{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,17]],"date-time":"2023-09-17T09:43:40Z","timestamp":1694943820752},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2001,10,19]],"date-time":"2001-10-19T00:00:00Z","timestamp":1003449600000},"content-version":"vor","delay-in-days":5198,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[1987,7,27]]},"abstract":"<jats:p>The non\u2010hydrolyzable GTP analogue, guanosine 5\u2032\u2010<jats:italic>O<\/jats:italic>\u2010(3\u2010thiotriphosphate) (GTP\u03b3S) and cyclic AMP potentiated the Ca<jats:sup>2+<\/jats:sup>\u2010evoked secretion of \u03b1\u2010melanocyte\u2010stimulating hormone (\u03b1\u2010MSH) from permeabilized neurointermediate lobe (IL) cells of rat pituitary gland. The enhancement by Mg\u2010GTP\u03b3S (100 \u03bcM) and cyclic AMP (1 \u03bcM) depended on the intracellular CA<jats:sup>2+<\/jats:sup> concentration (EC<jats:sub>50<\/jats:sub> = 4.8 \u00b1 1.8 and 4.6 \u00b1 1.7 \u03bcM; mean \u00b1 SE, with and without Mg\u2010GTP\u03b3S and cyclic AMP, respectively). A similar effect was observed with guanine nucleotide triphosphate (GTP and GppNHp). Mg was absolutely required for this event. Neither Mg\u2010GTP\u03b3S nor cyclic AMP alone was effective in potentiating \u03b1\u2010MSH secretion. GDP\u03b2S blocked the Mg\u2010GTP\u03b3S (100 \u03bcM) and cyclic AMP augmented secretion of \u03b1\u2010MSH. Neither neomycin (which affects the process of inositol 1,4,5\u2010triphosphate\u2010mediated Ca<jats:sup>2+<\/jats:sup> mobilization) or colchicine (which influences microtubule assembly) had an effect on the cyclic AMP and Mg\u2010GTP\u03b3S potentiation of \u03b1\u2010MSH secretion. These data suggest that the GTP\u2010binding protein may be involved in the regulation of \u03b1\u2010MSH secretion after Ca<jats:sup>2+<\/jats:sup> entry into the cells, since the intracellular environment is controlled in the permeabilized cells.<\/jats:p>","DOI":"10.1016\/0014-5793(87)80245-4","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T07:57:40Z","timestamp":1027583860000},"page":"326-330","source":"Crossref","is-referenced-by-count":6,"title":["\u03b1\u2010Melanocyte\u2010stimulating hormone secretion from permeabilized intermediate lobe cells of rat pituitary gland The role of guanine nucleotides"],"prefix":"10.1002","volume":"219","author":[{"given":"Toshifumi","family":"Yamamoto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihiro","family":"Furuki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John W.","family":"Kebabian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Spatz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,10,19]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.1.152"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(20)82047-X"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/284017a0"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)44427-9"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(84)90336-2"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)32507-9"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)32881-3"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.2.462"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.22.7500"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00538a029"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/310644a0"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/314534a0"},{"key":"e_1_2_1_14_1","author":"Yamamoto T.","year":"1987","journal-title":"Biochem. 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