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The solution structures of such oligonucleotides can potentially be solved using proton\u2010proton nuclear Overhauser enhancement measurements to demonstrate the proximity of protons in space and to determine their separation, thereby enabling a comparison of the structure in the crystalline and solution states to be made. In this review we describe (i) the general strategy for the sequential resonance assignments of oligonucleotide <jats:sup>1<\/jats:sup>H\u2010NMR spectra, the essential prerequisite for further structural work, (ii) the approach to obtaining interproton distances from pre\u2010steady state nuclear Overhauser enhancement measurements, and (iii) the use of interproton distances in structure determination. This is illustrated by several examples including double\u2010 and single\u2010stranded DNA oligonucleotides as well as RNA stem and loop structures.<\/jats:p>","DOI":"10.1016\/0014-5793(85)80516-0","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T07:58:50Z","timestamp":1027583930000},"page":"187-198","source":"Crossref","is-referenced-by-count":49,"title":["Probing the three\u2010dimensional structures of DNA and RNA oligonucleotides in solution by nuclear Overhauser enhancement measurements"],"prefix":"10.1002","volume":"179","author":[{"given":"G.Marius","family":"Clore","sequence":"first","affiliation":[]},{"given":"Angela M.","family":"Gronenborn","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2001,11,19]]},"reference":[{"key":"e_1_2_1_2_1","volume-title":"Chemical and Enzymatic Synthesis of Gene Fragments, A Laboratory Manual","author":"Gassen H.G.","year":"1982"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","unstructured":"Dickerson R.E. 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