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Exons 2, 6 and 7 encode putative guanine nucleotide\u2010binding domains that are highly conserved among GTP\u2010binding proteins. A polyadenylation signal located within exon 9 predicts an MRNA size (\u223c 2.3 kb) several hundred bases longer than that of published cDNAs, and consistent with the size seen on RNA blot hybridization. Primer extension and S<jats:sub>1<\/jats:sub> nuclease analysis determined a major and several minor transcriptional start sites. The first exon and 5\u2032 flanking region are highly G+ C rich, contain several GC boxes (SP1 transcription factor binding sites), a CAAT box, and lack a TATA box. The presumptive promoter region is thus similar to that of <jats:italic>ras<\/jats:italic> and other widely expressed genes.<\/jats:p>","DOI":"10.1016\/0014-5793(88)80764-6","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T07:45:58Z","timestamp":1027583158000},"page":"333-340","source":"Crossref","is-referenced-by-count":38,"title":["Cloning and characterization of the human gene for the \u03b1\u2010subunit of G<sub>i2<\/sub>, a GTP\u2010binding signal transduction protein"],"prefix":"10.1002","volume":"232","author":[{"given":"L.S.","family":"Weinstein","sequence":"first","affiliation":[]},{"given":"A.M.","family":"Spiegel","sequence":"additional","affiliation":[]},{"given":"A.D.","family":"Carter","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2001,11,2]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0303-7207(87)90058-X"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.56.070187.003151"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(87)80218-1"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)47929-X"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)47849-0"},{"key":"e_1_2_1_7_1","author":"Polakis P.G.","year":"1988","journal-title":"J. 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