{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T07:03:47Z","timestamp":1762067027628},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2001,11,2]],"date-time":"2001-11-02T00:00:00Z","timestamp":1004659200000},"content-version":"vor","delay-in-days":5996,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[1985,6,3]]},"abstract":"<jats:p>Fibroblast growth factor (FGF) has been purified to homogeneity from human brain by a procedure involving salt precipitation, cation\u2010exchange chromatography, Heparin\u2010Sepharose affinity chromatography and reverse\u2010phase HPLC. Isolation was monitored by radioimmunoassay and\/or by testing column fractions for their capacity to stimulate the proliferation of vascular endothelial cells in vitro. The amino\u2010terminal sequence of human brain FGF was, determined as Pro\u2010Ala\u2010Leu\u2010Pro\u2010Glu\u2010Asp\u2010Gly\u2010Gly\u2010Ser\u2010Gly\u2010Ala\u2010PhePro\u2010. This sequence is identical to that of the amino\u2010terminal region of bovine FGF. Additional evidence, including amino acid composition, Chromatographie retention behavior, and immunoreactivity suggest that the human and bovine mitogens are very similarin structure.<\/jats:p>","DOI":"10.1016\/0014-5793(85)80765-1","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T07:58:50Z","timestamp":1027583930000},"page":"177-181","source":"Crossref","is-referenced-by-count":51,"title":["Human brain fibroblast growth factor"],"prefix":"10.1002","volume":"185","author":[{"given":"Peter","family":"B\u00f6hlen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frederick","family":"Esch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Baird","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenneth L.","family":"Jones","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denis","family":"Gospodarowicz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,11,2]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.17.5364"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041220223"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.22.6963"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-483180-3.50008-3"},{"key":"e_1_2_1_6_1","first-page":"109","article-title":"Gene Expression and Regulation in Cultured Cells","author":"Gospodarowicz D.","year":"1978","journal-title":"National Cancer Institute Monographs"},{"key":"e_1_2_1_7_1","first-page":"33","volume-title":"Molecular Control of Proliferation and Cytodifferentiation","author":"Gospodarowicz D.","year":"1978"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4835(79)90038-1"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6199844"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)34863-9"},{"key":"e_1_2_1_11_1","author":"Baird A.","year":"1985","journal-title":"Reg. Pept."},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(82)90120-8"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(84)90305-1"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(83)91045-5"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(84)90946-X"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6382607"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.3.805"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.2.357"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.240200304"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.5.3068"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490080235"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(85)90614-X"},{"key":"e_1_2_1_23_1","author":"Gospodarowicz D.","year":"1985","journal-title":"Biochem. Biophys. Res. Commun."}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2F0014-5793%2885%2980765-1","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2F0014-5793(85)80765-1","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/0014-5793%2885%2980765-1","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T22:22:23Z","timestamp":1694902943000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1016\/0014-5793%2885%2980765-1"}},"subtitle":["Isolation and partial chemical characterization"],"short-title":[],"issued":{"date-parts":[[1985,6,3]]},"references-count":22,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1985,6,3]]}},"alternative-id":["10.1016\/0014-5793(85)80765-1"],"URL":"https:\/\/doi.org\/10.1016\/0014-5793(85)80765-1","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[1985,6,3]]}}}