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No differences in IVM rates (fresh IVM: 63.16% and IVM post-VIT: 59.38%, <jats:italic>p<\/jats:italic>\u2009=\u20090.72) and timings (17.73\u00a0h in fresh IVM, 17.33\u00a0h in IVM post-VIT, <jats:italic>p<\/jats:italic>\u2009=\u20090.72) were observed whether IVM is performed freshly or after vitrification. Meiotic spindles were shorter in VIT\u2009+\u2009IVM (10.47\u00a0\u00b5m vs 11.23\u00a0\u00b5m in IVM and 11.40\u00a0\u00b5m in IVM\u2009+\u2009VIT, <jats:italic>p<\/jats:italic>\u2009=\u20090.012 and <jats:italic>p<\/jats:italic>\u2009=\u20090.043) and wider in IVM\u2009+\u2009VIT (9.37\u00a0\u00b5m vs 8.12\u00a0\u00b5m in IVM and 8.16\u00a0\u00b5m VIT\u2009+\u2009IVM, <jats:italic>p<\/jats:italic>\u2009=\u20090.027 and <jats:italic>p<\/jats:italic>\u2009=\u20090.026). The length-to-width ratio was lower in vitrified groups (IVM\u2009+\u2009VIT: 1.19 and VIT\u2009+\u2009IVM: 1.26) compared to IVM (1.38), <jats:italic>p<\/jats:italic>\u2009=\u20090.013 and <jats:italic>p<\/jats:italic>\u2009=\u20090.014. No differences in multipolar spindle and chromosome misalignment occurrence and cytoplasmic F-actin filament length and density were observed between groups. Our results suggest vitrification before or after rescue-IVM does not seem to impair maturation rates and kinetics parameters but induces meiotic spindle alterations.<\/jats:p>","DOI":"10.1007\/s43032-024-01596-7","type":"journal-article","created":{"date-parts":[[2024,5,21]],"date-time":"2024-05-21T20:17:32Z","timestamp":1716322652000},"page":"2625-2636","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Vitrification of Human Oocytes Before or After Rescue-IVM Does not Impair Maturation Kinetics but Induces Meiotic Spindle Alterations"],"prefix":"10.1007","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0064-571X","authenticated-orcid":false,"given":"Ga\u00eblle","family":"Marteil","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0009-0005-5937-2313","authenticated-orcid":false,"given":"A\u00efcha","family":"Metchat","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3908-6051","authenticated-orcid":false,"given":"Sandra","family":"Dollet","sequence":"additional","affiliation":[]},{"given":"Camille","family":"Cugnot","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2362-3062","authenticated-orcid":false,"given":"Laure","family":"Chaput","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3778-7161","authenticated-orcid":false,"given":"Bruno","family":"Pereira","sequence":"additional","affiliation":[]},{"given":"Anne Sophie","family":"Gremeau","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8402-8734","authenticated-orcid":false,"given":"Florence","family":"Brugnon","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2024,5,21]]},"reference":[{"key":"1596_CR1","doi-asserted-by":"publisher","first-page":"1213","DOI":"10.1016\/S0140-6736(65)90636-7","volume":"2","author":"M Mozes","year":"1965","unstructured":"Mozes M, Bogokowsky H, Antebi E, Lunenfeld B, Rabau E, Serr DM, et al. 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Written and informed consent was obtained for each patient to use their samples in this study (CPP 2.15.27). The Biobank has a declaration DC-2021\u20134820 and an authorization AC-2019\u20133487. The request\u2019s number made to GERMETHEQUE is the 20170202.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical Approval and Consent to Participate"}},{"value":"All authors have approved the manuscript and agreed with its submission to Reproductive Sciences.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for Publication"}},{"value":"Authors have no conflicts of interest\/competing interests to declare.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing Interests"}}]}}