{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T05:40:51Z","timestamp":1774676451357,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2001,10,19]],"date-time":"2001-10-19T00:00:00Z","timestamp":1003449600000},"content-version":"vor","delay-in-days":4743,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[1988,10,24]]},"abstract":"<jats:p>Human immunodeficiency virus (HIV) was purified by sucrose gradient centrifugation and labeled with octadecylrhodamine B\u2010chloride (R\u201018) under conditions resulting in 90% quenching of the fluorescence label. Incubation of R\u201018\u2010labeled HIV (R\u201018\/HIV) with CD4\u2010positive CEM and HUT\u2010102 cells, but not with CD4\u2010negative MLA\u2010144 cells, resulted in fluorescence dequenching (DQ, increase in fluorescence) of 20\u201325%. Similar level of DQ was observed upon incubation of CEM cells with R\u201018\u2010labeled Sendai virus. DQ was observed when R\u201018\/HIV was incubated with CD4<jats:sup>+<\/jats:sup> cells at 37\u00b0C, but not at 4\u00b0C. Most of the increase in fluorescence occurred within 5 min of incubation at 37\u00b0C and was independent of medium pH over the range of pH 5\u20138. Preincubation of cells with the lysosomotropic agent NH<jats:sub>4<\/jats:sub>Cl had no inhibitory effect on DQ. Complete inhibition was observed when target cells were fixed with glutaraldehyde prior to R\u201018\/HIV addition. Our results demonstrate application of membrane fluorescence dequenching method to a quantitative measurement of fusion between HIV and target cell membranes. As determined by DQ, HIV penetrates into target cells by a rapid, pH\u2010independent, receptor\u2010mediated and specific process of fusion between viral envelope and cell plasma membrane, quite similar to that observed with Sendai virus.<\/jats:p>","DOI":"10.1016\/0014-5793(88)80551-9","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T07:45:33Z","timestamp":1027583133000},"page":"88-92","source":"Crossref","is-referenced-by-count":59,"title":["Quantitative measurement of fusion between human immunodeficiency virus and cultured cells using membrane fluorescence dequenching"],"prefix":"10.1002","volume":"239","author":[{"given":"Faruk","family":"Sinangil","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abraham","family":"Loyter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.J.","family":"Volsky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,10,19]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6189183"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6200936"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6206563"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6328660"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3277274"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(86)90590-8"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(87)90542-3"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1988.tb02839.x"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.62.1.139-147.1988"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2830667"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3014663"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.18.7023"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3010463"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)38987-1"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)67334-X"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)68847-7"},{"key":"e_1_2_1_18_1","first-page":"33","author":"Loyter A.","year":"1988","journal-title":"Biochem. 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