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The \u03b1\u2010subunit of the purified protein (\u03b1<jats:sub>40<\/jats:sub>\u03b2\u03b3) had a molecular mass of 40 kDa and differed from that of G<jats:sub>i<\/jats:sub>(\u03b1<jats:sub>41<\/jats:sub>\u03b2\u03b3) or G<jats:sub>o<\/jats:sub>(\u03b1<jats:sub>39<\/jats:sub>\u03b2\u03b3) previously purified from brain tissues. The fragmentation patterns of limited tryptic digestion and immunological cross\u2010reactivities among the three \u03b1 were different from one another. However, the \u03b2\u03b3\u2010subunit resolved from the three IAP substrates similarly inhibited a membrane\u2010bound adenylate cyclase and their \u03b2\u2010subunits were immunologically indistinguishable from one another. Thus, the \u03b1<jats:sub>40<\/jats:sub>\u03b2\u03b3 is a new IAP substrate protein different from G<jats:sub>i<\/jats:sub> or G<jats:sub>o<\/jats:sub>, in the \u03b1\u2010subunit only.<\/jats:p>","DOI":"10.1016\/0014-5793(87)81521-1","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T07:57:40Z","timestamp":1027583860000},"page":"353-358","source":"Crossref","is-referenced-by-count":149,"title":["A new GTP\u2010binding protein in brain tissues serving as the specific substrate of islet\u2010activating protein, pertussis toxin"],"prefix":"10.1002","volume":"213","author":[{"given":"Toshiaki","family":"Katada","sequence":"first","affiliation":[]},{"given":"Masayuki","family":"Oinuma","sequence":"additional","affiliation":[]},{"given":"Kayoko","family":"Kusakabe","sequence":"additional","affiliation":[]},{"given":"Michio","family":"Ui","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2001,11,2]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-2236(86)90148-7"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.1.152"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)89817-9"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)89881-7"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)89515-1"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)83894-2"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-6147(84)90444-9"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)83662-1"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)89826-X"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)88845-7"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)67279-5"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/317536a0"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00347a003"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)89236-0"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)43132-2"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(86)80007-2"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2F0014-5793%2887%2981521-1","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2F0014-5793(87)81521-1","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/0014-5793%2887%2981521-1","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T20:11:54Z","timestamp":1694895114000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1016\/0014-5793%2887%2981521-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,3,23]]},"references-count":16,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1987,3,23]]}},"alternative-id":["10.1016\/0014-5793(87)81521-1"],"URL":"https:\/\/doi.org\/10.1016\/0014-5793(87)81521-1","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,3,23]]}}}