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One\u2010dimensional peptide maps and two\u2010dimensional tryptic fingerprints of the phosphoprotein from these sources were identical. A single major phosphopeptide was generated by trypsin and was determined to contain exclusively phosphothreonine. The 100 kDa substrate could be distinguished from glycogen phosphorylase (<jats:italic>M<\/jats:italic>\n<jats:sub>r<\/jats:sub> \u223c 97000) by a number of criteria including phosphopeptide mapping and by its failure to bind either to glycogen or to a specific antiphosphorylase antibody. The Ca<jats:sup>2+<\/jats:sup>\u2010dependent protein kinase responsible for phosphorylation of the 100 kDa protein appeared to be a calmodulin (CaM)\u2010requiring enzyme in that it could be inhibited in cytosol extracts by trifluoperazine (<jats:italic>IC<\/jats:italic>\n<jats:sub>50<\/jats:sub> 6\u201316 \u03bcM) and that exogenous CaM was necessary for 100 kDa phosphorylation in CaM\u2010depleted cytosol. These results suggest that a rise in intracellular Ca<jats:sup>2+<\/jats:sup> resulting in an activation of CaM\u2010dependent protein kinase leads to the phosphorylation of a common 100 kDa substrate in many tissues.<\/jats:p>","DOI":"10.1016\/0014-5793(83)81142-9","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T07:59:44Z","timestamp":1027583984000},"page":"183-190","source":"Crossref","is-referenced-by-count":46,"title":["Presence in many mammalian tissues of an identical major cytosolic substrate (<i>M<\/i><sub>r<\/sub> 100000) for calmodulin\u2010dependent protein kinase"],"prefix":"10.1002","volume":"157","author":[{"given":"H.Clive","family":"Palfrey","sequence":"first","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2001,10,29]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"220","volume-title":"Calcium and Cell Function","author":"Schulman H.","year":"1980"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)68990-8"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1979.tb04175.x"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)32934-X"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)34337-0"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(82)81231-3"},{"key":"e_1_2_1_8_1","author":"Palfrey H.C.","year":"1983","journal-title":"J. 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