{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T12:27:00Z","timestamp":1762864020415},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2001,10,25]],"date-time":"2001-10-25T00:00:00Z","timestamp":1003968000000},"content-version":"vor","delay-in-days":6611,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[1983,9,19]]},"abstract":"<jats:p>The gene, <jats:italic>penPC<\/jats:italic>, for \u03b2\u2010lactamase I of <jats:italic>Bacillus cereus<\/jats:italic> 569\/H has been cloned and its expression studied in <jats:italic>Escherichia coli<\/jats:italic>. The protein product from the in vitro translation of <jats:italic>penPC<\/jats:italic> was shown by gel electrophoresis to have an <jats:italic>M<\/jats:italic>\n<jats:sub>r<\/jats:sub> of 36 000 which is larger than the in vivo products fround in <jats:italic>B. cereus<\/jats:italic> and <jats:italic>E. coli<\/jats:italic>. The DNA sequence of the signal region was determined. It revealed that the smallest known mature form present in <jats:italic>B. cereus<\/jats:italic> culture fluids is preceded by 45\u201348 amino acids in pre\u2010\u03b2\u2010lactamase I, considering that there are 3 initiation codons in the same reading frame, one or more of which might be initiating translation. Unlike the <jats:italic>Bacillus licheniformis<\/jats:italic> 749\/C \u03b2\u2010lactamase, which has a membrane\u2010bound thioether lipoprotein form, the single Cys residue in the <jats:italic>B. cereus<\/jats:italic> \u03b2\u2010lactamase I signal sequence is unmodified and a single processed form of the enzyme is present in <jats:italic>E. coli<\/jats:italic> cells carrying <jats:italic>pen PC<\/jats:italic>\n<\/jats:p>","DOI":"10.1016\/0014-5793(83)81006-0","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T07:59:22Z","timestamp":1027583962000},"page":"195-200","source":"Crossref","is-referenced-by-count":20,"title":["<i>Bacillus cereus<\/i> 569\/H \u03b2\u2010lactamase I: Cloning in <i>Escherichia coli<\/i> and signal sequence determination"],"prefix":"10.1002","volume":"161","author":[{"given":"Peter S.F.","family":"M\u00e9zes","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue Qin","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Musaddeq","family":"Hussain","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Oliver Lampen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,10,25]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.1980.0049"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00550a023"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1470313"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)34749-5"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-15-1-154"},{"key":"e_1_2_1_7_1","author":"Nielsen J.B.K.","year":"1983","journal-title":"Biochemistry"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(71)90460-8"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(69)90288-5"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(67)90358-0"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(70)90051-3"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj0960671"},{"key":"e_1_2_1_13_1","volume-title":"Experiments in Molecular Genetics","author":"Miller J.H.","year":"1972"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.110.3.1135-1146.1972"},{"key":"e_1_2_1_15_1","author":"M&#x00E9;zes P.S.F.","year":"1983","journal-title":"J. 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