{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T21:29:53Z","timestamp":1767648593529},"reference-count":18,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2001,11,2]],"date-time":"2001-11-02T00:00:00Z","timestamp":1004659200000},"content-version":"vor","delay-in-days":5555,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[1986,8,18]]},"abstract":"<jats:p>The primary structures of six phosphoglycerate kinases (PGKs) are known: three from mammals, one from yeast, and two from trypanosomes. Comparison of the amino acid sequence of these enzymes reveals 154 invariant positions out of 392 positions in the aligned sequences. Most of the conserved positions fall into the twelve \u03b2\u2010sheets and adjacent peptide regions that form the inner loops surrounding the ATP and 3\u2010phosphoglycerate\u2010binding cleft. The homology between mammalian and yeast PGKs is greater than 94% for the inner\u2010loop region, even though the overall homology is less than 65%. Trypanosome PGK has only 44% overall homology with the mammalian enzyme, but shows 74% homology in the inner\u2010loop region. Trypanosome PGK contains a polypeptide segment in its N\u2010terminal domain that is transposed in comparison with the other species.<\/jats:p>","DOI":"10.1016\/0014-5793(86)80835-3","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T07:57:40Z","timestamp":1027583860000},"page":"313-317","source":"Crossref","is-referenced-by-count":49,"title":["Evolutionary conservation of the substrate\u2010binding cleft of phosphoglycerate kinases"],"prefix":"10.1002","volume":"204","author":[{"given":"Nozomu","family":"Mori","sequence":"first","affiliation":[]},{"given":"Judith","family":"Singer-Sam","sequence":"additional","affiliation":[]},{"given":"Arthur D.","family":"Riggs","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2001,11,2]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"335","volume-title":"The Enzymes","author":"Scopes R.K.","year":"1973"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/247014a0"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1982.tb01366.x"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/279773a0"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(84)90476-5"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.2.472"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/10.23.7791"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1985.tb04152.x"},{"key":"e_1_2_1_10_1","volume-title":"Molecular Cloning: a Laboratory Manual","author":"Maniatis T.","year":"1982"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.3.802"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/250194a0"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1874-6047(08)60210-3"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(77)80048-X"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.220706"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(79)90308-5"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/261552a0"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/268495a0"},{"key":"e_1_2_1_19_1","author":"Mori N.","year":"1986","journal-title":"Gene"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2F0014-5793%2886%2980835-3","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2F0014-5793(86)80835-3","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/0014-5793%2886%2980835-3","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T14:34:51Z","timestamp":1694874891000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1016\/0014-5793%2886%2980835-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1986,8,18]]},"references-count":18,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1986,8,18]]}},"alternative-id":["10.1016\/0014-5793(86)80835-3"],"URL":"https:\/\/doi.org\/10.1016\/0014-5793(86)80835-3","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[1986,8,18]]}}}