.2007.02.004","volume":"61","author":"I Slama","year":"2007","unstructured":"Slama I, Ghnaya T, Hessini K, Messedi D, Savour\u00e9 A, Abdelly C (2007) Comparative study of the effects of mannitol and PEG osmotic stress on growth and solute accumulation in Sesuvium portulacastrum. Environ Exp Bot 61: 10\u201317. Doi:  https:\/\/doi.org\/10.1016\/j.envexpbot.2007.02.004","journal-title":"Environ Exp Bot"},{"key":"184_CR36","doi-asserted-by":"publisher","first-page":"619","DOI":"10.1007\/s11627-015-9706-9","volume":"51","author":"S Thakur","year":"2015","unstructured":"Thakur S, Tiwari KL, Jadhav SK (2015) In vitro approaches for conservation of Asparagus racemosus Willd. In Vitro Cell Dev Biol Plant 51: 619\u2013625. Doi:  https:\/\/doi.org\/10.1007\/s11627-015-9706-9","journal-title":"In Vitro Cell Dev Biol Plant"},{"key":"184_CR37","doi-asserted-by":"publisher","first-page":"915","DOI":"10.1126\/science.1230935","volume":"339","author":"C Walters","year":"2013","unstructured":"Walters C, Berjak P, Pammenter N, Kennedy K, Raven P (2013) Preservation of recalcitrant seeds. Science 339 915 916 https:\/\/doi.org\/10.1126\/science.1230935","journal-title":"Science"},{"key":"184_CR38","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1007\/s11240-006-9155-6","volume":"87","author":"TP West","year":"2006","unstructured":"West TP, Ravindra M, Preece JE (2006) Encapsulation, cold storage, and growth of Hibiscus moscheutos nodal segments. Plant Cell Tissue Organ Cult 87: 223\u2013231. Doi: https:\/\/doi.org\/10.1007\/s11240-006-9155-6","journal-title":"Plant Cell Tissue Organ Cult"},{"key":"184_CR39","unstructured":"Yahyaoui EC, Frasheri D, D\u2019Onghia AM, German\u00e0 A. (2016) Encapsulation and evaluation of some growth regulators effects on in vitro-derived micro-cuttings of three Italian Ficus carica L. genotypes. International Society for Horticultural Science (ISHS) & Horticulture Research Institute (HRI) 87"},{"key":"184_CR40","volume-title":"Biostatistical analysis","author":"J Zar","year":"1996","unstructured":"Zar J (1996) Biostatistical analysis 3. London: Prentice Hall International","edition":"3"}],"container-title":["Vegetos"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s42535-020-00184-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s42535-020-00184-9\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s42535-020-00184-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T12:26:00Z","timestamp":1614601560000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s42535-020-00184-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,2,10]]},"references-count":40,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,3]]}},"alternative-id":["184"],"URL":"https:\/\/doi.org\/10.1007\/s42535-020-00184-9","relation":{},"ISSN":["2229-4473"],"issn-type":[{"type":"electronic","value":"2229-4473"}],"subject":[],"published":{"date-parts":[[2021,2,10]]},"assertion":[{"value":"10 June 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 December 2020","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 December 2020","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 February 2021","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflicts of interest"}},{"value":"Data transparency.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Availability of data and material"}}]}}