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The antibody preparation reacted with another membrane protein from methicillin\u2010sensitive <jats:italic>S. aureus<\/jats:italic> (MSSA) which migrated to the same position as PBP 2' on SDS gel electrophoresis. Pretreatment of the antisera with membranes of sensitive strains removed antibodies recognising high\u2010<jats:italic>M<\/jats:italic>\n<jats:sub>r<\/jats:sub>, proteins common to both MRSA and MSSA: the residual antibody then reacted specifically with PBP 2'. 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