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This is deduced from the observation that the drug decreases the incorporation of histidine relative to alanine into the coat protein of phage MS2, the gene of which is devoid of histidine codons. The read\u2010through of the MS2 coat cistron, due to frameshifts in vitro, is also suppressed by the antibiotic. In contrast, streptomycin enhances histidine incorporation and read\u2010through in this system. The effects of kasugamycin take place at concentrations that do not inhibit coat protein biosynthesis. Kasugamycin\u2010resistant mutants (<jats:italic>ksgA<\/jats:italic>) lacking dimethylation of two adjacent adenosines in 16 S ribosomal RNA, show an increased leakiness of nonsense and frameshift mutants (in the absence of antibiotic). They are therefore phenotypically similar to previously described ribosomal ambiguity mutants (<jats:italic>ram<\/jats:italic>).<\/jats:p>","DOI":"10.1016\/0014-5793(84)80994-1","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T09:35:43Z","timestamp":1027589743000},"page":"119-124","source":"Crossref","is-referenced-by-count":81,"title":["Increased translational fidelity caused by the antibiotic kasugamycin and ribosomal ambiguity in mutants harbouring the <i>ksgA<\/i> gene"],"prefix":"10.1002","volume":"177","author":[{"given":"C.P.J.J.","family":"van Buul","sequence":"first","affiliation":[]},{"given":"W.","family":"Visser","sequence":"additional","affiliation":[]},{"given":"P.H.","family":"van Knippenberg","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2001,11,12]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"65","volume":"19","author":"Tanaka N.","year":"1966","journal-title":"J. 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